Host: Vincent Racaniello Guest: Peter Palese Vincent speaks with Peter Palese about his illustrious career in virology, from early work on neuraminidases to universal influenza virus vaccines. Become a patron of TWiV! Links for this episode Palese Laboratory Pig kidney neuraminidase (Hoppe Seylers Z Physiol Chem) DNAse in cytoplasmic DNA virus (Virology) Inhibitor of influenza virus neuraminidase (Virology) Influenza neuraminidase defective mutants (Virology) Swine influenza virus of 1976 RNA pa...
Jul 03, 2016•1 hr 14 min
Hosts: Vincent Racaniello, Rich Condit , and Kathy Spindler Guest: Stephen J. Russell From ASV 2016 at Virginia Tech, Vincent, Rich and Kathy speak with Stephen Russell about his career and his work on oncolytic virotherapy - using viruses to treat cancers. Become a patron of TWiV! Links for this episode Hokies go viral ( TWiV 285 ) MV NIS de Myelo ( TWiV 298 ) Remission of disseminated myeloma after MV treatment (Mayo Clin Proc) miRNA detargeting (J Virol) Video of this episode at YouTube This ...
Jun 26, 2016•1 hr 1 min
Hosts: Vincent Racaniello and Alan Dove Guest: Erica Ollmann Saphire Vincent and Alan speak with Erica about her career and her work on understanding the functions of proteins of Ebolaviruses, Marburg virus, and other hemorrhagic fever viruses, at ASM Microbe 2016 in Boston, MA. Become a patron of TWiV! Links for this episode Structure of antibodies bound to Ebola virus glycoprotein (PNAS) Structure of cross-reactive filovirus antibody (Cell) Multiple functions of Ebola virus VP40 protein (Cell)...
Jun 19, 2016•1 hr 29 min
Hosts: Vincent Racaniello , Alan Dove , Rich Condit , and Kathy Spindler The TWiVerati revisit possible sexual transmission of Zika virus, and reveal how a cell protein that allows hepatitis C virus replication in cell culture enhances vitamin E mediated protection against lipid peroxidation. Become a patron of TWiV! Links for this episode Late sexual transmission of Zika virus (Lancet) Zika virus disease in US (CDC) Zika virus lab accident (ABC News) Mosquito eradication (virology blog) Florida...
Jun 12, 2016•1 hr 34 min
Host: Vincent Racaniello Guests: Michael Diamond , Michaela Gack , Laura Kramer , and Charles Rice Four virologists discuss our current understanding of Zika virus biology, pathogenesis, transmission, and prevention, in this special live episode recorded at the American Society for Microbiology in Washington, DC. [powerpress url="http://traffic.libsyn.com/twiv/TWiV392.mp3"] Click arrow to play Download TWiV 392 (62 MB .mp3, 85 min) Subscribe (free): iTunes , RSS , email , Google Play Music Video...
Jun 05, 2016•1 hr 25 min
Hosts: Vincent Racaniello , Alan Dove , and Rich Condit Guests: David Pride and Forest Rohwer If you have always wanted to know what coral reefs and the human oral cavity have in common, listen as guests David Pride and Forest Rohwer talk about their work on the microbiomes and viromes of these two environments, and you'll also understand why mucus is cool. Links for this episode Lytic to temperate switching of viral communities (Nature) Viromes in ancestral holobiont Hydra (PLoS One) Phage in h...
May 29, 2016•1 hr 5 min
Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Rich Condit , and Kathy Spindler Guests: Ethan Jackson and Jonathan Carlson Project Premonition, a Microsoft Research project that uses drones to capture mosquitoes and analyze them for pathogens, preprint servers, and three mouse models for Zika virus induced birth defects are the topics of this episode hosted by the TWiumvirate and theirs guests Ethan and Jonathan. Links for this episode Project Premonition Premonition feasibility st...
