TWiV explains that a recent report suggesting that the SARS-CoV-2 spike protein inhibits V(D)J recombination in vitro would not impact immunity after infection or vaccination, and describes the isolation of remdesivir resistant mutants in cells in culture, and the emergence of amino acid changes in the spike protein identical to those in variants of concern, in the absence of immune selection. Hosts: Vincent Racaniello , Alan Dove , Rich Condi t , and Kathy Spindler Click arrow to play Download ...
Nov 18, 2021•2 hr 8 min•Ep. 830
A TWiV duo reviews how SARS-CoV-2 attacks the olfactory mucosa but spares the olfactory bulb, vaccination with BNT162b2 induces virus-specific stem cell memory T cells, and development of an oral protease inhibitor for the treatment of COVID-19. Hosts: Vincent Racaniello and Brianne Barker Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode How SARS-CoV-2 attacks olfactory mucosa (Cell) SARS-CoV-2-specific stem cell memory T cells after mRNA v...
Nov 14, 2021•1 hr 44 min•Ep. 829
In COVID-19 clinical update #88, Dr. Griffin covers vaccine protection among US veterans, absence of long COVID in vaccinated who are infected, single dose of REGEN-COV monoclonal antibody cocktail provides long term protection, and Pfizer protease inhibitor Paxlovid is 89% effective in preventing hospitalization or death. Hosts: Daniel Griffin and Vincent Racaniello Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode Vaccine protection of US ...
Nov 13, 2021•33 min•Ep. 828
Greg Zuckerman joins TWiV to discuss his book that explores the race to produce COVID-19 vaccines, with insights into the companies, scientists, and executives involved and the rivalries, ambitions, ego, and a desire to save the world. Hosts: Vincent Racaniello , Dickson Despommier , Rich Condi t , and Kathy Spindler Guest: Greg Zuckerman Click arrow to play Download TWiV 824 (76 MB .mp3, 127 min) Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this ep...
Nov 07, 2021•1 hr 52 min•Ep. 827
In COVID-19 clinical update #87, Dr. Griffin reviews PCR cycle threshold and RNA copy number, rapid antigen tests, hospitalization rate after infection or vaccination, allergies and vaccination, community transmission and viral RNA load, early treatment with sotrovimab, meta-analysis of tocilizumab treatment, antibiotic overuse, and guidelines on the use of anticoagulation for thromboprophylaxis. Hosts: Daniel Griffin and Vincent Racaniello Subscribe (free): iTunes , Google Podcasts , RSS , emai...
Nov 06, 2021•49 min•Ep. 826
In this special live-streamed episode of TWiV, Dr. Daniel Griffin fields questions on SARS-CoV-2 and COVID-19 from listeners. Hosts: Daniel Griffin and Vincent Racaniello Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Intro music is by Ronald Jenkees Send your questions for Dr. Griffin to daniel@microbe.tv...
Nov 01, 2021•58 min•Ep. 825
TWiV explores the properties of the spike glycoproteins of an influenza B virus discovered in the Wuhan spiny eel, and protection against SARS-CoV-2 infection one year after mRNA-1273 vaccination of nonhuman primates. Hosts: Vincent Racaniello , Dickson Despommier , Rich Condi t , and Brianne Barker Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode HA and NA of Wuhan spiny eel influenza virus (Nat Comm) Protection one year after mRNA-1273 va...
Oct 31, 2021•2 hr 7 min•Ep. 824
In COVID-19 clinical update #86, Dr. Griffin discusses virologic features of infection in children, antibody tests should not be used to asses level of protection, test-to-stay programs in schools, FDA authorizes Moderna boosters, results of TOGETHER trial for fluvoxamine, statins and 28 day mortality, and cognitive function in patients. Hosts: Daniel Griffin and Vincent Racaniello Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode 115,000 he...
Oct 30, 2021•47 min•Ep. 823
Rich reviews the history of smallpox and the discovery of variola viral genomes in ancient DNA from human remains throughout Northern Europe, which pushes back the earliest date of smallpox virus infection to ~600–1050 CE, overlapping the Viking Age. Hosts: Vincent Racaniello , Alan Dove , Rich Condi t , Kathy Spindler , and Brianne Barker Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode Variola virus genomes from Viking era (Science) Hidde...
Oct 28, 2021•2 hr 1 min•Ep. 822
A TWiV trio reveal the isolation of novel paramyxoviruses from rodents and bats in Arizona, and isolation of naive B cells from seronegative donors that produce germline encoded antibodies which engage the receptor binding domain of SARS-CoV-2, variants of concern, and related sarbecoviruses from bats. Hosts: Vincent Racaniello , Rich Condi t , and Brianne Barker Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode Bat and rodent paramyxoviruse...
