You’re listening to the August episode of 3 Minute 3Rs. The papers behind the pod: 1. The ARRIVE guidelines 2.0: Updated guidelines for reporting animal research. PloS Biology https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3000410 2. Acute and chronic stress prevents responses to pain in zebrafish: evidence for stress-induced analgesia. Journal of Experimental Biology . https://jeb.biologists.org/content/223/14/jeb224527 3. 3Rs-based optimization of mice behavioral testing...
Aug 20, 2020•4 min•Ep. 8
You’re listening to the July episode of 3 Minute 3Rs. The papers behind the pod: 1. Reproducibility of animal research in light of biological variation. Nature review Neuroscience https://www.nature.com/articles/s41583-020-0313-3 2. Inactivated rabies vaccines: Standardization of an in vitro assay for residual viable virus detection. Plos Neglected Tropical Diseases https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0008142 3. A novel biosafety level 2 compliant tuberculosis infe...
Jul 16, 2020•4 min•Ep. 7
You’re listening to the June episode of 3 Minute 3Rs. The papers behind the pod: A novel, high-welfare methodology for evaluating poultry red mite interventions in vivo. https://www.sciencedirect.com/science/article/pii/S030440171930041X Pluripotent state transitions coordinate morphogenesis in mouse and human embryos. https://www.nature.com/articles/nature24675 Transcript It’s the Third Thursday of June and you’re listening to 3 Minute 3Rs, your monthly recap of efforts to replace, reduce, and ...
Jun 18, 2020•3 min•Ep. 6
You’re listening to the May episode of 3 Minute 3Rs. The papers behind the pod: 1 Refining rodent models of spinal cord injury: https://doi.org/10.1016/j.expneurol.2020.113273 2 Preclinical validation of the micropipette-guided drug administration (MDA) method in the maternal immune activation model of neurodevelopmental disorders https://www.sciencedirect.com/science/article/pii/S0889159120302543 3 Identifying obstacles preventing the uptake of tunnel handling methods for laboratory mice: An in...
May 21, 2020•4 min•Ep. 5
You’re listening to the April episode of 3 Minute 3Rs, brought to you by the NC3Rs, North American 3Rs Collaborative, and Lab Animal. The papers behind the pod: 1. In Vitro Antiviral Activity and Projection of Optimized Dosing Design of Hydroxychloroquine for the Treatment of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) ( https://www.ncbi.nlm.nih.gov/pubmed/32150618 ) 2. Engineering a 3D functional human peripheral nerve in vitro using the Nerve-on-a-Chip platform. https://www.na...
Apr 16, 2020•4 min•Ep. 4
The March 2020 episode of 3 Minute 3Rs, brought to you by the NC3Rs (www.nc3rs.org.uk), the North American 3Rs Collaborative (www.na3rsc.org), and Lab Animal (www.nature.com/laban)The papers:1. https://pubmed.ncbi.nlm.nih.gov/320766332. https://www.sciencedirect.com/science/article/pii/S02732300203005073. https://www.nature.com/articles/s41598-020-59012-4Transcript[NC3Rs] Nanomedicines have many potential advantages, including their ability to home to specific diseased tissues and exert a locali...
Mar 20, 2020•4 min
The February 2020 episode of 3 Minute 3Rs, brought to you by the NC3Rs (www.nc3rs.org.uk), the North American 3Rs Collaborative (www.na3rsc.org), and Lab Animal (www.nature.com/laban).1. https://www.sciencedirect.com/science/article/pii/S00928674193132362. https://www.sciencedirect.com/science/article/pii/S01650270193030973. https://www.ncbi.nlm.nih.gov/pubmed/31852563[NC3Rs] If you are bitten by a snake today, your anti venom treatment would be largely similar to if you were bitten 100 years ag...
Feb 24, 2020•4 min
The January 2020 episode of 3 Minute 3Rs, brought to you by the NC3Rs (www.nc3rs.org.uk), the North American 3Rs Collaborative (www.na3rsc.org), and Lab Animal (www.nature.com/laban).Papers:1. https://doi.org/10.15252/embr.2019497092. https://www.nature.com/articles/s41598-019-56860-73. https://doi.org/10.1177/0023677219879455[NC3Rs] Academic scientists produce many of the clever ideas which end up as medicines. However, the results in the papers reporting the work are almost always positive, an...
