The Guardians of the TWiV-o-verse discuss the process of codon deoptimization for the production of experimental infectious attenuated viral vaccines, and how a respiratory syncytial virus with 619 base changes was rescued by genomes with very large internal deletions. Hosts: Vincent Racaniello, Dickson Despommier , Rich Condit , Kathy Spindler , and Brianne Barker Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode NIDO 2021 Symposium ASV hos...
Apr 08, 2021•1 hr 37 min•Ep. 740
Arturo Casadevall joins TWiV to discuss the use of convalescent plasma to treat COVID-19, including a history of the process, how plasma is prepared and tested, why it is difficult to conduct randomized clinical trials, how this treatment has fared and his hopes for the future. Hosts: Vincent Racaniello, Alan Dove , Rich Condit , and Brianne Barker Guest: Arturo Casadevall Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode Victory for Scienti...
Apr 04, 2021•1 hr 55 min•Ep. 739
In COVID-19 clinical update #56, Daniel Griffin note increasing hospitalization in children and young adults, Pfizer vaccine efficacy results in 12-15 year olds, rapid self administered nucleic acid and antigen tests, mRNA vaccine efficacy in real world conditions, and improvement of long COVID after vaccination. Hosts: Daniel Griffin and Vincent Racaniello Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode Pfizer mRNA vaccine is 100% effecti...
Apr 03, 2021•35 min•Ep. 738
John, Monica, and Davida join TWiV to discuss development of a method to detect SARS-CoV-2 in raw sewage from all 14 of New York City's treatment plants, and its use to determine the presence of the genome throughout the pandemic and detection of genome mutations from variants of concern. Hosts: Vincent Racaniello, Dickson Despommier , Rich Condit , and Kathy Spindler Guest: John Dennehy , Davida Smyth , and Monica Trujillo Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron...
Apr 01, 2021•1 hr 55 min•Ep. 737
Alessandro Sette joins TWiV to discuss the role of T cells in COVID-19, the finding that amino acid changes in SARS-CoV-2 variants of concern have a negligible impact on T cell reactivity in convalescent patients and vaccinees, and whether a next generation vaccine should include more viral proteins than spike. Hosts: Vincent Racaniello, Alan Dove , Rich Condit , and Kathy Spindler Guest: Alessandro Sette Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for...
Mar 28, 2021•2 hr 2 min•Ep. 736
In COVID-19 clinical update #55, Daniel Griffin reviews recommendations for keeping transmission low in school settings, optimal testing strategies for schools and businesses, impact of vaccines on asymptomatic infections, vaccine safety in PASC patients, and early use of aspirin associated with decreased mortality. Hosts: Daniel Griffin and Vincent Racaniello Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode COVID-19 in primary and secondar...
Mar 26, 2021•40 min•Ep. 735
Susan Weiss returns to TWiV to discuss coronavirus entry into cells and the role of spike protein cleavage by proteases, why lab escaped hypotheses for SARS-CoV-2 are unlikely, and modulation of innate immune responses during infection. Hosts: Vincent Racaniello, Rich Condit , Alan Dove , Brianne Barker , and Amy Rosenfeld Guest: Susan Weiss Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode CoV pathogenesis in CNS (J Neuro Pharm) SARS-CoV-2 ...
Mar 21, 2021•2 hr 11 min•Ep. 734
In COVID-19 clinical update #54, Daniel Griffin covers effectiveness of 3 vs 6 ft physical distancing, effectiveness of masks, physical distancing, and eye shields, second vaccine dose completion, alleviation of COVID-19 symptoms after vaccination, vaccine trials in children, and IDSA approval of a mAb cocktail. Hosts: Daniel Griffin and Vincent Racaniello Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode Effectiveness of 3 ft vs 6 ft (CID) ...
Mar 20, 2021•34 min•Ep. 733
Forest and Maria-Isabel join TWiV to discuss their their project to engage thousands of citizens to swab inanimate surfaces in the San Diego area, then send the samples to the Rohwer Lab for nucleic acid analysis. Hosts: Vincent Racaniello, Dickson Despommier , Rich Condit , Kathy Spindler , and Brianne Barker Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode Forest on TWiV 391 Swabbing surfaces in San Diego (10News) Nido21 meeting Molnupira...
Mar 18, 2021•1 hr 52 min•Ep. 732
On a TWiV tetramer, we review the ongoing outbreaks of Ebolavirus disease in DRC and Guinea, evidence that SARS-CoV-2 infections in children are far more prevalent than are being detected, and recent long COVID estimates from the UK. Hosts: Vincent Racaniello, Rich Condit , Alan Dove , and Brianne Barker Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode Ebolavirus disease outbreak continues in Africa (ProMedMail) List of Ebolavirus outbreaks...