Oct 24, 2021•2 hr 1 min•Ep. 821
In COVID-19 clinical update #85, Dr. Griffin reviews cases and deaths by vaccination status, mRNA vaccine effectiveness in 12-18 year olds, standard and extended dosing of mRNA vaccines, immune responses after vaccination, Moderna booster dose approved, giving monoclonals at home, no benefit from colchicine, and IFN does not help remdesivir. Hosts: Daniel Griffin and Vincent Racaniello Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode Guidel...
Oct 23, 2021•41 min•Ep. 820
TWiV explains how remdesivir inhibits the SARS-CoV-2 RNA polymerase: the drug is incorporated into the growing RNA chain and causes synthesis to stall when the drug clashes with an amino acid in the active site. Hosts: Vincent Racaniello , Alan Dove , Rich Condi t , and Kathy Spindler Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode How remdesivir stalls the SARS-CoV-2 polymerase (Nat Comm) Movie of polymerase stalling (Nat Comm) Second mec...
Oct 21, 2021•1 hr 41 min•Ep. 819
The TWiVers review phase 3 efficacy and safety results for the Novavax COVID-19 vaccine, effects on the neonatal immune system caused by maternal infection with SARS-CoV-2, and NHC, the metabolite of molnupiravir, causes mutations in cellular DNA. Hosts: Vincent Racaniello , Dickson Despommier , Rich Condi t , Kathy Spindler , and Brianne Barker Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode Efficacy and safety of Novavax vaccine (medRxiv...
Oct 17, 2021•1 hr 56 min•Ep. 818
In COVID-19 clinical update #84, Dr. Griffin discusses childrens' antibody response to vaccination, phase 3 results of Novavax vaccine, heterologous vaccination, antithrombotic therapy, EUA for molnupiravir, phase 3 results of AZD7442, serum ferritin levels and outcomes, heparin therapy, and WHO strategy for global vaccination. Hosts: Daniel Griffin and Vincent Racaniello Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode Antibody profiles af...
Oct 16, 2021•46 min•Ep. 817
David Tuller returns to TWiV to revisit his work to expose the methodological and ethical problems with the PACE trial, and the post-acute sequelae to SARS-CoV-2 infection, also known as long COVID. Host: Vincent Racaniello Guest: David Tuller Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode Call me David Tuller ( TWiV Special ) David writes at virology blog Timestamps by Jolene . Thanks! Intro music is by Ronald Jenkees Send your virology ...
Oct 15, 2021•1 hr 10 min•Ep. 816
Moshe and Ivet join TWiV to discuss their finding that MIS-C is a consequence of a superantigen in the spike protein of SARS-CoV-2 that causes activation of a skewed population of T cell receptor bearing lymphocytes, leading to hyperinflammation. Hosts: Vincent Racaniello , Dickson Despommier , Rich Condi t and Kathy Spindler Guests: Moshe Arditi and Ivet Behar Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode MIS-C in US children (NEJM) Sup...
Oct 10, 2021•2 hr 3 min•Ep. 815
In COVID-19 clinical update #83, Daniel Griffin discusses upcoming FDA discussions on vaccines, strategies to prevent transmission at youth camps, outbreaks at summer camps, impact of vaccination on transmission, AZD7442 request for EUA filed, EUA issued for home antigen test, phase 3 data on AZD1222 vaccine, 6 month effectiveness of Pfizer/BioNTech vaccine, estimates of durability of immunity against reinfection, Molnupiravir interim results, optimal time for monoclonal antibody infusion, IVIG ...
Oct 09, 2021•50 min•Ep. 814
From the 2021 online meeting of the European Society for Clinical Virology, Vincent speaks with meeting speakers on COVID-19 vaccines, immunity, how countries responded to the pandemic, and how to prepare for the next one. Host: Vincent Racaniello Guests: Sarah Gilbert , Florian Krammer , Fausto Baldanti , Thea Fischer , Ron Fouchier , and Catherine Moore Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode Correlate of protection needed for CO...
Oct 07, 2021•2 hr•Ep. 813
TWiV considers lessons learned from COVID-19 about emergency drug use during a pandemic, and selection of AAV capsids to produce vectors that efficiently deliver genes to muscle cells. Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Rich Condi t and Brianne Barker Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode Emergency drug use during a pandemic (Cell) Mus cle-directed AAV capsids (Cell) Designer AAV muscles up (Cell) Letter...
Oct 03, 2021•1 hr 52 min•Ep. 812
In COVID-19 clinical update #82, Daniel Griffin reviews vaccines for 5-12 years old, safety of a third vaccine dose, Oklahoma reinfection data, Regencov outcomes, Pfizer protease inhibitor, impact of Tocilizumab, and incidence of long COVID. Hosts: Daniel Griffin and Vincent Racaniello Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode Data on Pfizer-BioNTech in 5-12 year olds (Pfizer) Safety of additional vaccine dose (MMWR) Oklahoma reinfec...