Jan 16, 2020•4 min
The December episode of 3 Minute 3Rs, brought to you by the NC3Rs (www.nc3rs.org.uk), the North American 3Rs Collaborative (www.na3rsc.org), and Lab Animal (www.nature.com/laban).Papers: 1. Cage aggression in group-housed laboratory male mice: an international data crowdsourcing project. https://www.nature.com/articles/s41598-019-51674-z2. Towards animal-free neurotoxicity screening: Applicability of hiPSC-derived neuronal models for in vitro seizure liability assessmenthttps://www.altex.org/ind...
Dec 19, 2019•4 min
The November episode of 3 Minute 3rs, brought to you by the NC3Rs (www.nc3rs.org.uk), the North American 3Rs Collaborative (www.na3rsc.org), and Lab Animal (www.nature.com/laban).Papers:1. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6662399/2. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5371849/3. https://pubs.rsc.org/en/content/articlelanding/2019/LC/C9LC00160C [NA3RsC] Evaluation of developmental and reproductive toxicity is a key aspect of drug development. Each year, 5-7% of newborns world...
Nov 21, 2019•5 min
The October episode of 3 Minute 3rs, brought to you by the NC3Rs (www.nc3rs.org.uk), the North American 3Rs Collaborative (www.na3rsc.org), and Lab Animal (www.nature.com/laban).Papers1. Modelling human hepato-biliary-pancreatic organogenesis from the foregut–midgut boundaryhttps://www.nature.com/articles/s41586-019-1598-02. Automated Platform for Long-Term Culture and High-Content Phenotyping of Single C. elegans Worms https://www.nature.com/articles/s41598-019-50920-83. Analysis of vertebrate ...
Oct 17, 2019•4 min
The September episode of 3 Minute 3rs, brought to you by the NC3Rs (www.nc3rs.org.uk), the North American 3Rs Collaborative (www.na3rsc.org), and Lab Animal (www.nature.com/laban)Papers behind the pod:1. http://jpet.aspetjournals.org/content/371/1/15.long2. https://www.sciencedirect.com/science/article/pii/S001216061830006X3. https://www.nature.com/articles/s41467-019-11259-w[NC3Rs] Accurate measurements of drug and metabolite concentrations in the blood are vital to estimate exposure to the tar...
Sep 19, 2019•4 min
The August episode of 3-Minute 3Rs.Papers:1. https://advances.sciencemag.org/content/5/7/eaaw40992. https://www.ncbi.nlm.nih.gov/pubmed/310260403. https://www.ncbi.nlm.nih.gov/pubmed/31343844[LA] Nociception is the sense that tells animals they are encountering a potentially damaging stimulus. In people, we call it pain. Most pain research is done with rodents, but nociception is pretty well conserved beyond mammals. That includes invertebrates, such as the fruit fly. Some work has been done in ...
Aug 15, 2019•5 min
The July episode of 3-Minute 3Rs from the North American 3Rs Collaborative (www.na3rsc.org), the NC3Rs (www.nc3rs.org.uk), and Lab Animal (www.nature.com/laban)Papers; 1. https://advances.sciencemag.org/content/5/5/eaav65282. https://www.nature.com/articles/s41598-019-44705-23. https://www.nature.com/articles/s41598-019-45464-w[NC3Rs] Colorectal cancer is particularly hard to treat, and only 11% of patients whose tumours have metastasised survive five years after diagnosis. Mouse models that inc...
Jul 18, 2019•5 min
The June episode of 3-Minute 3Rs from the North American 3Rs Collaborative (www.na3rsc.org), the NC3Rs (www.nc3rs.org.uk), and Lab Animal (www.nature.com/laban)Papers; 1. https://bit.ly/2UH4hZt2. https://go.nature.com/2W5CIea3. https://bit.ly/2Fi4td4[LA] On the road to the clinic, immunocompromised mice transplanted with patient-derived xenografts have been a frequent step for testing a drug’s efficacy and safety. However, there may soon be fisher options afoot that might help replace some of th...