Mar 14, 2021•1 hr 55 min•Ep. 731
In COVID-19 clinical update #53, Daniel Griffin reviews underreporting of infections and long term disease in children, a blood test for T cells, results of phase 3 trials for monoclonal antibodies, preliminary findings on an oral antiviral, clinical trial of ivermectin, and recommendations for upcoming gatherings. Hosts: Daniel Griffin and Vincent Racaniello Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode SARS-CoV-2 seroprevalence in EUA ...
Mar 13, 2021•45 min•Ep. 730
Matt Frieman returns to TWiV to discuss the COVID-19 pandemic from the point of view of a coronavirologist, including his work with Novavax on their spike protein-based vaccine. Hosts: Vincent Racaniello, Dickson Despommier , Rich Condit , Kathy Spindler , and Brianne Barker Guest: Matt Frieman Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode Phase 1-2 trial of Novavax protein vaccine (NEJM) MERS-CoV spike nanoparticle vaccine in mice (Vacc...
Mar 11, 2021•1 hr 57 min•Ep. 729
TWiV notes the passing of Mavis Agbandje-McKenna, and reviews data on maximizing fit for cloth and medical procedure masks, SARS-CoV-2 total and subgenomic viral load in hospitalized patients, and effect of changes in spike protein on efficacy in a mouse model of infection. Hosts: Vincent Racaniello, Dickson Despommier , Alan Dove , Rich Condit , and Brianne Barker Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode Mavis Agbandje-McKenna dies...
Mar 07, 2021•2 hr 12 min•Ep. 728
In COVID-19 clinical update #52, Daniel Griffin reviews differences in attack rates between children and adults, J&J vaccine phase III data, G6PD deficiency and vaccines, NIH halts convalescent plasma trial, dexamethasone in hospitalized patients, data on tocilizumab, and long COVID in children. Hosts: Daniel Griffin and Vincent Racaniello Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode SARS-CoV-2 attack rates in children and adults (C...
Mar 06, 2021•35 min•Ep. 727
Janet Iwasa joins TWiV to recount how she became a molecular animator, including a pivot during her Ph.D. thesis research, the value of animations in science, and their latest animation of SARS-CoV-2 entry into cells. Hosts: Vincent Racaniello, Rich Condit , Brianne Barker and Kathy Spindler Guest: Janet Iwasa Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode The Animation Lab SARS-CoV-2 animation Documents for J&J COVID-19 vaccine revie...
Mar 04, 2021•1 hr 53 min•Ep. 726
Eva Harris and Janet Smith join TWiV to discuss how an antibody against dengue virus NS1 protein blocks endothelial dysfunction and the potential of treating infections by multiple flaviviruses. Hosts: Vincent Racaniello, Alan Dove , Rich Condit , and Kathy Spindler Guest: Eva Harris and Janet Smith Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode Eva Harris on TWiV 228 Zika virus in Nicaragua (virology blog) How an antibody block NS1-media...
Feb 28, 2021•1 hr 59 min•Ep. 725
In COVID-19 clinical update #51, Daniel Griffin reviews use of two masks, effectiveness of testing in nursing homes, updates on vaccines, use of nonsteroidal anti-inflammatory drugs, little effect of vitamin D and remdesivir in clinical studies, and a long COVID research initiative from NIH. Hosts: Daniel Griffin and Vincent Racaniello Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode Updated NYC mask guidance (NYC Health) Two masks recommen...
Feb 27, 2021•37 min•Ep. 724
Ginger Campbell joins TWiV to discuss palliative care for COVID-19 patients, and how to approach the disease from a goals of care perspective. Hosts: Vincent Racaniello, Dickson Despommier , Rich Condit , and Brianne Barker Guest: Ginger Campbell, MD Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode Brain Science Podcast Dr. Campbell's video on palliative care (YouTube) Resources on palliative care (WHO) Interviews with Paul Offit ( one , tw...
Feb 25, 2021•1 hr 48 min•Ep. 723
The entire TWiV team assembles to discuss resurgence of COVID-19 in Manaus, Brazil, discovery of SARS-CoV-2 related viruses in bats in Southeast Asia, and increased Ct values after administration of mRNA vaccine. Hosts: Vincent Racaniello, Dickson Despommier , Alan Dove , Rich Condit , Kathy Spindler , and Brianne Barker Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode Resurgence of COVID-19 in Manaus (Lancet) SARS-CoV-2 related viruses in ...
Feb 21, 2021•2 hr 2 min•Ep. 722
In COVID-19 clinical update #50, Daniel Griffin discusses CDC guidance on school opening, decreased viral load after vaccination, randomized clinical trial of vitamin D treatment, a study of zinc plus vitamin C, anticoagulation therapy effect on mortality, and multi system inflammatory phase in children. Hosts: Daniel Griffin and Vincent Racaniello Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode Guidance on school openings (CDC) Calcifedio...