May 22, 2016•2 hr 14 min
Host: Vincent Racaniello Guest: Stan Lemon Vincent speaks with Stan Lemon about his career in virology, from early work on Epstein Barr virus, through making essential discoveries about hepatitis A virus, hepatitis C virus, and rhinoviruses. Links for this episode Live in Edmonton, Eh? ( TWiV 235 ) Replication of EBV in epithelial cells (Nature) Returning to Carolina after 14 years Evolutionary origins of hepatitis A virus (TWiV 265) cre in rhinovirus genome (RNA) Naked viruses that aren't alway...
May 15, 2016•1 hr 13 min
Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , and Kathy Spindler Preprint servers, the structure of an antibody bound to Zika virus, blocking Zika virus replication in mosquitoes with Wolbachia , and killing carp in Australia with a herpesvirus are topics of this episode hosted by Vincent, Dickson, Alan, and Kathy. Links for this episode biorXiv preprint server Please review Vaccines and Your Child Structure of antibody bound to Zika virus E protein (Cell Host Microbe) Wolbachia b...
May 08, 2016•1 hr 41 min
Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , and Kathy Spindler Guest: Nina Martin Nina Martin joins the TWiV team to talk about the movie Vaxxed, her bout with dengue fever, and the latest research on Zika virus. Links for this episode Public Health United Fifty Shades of Science (TEDx) Parents convicted in death of toddler (Science Based Medicine) CDC: Zika virus causes microcephaly (NEJM) Dengue virus antibodies enhance Zika virus infection (biorXiv) Zika virus in Brazilian no...
May 01, 2016•1 hr 36 min
Hosts: Vincent Racaniello , Rich Condit , and Kathy Spindler Guests: Ted Diehl and Welkin Johnson Ted and Welkin inform the TWiV team how the evolution of ancient retroviruses can be inferred by studying their sequences in the genomes of modern mammals, and join in a discussion of virus dispersal during different methods for drying hands. Links for this episode More fun than a monkey full of viruses ( TWiV 122 ) Tracking ancient retrovirus evolution in mammals (eLife) Virus dispersal during hand...
Apr 24, 2016•1 hr 59 min
Hosts: Vincent Racaniello and Dickson Despommier Guest: Stuart Firestein Stuart joins Vincent and Dickson to talk about his sequel to Ignorance , a book called Failure , which seeks to make science more appealing by revealing its faults. Links for this episode Stuart Firestein's site Ignorance with Stuart Firestein (TWiV Special) The pursuit of ignorance (TED) Ignorance by Stuart Firestein Failure by Stuart Firestein This episode is sponsored by ASM Agar Art Contest and ASV 2016 Send your virolo...
Apr 17, 2016•1 hr 55 min
Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove and Rich Condit Guests: Nischay Mishra and Ian Lipkin Nischay and Ian join the TWiVions to discuss mass die-offs of tilapia by a novel orthomyxo-like virus, Ian's editorial on the movie Vaxxed , and new vaccines to prevent dengue virus infections, including a human challenge model. Links for this episode Novel orthomyxo-like virus in Tilapia die-offs (mBio) Fish flu ( TWiV 41 ) Anti-vaccination lunacy won't stop (WSJ) Single-dose dengue ...
Apr 10, 2016•1 hr 45 min
Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove and Kathy Spindler Guest: Esper Kallas Esper and the Merry TWiXters analyze the latest data on Zika virus and microcephaly in Brazil, and discuss publications on a mouse model for disease, infection of a fetus, mosquito vector competence, and the cryo-EM structure of the virus particle. Links for this episode Zika virus in the Americas (Science) Co-circulation of dengue, Zika, chik in Brazil (Medicine) Murine model for Zika virus infecti...
Apr 03, 2016•2 hr 5 min
Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove and Kathy Spindler Guests: Nels Elde and Edward Chuong Nels and Ed join the TWiV team to talk about their observation that regulation of the human interferon response depends on regulatory sequences that were co-opted millions of years ago from endogenous retroviruses. Links for this episode Endogenous retroviruses and regulation of innate immunity (Science) Placenta is viral (Nature) An ERV that maintains pluripotency of human embryonic...