Jun 20, 2019•5 min
The May episode of 3-Minute 3Rs from the North American 3Rs Collaborative (www.na3rsc.org), the NC3Rs (www.nc3rs.org.uk), and Lab Animal (www.nature.com/laban)Papers:1. https://bit.ly/2vYuUPR2. https://go.nature.com/2w2tzre3. https://go.nature.com/2VEjEHz[NA3RC] Early mortality of pups in breeding colonies can reach upwards of 50% or higher depending upon the strain. While high pup mortality is often considered "normal", it instead may be indicative of an underestimated animal welfare issue that...
May 16, 2019•5 min
This is the April episode of 3-Minute 3Rs, brought to you by the North American 3Rs Collaborative (www.na3rsc.org), the NC3Rs (www.nc3rs.org.uk), and Lab Animal (www.nature.com/laban)Source papers:1. Characterizing Sources of Variability in Zebrafish Embryo Screening Protocols. https://bit.ly/2VP8njP2. Effective chair training methods for neuroscience research involving rhesus macaques. https://bit.ly/2V7NOSv3. AutonoMouse. https://bit.ly/2VP8tbb. Video: https://www.youtube.com/watch?v=6d7ctvj95...
Apr 18, 2019•5 min
This is the March episode of 3-Minute 3Rs, brought to you by the North American 3Rs Collaborative (www.na3rsc.org, the NC3Rs (www.nc3rs.org.uk), and Lab Animal (www.nature.com/laban)The papers behind the pod:1. Progressive Motor Neuron Pathology and the Role of Astrocytes in a Human Stem Cell Model of VCP-Related ALS. https://bit.ly/2uiF64X2. A critical evaluation of TRPA1-mediated locomotor behavior in zebrafish as a screening tool for novel anti-nociceptive drug discovery. https://go.nature.co...
Mar 21, 2019•4 min
This is the February episode of 3-Minute 3Rs, brought to you by the North American 3Rs Collaborative (www.na3rsc.org, the NC3Rs (www.nc3rs.org.uk), and Lab Animal (www.nature.com/laban)The papers behind the pod:1. 3D-printed Wash Station with Integrated Anesthesia Delivery Manifold for High-throughput Depilation of Laboratory Mice. https://bit.ly/2IpGrAP2. DeepSqueak: a deep learning-based system for detection and analysis of ultrasonic vocalizations .https://go.nature.com/2tupSJK3. Super-Mendel...
Feb 21, 2019•4 min
This is the January episode of 3-Minute 3Rs, brought to you by the North American 3Rs Collaborative (www.na3rsc.org, the NC3Rs (www.nc3rs.org.uk), and Lab Animal (www.nature.com/laban)The papers behind the pod:1. An Atypical Parvovirus Drives Chronic Tubulointerstitial Nephropathy and Kidney Fibrosis https://bit.ly/2FA6dPA2. Remote Welfare Monitoring of Rodents Using Thermal Imaging https://bit.ly/2suGlgm3. No experimental evidence of stress-induced hyperthermia in zebrafish (Danio rerio)https:/...
Jan 17, 2019•4 min
It's the December episode of 3-Minute 3Rs, brought to you by , the NC3Rs (www.nc3rs.org.uk), the North American 3Rs Collaborative (www.na3rsc.org) and Lab Animal (www.nature.com/laban)The papers behind the pod:1. Optimizing the design of population-based patient-derived tumor xenograft studies to better predict clinical response https://bit.ly/2GwGJ7N2. An In Vitro Model of Hematotoxicity https://bit.ly/2Ezgaw33. Found In Translation https://go.nature.com/2UVJ0g6[NC3Rs] Every cancerous tumor is ...
Dec 20, 2018•4 min
It's the November episode of 3-Minute 3Rs, brought to you by Lab Animal (www.nature.com/laban), the NC3Rs (www.nc3rs.org.uk) & the North American 3Rs Collaborative (www.na3rsc.org)The papers behind the pod:1. Sixteen diverse laboratory mouse reference genomes define strain-specific haplotypes and novel functional loci. https://www.nature.com/articles/s41588-018-0223-82. Effects of Cage Enrichment on Behavior, Welfare and Outcome Variability in Female Mice https://www.frontiersin.org/articles...