Feb 20, 2021•30 min•Ep. 721
Paul Offit joins TWiV to discuss all things vaccine, including how COVID-19 vaccine development was accelerated, whether the vaccines are safe and efficacious, the difference between an EUA and a licensed vaccine, sterilizing immunity, herd immunity, and much more. Hosts: Vincent Racaniello, Dickson Despommier , Alan Dove , Rich Condit , and Brianne Barker Guest: Paul Offit Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode Vaccine education ...
Feb 14, 2021•2 hr 1 min•Ep. 720
In COVID-19 clinical update #49, Daniel Griffin discusses CDC guidelines on mask use, drop in test frequency in the US, resurgence of COVID-19 in Brazil, possible impact of Pfizer vaccine on viral load, EUA for bamlanivimab and etesevimab administered together, and preliminary results of Tocilizumab in hospitalized patients. Hosts: Daniel Griffin and Vincent Racaniello Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode Resurgence of COVID-19 ...
Feb 13, 2021•28 min•Ep. 719
TWiV explores single dose administration and timing of second dose of AZD1222 COVID-19 vaccine, safety and efficacy of Sputnik V, and control of coronavirus recombination by a viral proofreading exonuclease. Hosts: Vincent Racaniello, Rich Condit , Kathy Spindler , and Brianne Barker Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode AZD1222 single dose efficacy and booster timing (Lancet preprint) Safety and efficacy of Sputnik V vaccine (La...
Feb 11, 2021•1 hr 52 min•Ep. 718
Theodora and Paul return to TWiV to explain how they quickly pivoted their retrovirus laboratories towards SARS-CoV-2, their work on the antibody response to infection and its durability, and the emergence of variants with increased resistance to neutralization. Hosts: Vincent Racaniello, Dickson Despommier , Alan Dove , and Rich Condit Guests: Paul Bieniasz and Theodora Hatziioannou Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode Evolutio...
Feb 07, 2021•1 hr 57 min•Ep. 717
In COVID-19 clinical update #48, Daniel Griffin reviews effect of spike amino acid changes on neutralization with monoclonal antibodies, Sputnik V safety and efficacy results, increased reactogenicity after a single vaccine dose in seropositive individuals, single dose vaccination in seropositive health care workers, and results of three clinical trials on the use of anticoagulants. Hosts: Daniel Griffin and Vincent Racaniello Subscribe (free): iTunes , Google Podcasts , RSS , email Become a pat...
Feb 06, 2021•52 min•Ep. 716
TWiV explains why hydroxychloroquine failed in humans despite showing antiviral effects in cells, and reviews the published data on the Pfizer/BioNTech mRNA vaccine. Hosts: Vincent Racaniello, Rich Condit , Kathy Spindler , and Brianne Barker Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode TMPRSS2 is why hydroxychloroquine failed (PLoS Path) Safety and efficacy of BNT162b2 mRNA vaccine (NEJM) Letters read on TWiV 715 Timestamps by Jolene ....
Feb 04, 2021•1 hr 50 min•Ep. 715
Jason McLellan joins TWiV to reveal all we know about the SARS-CoV-2 spike protein, followed by Novavax and J&S phase 3 results and a discussion of variants of concern: neutralization by vaccine-induced antibodies, transmission, and virulence. Hosts: Vincent Racaniello, Dickson Despommier , Alan Dove , Rich Condit , and Amy Rosenfeld Guest: Jason McLellan Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode Prefusion spike structure (Scienc...
Jan 31, 2021•2 hr 7 min•Ep. 714
In COVID-19 clinical update #47, Daniel Griffin reviews phase 3 results for Novavax and J&J vaccines, transmission in K-12 settings in Wisconsin, data on Lilly and Regeneron monoclonal antibody therapy, and a study of colchicine for treatment of disease. Hosts: Daniel Griffin and Vincent Racaniello Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode Novavax vaccine phase 3 results (Novavax) J&J vaccine phase 3 results (J&J) COVID-1...
Jan 30, 2021•35 min•Ep. 713
Gisa, Steffi, and César join TWiV to discuss their careers, their research, and the COVID-19 pandemic in Germany. Hosts: Vincent Racaniello, Rich Condit , and Kathy Spindler Guests: Gisa Gerold , Stefanie Becker , and César Muñoz-Fontela Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode German Society for Virology Ticks in Germany and TBEV (Sci Rep) Timestamps by Jolene . Thanks! Intro music is by Ronald Jenkees Send your virology questions ...
Jan 28, 2021•1 hr 38 min•Ep. 712
The TWiVers review Biden's national strategy for the COVID-19 responses and pandemic preparedness, and an evaluation of the BinaxNOW rapid antigen test for SARS-CoV-2 infection. Hosts: Vincent Racaniello, Dickson Despommier , Alan Dove , Rich Condit , and Brianne Barker Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode Biden's COVID-19 strategy ( pdf ) CDC evaluation of BinaxNOW antigen test (MMWR) FDA instructions for BinaxNOW test ( pdf ) ...
Jan 24, 2021•2 hr•Ep. 711