Oct 02, 2021•40 min•Ep. 811
TWiV reviews how a novel technology enabled development of COVID-19 mRNA vaccines, and discovery of a proton gradient and an incomplete TCA cycle in a giant. virus. Hosts: Vincent Racaniello , Rich Condit , Kathy Spindler , and Brianne Barker Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode mRNA vaccines, a novel technology (Cell) Proton gradient in giant virus (ISME J) Tomograms of Pandoravirus massiliensis Pandoraviruses are not alive (vi...
Sep 30, 2021•2 hr•Ep. 810
TWiV reveals isolation from bats in Laos of a SARS-CoV-2 like virus with a spike protein that allows entry into human cells, and exploration of a herpesvirus vector for immunization of vampire bats against rabies virus. Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Rich Condit and Amy Rosenfeld Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode SARS-CoV-2 like viruses from Laotian bats (Res Sq) Herpesvirus in rabies endemic vam...
Sep 26, 2021•1 hr 40 min•Ep. 809
In COVID-19 clinical update #81, Daniel Griffin discusses vaccine boosters, vaccination during pregnancy, mRNA vaccine comparison, J&J vaccine booster, EUA expansion for Lilly monoclonals, remdesivir reduces risk of hospitalization, and anticoagulation regimens. Hosts: Daniel Griffin and Vincent Racaniello Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode Pfizer phase III results in 5-11 year olds (Pfizer) FDA authorizes booster (FDA) V-...
Sep 25, 2021•52 min•Ep. 808
Chadi and Caroline join TWiV to describe the potential epidemiological and evolutionary impacts of vaccine nationalism, and their modeling which emphasizes the importance of rapid equitable vaccine distribution for global control of the pandemic. Hosts: Vincent Racaniello , Dickson Despommier , Rich Condit and Brianne Barker Guests: Chadi Saad-Roy and Caroline Wagner Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode Vaccine nationalism and c...
Sep 19, 2021•1 hr 42 min•Ep. 807
In COVID-19 clinical update #80, Daniel Griffin reviews infections in children, masking, testing to limit transmission, high barrier for monoclonal antibody escape, are boosters needed, use of steroids, grants for long COVID, and how the pandemic unfolds in Africa. Hosts: Daniel Griffin and Vincent Racaniello Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode Hospitalization pre- and post-vaccines (Res Sq) Infection in children (medRxiv) Upda...
Sep 18, 2021•49 min•Ep. 806
The TWiVotopes review current evidence which does not show the need for widespread COVID-19 booster vaccination, and results of experiments to examine the replication and immune evasion of the SARS-CoV-2 delta variant. Hosts: Vincent Racaniello , Rich Condit , Kathy Spindler , and Brianne Barker Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode ASV Vaccine Town Halls Vaccine boosters not needed (Lancet) Delta variant replication and immune e...
Sep 16, 2021•1 hr 34 min•Ep. 805
Virus hunter Mya Breitbart joins TWiV to describe her laboratory's work on viruses in spiders, insects, millipedes, freshwater springs, seawater, plants, human feces and much more. Hosts: Vincent Racaniello , Alan Dove , and Dickson Despommier Guest: Mya Breitbart Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode Viruses of spinybacked orbweavers (J Gen Virol) Viruses of terrestrial arthropods (PeerJ) Pepper mild mottle virus in sewage (PLoS...
Sep 12, 2021•1 hr 51 min•Ep. 804
In COVID-19 clinical update #79, Daniel Griffin discusses clinical management guidelines, ER visits and hospitalization among children, infection and vaccine induced seroprevalence in the US, antibodies in Kenyan blood donors, infections after vaccination, anticoagulation for thromboprophylaxis, baracitinib efficacy, long-term symptoms in adults, and sustained symptoms after severe disease. Hosts: Daniel Griffin and Vincent Racaniello Subscribe (free): iTunes , Google Podcasts , RSS , email Beco...
Sep 11, 2021•45 min•Ep. 803
Shane Crotty returns to TWiV to review the immunology of COVID-19, including differences between infection and vaccination, increased breadth of antibodies after infection followed by vaccination, the roles of T cells, and whether booster vaccinations are needed. Hosts: Vincent Racaniello , Rich Condit , Kathy Spindler , and Brianne Barker Guest: Shane Crotty Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode ASV Vaccine Town Halls SARS-CoV-2...
Sep 09, 2021•1 hr 47 min•Ep. 802
The meteorologicomaniacs discuss the COVID-19 pandemic global death toll, effectiveness of vaccines against the delta variant, and how analysis of ancient viral DNA reveals that two pathogenic human viruses were brought into Mexico during the transatlantic slave trade. Hosts: Vincent Racaniello , Alan Dove , Dickson Despommier , Rich Condit , and Brianne Barker Subscribe (free): iTunes , Google Podcasts , RSS , email Become a patron of TWiV! Links for this episode ASV Vaccine Town Halls COVID-19...
Sep 05, 2021•1 hr 57 min•Ep. 801