Nov 15, 2018•4 min
Welcome to the October episode of 3-Minute 3Rs, brought to you by Lab Animal (www.nature.com/laban, the NC3Rs (www.nc3rs.org.uk) & the North American 3Rs Collaborative (www.na3rsc.org)Here are the papers behind the pod:1. Generation of Tumor-Reactive T Cells by Co-culture of Peripheral Blood Lymphocytes and Tumor Organoids. https://www.cell.com/cell/fulltext/S0092-8674(18)30903-62. Mymou: A low-cost, wireless touchscreen system for automated training of nonhuman primates. https://link.spring...
Oct 18, 2018•4 min
Welcome to the September episode of 3-Minute 3Rs, brought to you by Lab Animal (www.nature.com/laban), the NC3Rs (www.nc3rs.org.uk) & the North American 3Rs Collaborative (www.na3rsc.org)Here are the papers behind the pod:1. Assessing animal affect: an automated and self-initiated judgment bias task based on natural investigative behaviour. https://doi.org/10.1038/s41598-018-30571-x2. Comparison of serial blood collection by facial vein and retrobulbar methods in C57BL/6 mice. https://doi.or...
Sep 20, 2018•4 min
You're listening to the August edition of 3 Minute 3Rs, brought to you this month by the NC3Rs (www.nc3rs.org.uk), the North American 3Rs Collaborative (www.na3rsc.org), and Lab Animal (www.nature.com/laban) This month, we'll be talking about non-primate alternatives to study polio, aging & frailty indexes, and training rats with out water restriction.The papers behind the pod:* Replacement of primary monkey kidney cells by L20B cell line in the test for effective inactivation of inactivated...
Aug 16, 2018•4 min
You're listening to the July edition of 3 Minute 3Rs, brought to you this month by the North American 3Rs Collaborative (www.na3rsc.org), Lab Animal (www.nature.com/laban) and the NC3Rs (www.nc3rs.org.uk). This month, we'll be talking about refining mouse anesthesia, replacing mice with zebrafish for studying human hematopoetic stem cells, and validating genome-edited mouse lines.The papers behind the pod:* Effects of Ketamine Compared with Urethane Anesthesia on Vestibular Sensory Evoked Potent...
Jul 19, 2018•4 min
You're listening to the June edition of 3 Minute 3Rs, brought to you this month by Lab Animal (www.nature.com/laban), the NC3Rs (www.nc3rs.org.uk) and the North American 3Rs Collaborative (www.na3rsc.org). We're highlighting Drosophila as a microbiome model, organotypic brain culture slices for Alzheimer's research, and anesthesia for African clawed frogs.The papers behind the pod:* The Drosophila model for microbiome research: https://www.nature.com/articles/s41684-018-0065-0* Preparation of or...
Jun 21, 2018•4 min
Welcome to 3-minute 3Rs for May 2018! in this podcast, we're discussing enrichment, anesthesia for cephalopods, and reproducibility across labs.The papers behind the pod:Laboratory mouse housing conditions can be improved using common environmental enrichment without compromising data: http://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.2005019In Vivo Recording of Neural and Behavioral Correlates of Anesthesia Induction, Reversal, and Euthanasia in Cephalopod Molluscs: https://w...
May 17, 2018•5 min
Welcome to 3-minute 3Rs! In this podcast, we're discussing 'dirty' mice, automating pain assessments, and refining endpoints with telemetry data.The papers behind the pod:News Feature: What happens when lab animals go wild: http://www.pnas.org/content/115/13/3196A deep neural network to assess spontaneous pain from mouse facial expressions: http://journals.sagepub.com/doi/10.1177/1744806918763658Using Telemetry Data to Refine Endpoints for New Zealand White Rabbits Challenged with Bacillus anthr...
Apr 19, 2018•5 min
In this month's podcast, we're highlighting the papers recognized by the NC3Rs International Prize, with topics including in silico modeling to predict cardiac drug toxicity, a novel experimental design to reduce variability, and affimers as an alternative to antibodiesRead more about the Prize and the research here: https://www.nc3rs.org.uk/3rsprizewinners Hosted on Acast. See acast.com/privacy for more information.
Mar 22, 2018•4 min