Mar 27, 2016•1 hr 54 min
Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Rich Condit , and Kathy Spindler Guest: Carl Zimmer Carl Zimmer joins the TWiV team to talk about his career in science writing, the real meaning of copy-paste, science publishing, the value of Twitter, preprint servers, his thoughts on science outreach, and much more. Links for this episode Carlzimmer.com All Carl's articles at NYTimes Carl at Stat The Loom Staying afloat in the rising tide of science (Cell) Parasite Rex Planet of Vir...
Mar 20, 2016•1 hr 59 min
Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Rich Condit , and Kathy Spindler The TWiVeroos deliver the weekly Zika Report, then talk about a cryoEM structure of a plant virus that reveals how the RNA genome is packaged in the capsid, and MIMIVIRE, a CRISPR-like defense system in giant eukaryotic viruses. Links for this episode First worms, then viruses, now dormant cyber pathogens (Zdziarski blog) Guillain-Barré with Zika virus in urine (Eurosurveillance) Zika virus in urine , B...
Mar 13, 2016•1 hr 50 min
Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Rich Condit , and Kathy Spindler Guest: Scott Tibbetts Scott Tibbetts joins the TWiVists to describe his work on the role of a herpesviral nocoding RNA in establishment of peripheral latency, and then we visit two last minute additions to the Zika virus literature. Links for this episode Noncoding RNA required for establishment of latency (mSphere) 17:20 Lessons learned from in vivo studies of a viral noncoding RNA (mSphere) 1:05:05 Zi...
Mar 06, 2016•1 hr 55 min
Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , and Kathy Spindler Guests: Greg Smith and Mark Fuccio Greg Smith joins the TWiVirate to reveal how his lab discovered a switch that controls herpesvirus neuroinvasion, and then we visit the week's news about Zika virus. Links for this episode Dynamic ubiquitination drives herpesvirus neuroinvasion (PNAS) Releasing Zika virus data in real time (Nature) Zika virus infection and stillbirths (PLoS NTD) Zika virus infection, imported , Ital...
Feb 28, 2016•1 hr 59 min
Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Rich Condit , and Kathy Spindler The TWiVniks review the past week's findings on Zika virus and microcephaly, and reveal a chicken protein that provides insight on the restriction of transmission of avian influenza viruses to humans. Links for this episode Larvicide not involved in microcephaly (CBS News) Obama seeks $1.8b for Zika virus (Guardian) Zika virus in Puerto Rico (MMWR) Zika virus in semen (EID) Zika virus in Indonesia (EID)...
Feb 21, 2016•1 hr 35 min
Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Rich Condit , and Kathy Spindler Guest: Jeremy Luban The TWiV team discusses the latest data on Zika virus, including ocular defects in infants with microcephaly, and isolation of the entire viral genome from fetal brain tissue. Links for this episode Ocular findings in infants with microcephaly ( JAMA and Arq Bras Oft ) Evidence of Zika virus infection in brain and placenta (MMWR) Zika virus associated with microcephaly (NEJM) Hard-co...
Feb 14, 2016•1 hr 49 min
Hosts: Vincent Racaniello , Alan Dove , Rich Condit , and Kathy Spindler Guests: Carolyn Coyne , Helen Lazear , and Jeremy Luban The TWiVziks present everything you want to know about Zika virus, including association of infection with microcephaly and Guillain-Barré syndrome, transmission, epidemiology, and much more. Links for this episode Zika outbreak in Brazil (EID) 13:40 Zika summary (ProMedMail) 19:30 Zika and microcephaly (MMWR) 27:30 Facts about microcephaly (CDC) Brazil Ministry of Hea...
Feb 07, 2016•2 hr 5 min
Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Rich Condit , and Kathy Spindler The TWiVniks consider the role of a cell enzyme that removes a protein linked to the 5'-end of the picornavirus genome, and the connection between malaria, Epstein-Barr virus, and endemic Burkitt's lymphoma. Links for this episode Divergent requirements for removing VPg (mBio) Bond, covalent bond ( TWiV 210 ) Link between malaria and endemic Burkitt's lymphoma (PLoS Path) Multifactorial role of malaria ...
Jan 31, 2016•1 hr 54 min
Host: Vincent Racaniello Guest: Julius S. Youngner Vincent speaks with Julius about his long career in virology, including his crucial work as part of the team at the University of Pittsburgh that developed the Salk inactivated poliovirus vaccine. Links for this episode Trypsin for cell cultures (Proc Soc Exp Biol Med) Interferon induction by nonviral stimuli (J Gen Physiol) Interferons of different molecular weight (Proc Soc Exp Biol Med) Poliovirus thermal mutants (J Bacteriol) Combination of ...
Jan 24, 2016•1 hr 20 min
Hosts: Vincent Racaniello , Alan Dove , Rich Condit , and Kathy Spindler The TWiV-osphere introduces influenza D virus, virus-like particles encoded in the wasp genome which protect its eggs from caterpillar immunity, and a cytomegalovirus protein counters a host restriction protein that prevents establishment of latency. Links for this episode Ebolavirus update (ProMedMail) Influenza D virus (mBio) Vincent's influenza C virus paper (J Virol) Virus-like particles protect wasp eggs (Sci Adv) CMV ...
Jan 17, 2016•2 hr 5 min
Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Rich Condit , and Kathy Spindler The TWiVologists discuss the finding of a second transmissible cancer in Tasmanian devils, and development of new poliovirus strains for the production of inactivated vaccine in the post-eradication era. Links for this episode Second transmissible cancer in Tasmanian Devils (PNAS) New strains for production of IPV (PLoS Path) Synchronized switch to bivalent OPV (polioeradication.org) Virologists, start ...
Jan 10, 2016•2 hr 10 min
Hosts: Vincent Racaniello , Alan Dove , and Kathy Spindler The TWiVomics review ten captivating virology stories from 2015. Ten virology stories from 2015 Recoding to make vaccines and study viral functions: TWiV 351: The dengue code TWiV 362: Gotta catch ’em all and n ew paper Defining the virome: TWiV 365: Blood, feuds, and a foodborne disease , TWiV 362: Gotta catch ’em all , TWiV 356: Got viruses? , TWiV 355: Baby’s first virome , TWiV 342: Public epitope #1 , TWiV 323: A skid loader full of...
Jan 03, 2016•1 hr 49 min
Hosts: Vincent Racaniello , Alan Dove , and Kathy Spindler A swarm of virologists discusses testing of a MERS coronavirus vaccine for camels, and how a neuronal stress pathway reactivates herpes simplex virus. Links for this episode MERS coronavirus vaccine for camels (Science) WHO MERS summary Neuronal stress pathway and herpes simplex virus reactivation (Cell Host Micr) Stress flips a chromatin switch (Cell Host Micr) How herpes simplex virus reactivates (UNC press release) Histone antibodies ...
Dec 27, 2015•1 hr 58 min
Hosts: Vincent Racaniello , Alan Dove , Rich Condit , and Kathy Spindler A plaque of virologists explores the biology of Zika virus and recent outbreaks, and the contribution of a filamentous bacteriophage to the development of biofilms. Links for this episode More cowbell (Wikipedia) Microcephaly in Brazil (Outbreak News) Non-vector borne Zika transmission (EID) Zika virus outside Africa (EID) Zika virus possible sexual transmission (EID) Zika virus in saliva (J Clin Virol) Dengue's cousin Zika...
Dec 20, 2015•1 hr 59 min
Hosts: Vincent Racaniello , Dickson Despommier, Alan Dove , Rich Condit, and Kathy Spindler Guests: Carolyn Coyne and Coyne Drummond Two Coynes join the TWiV overlords to explain their three-dimensional culture model of polarized intestinal cells for studying enterovirus infection. Links for this episode Carolyn and placentas on TWiV 193 Three-dimensional cell culture model (mSphere) Bioreactor video (JoVE) Neurovirologist Richard T. Johnson, 84 ( Hopkins and virology blog ) Tardigrade HGT dispu...
Dec 13, 2015•1 hr 49 min
