Host: Vincent Racaniello Guests: Carla Giles, Zoe Dyson, Brianna McLean, and Caitlin O'Brien In Melbourne, Australia, Vincent speaks with four PhD students about their research projects and what it's like to get a doctorate down under. Video of this episode: view at YouTube This episode is sponsored by ASM Microbe and ASM Biodefense . Send your virology questions and comments to twiv@twiv.tv TWiV is a MicrobeTV Production...
Hosts: Vincent Racaniello , Alan Dove , and Kathy Spindler For a TWiV Thanksgiving, Vincent, Alan, and Kathy trace the feud over genome editing, a new virus discovered in human blood, and the origins of hepatitis A virus. Links for this episode Battle over genome editing (Wired) 10:05 First description of CRISPR in E. coli (J Bacteriol) 14:00 Cas genes identified (Mol Micro) 14:20 CRISPR-Cas provides immunity to bacterial viruses (Science) 15:35 Cas9-crRNA complex mediates DNA cleavage (PNAS) 16...
Hosts: Vincent Racaniello , Rich Condit , and Kathy Spindler Guests: Ralph Baric and Vineet Menachery Vincent, Rich, and Kathy speak with Ralph and Vineet about their research on the potential of SARS-like bat coronaviruses to infect human cells and cause disease in mice. Links for this episode Zoonotic potential of SARS-like bat viruses (Nature) SARS-CoV susceptibility loci via collaborative cross (PLoS Genetics) 47:00, 57:25 Ebright and Wain-Hobson quotes (Nature) 1:17:20 Misleading press rele...
Hosts: Vincent Racaniello , Alan Dove , Rich Condit , and Kathy Spindler The TWiVers reveal influenza virus replication in the ferret mammary gland and spread to a nursing infant, and selection of transmissible influenza viruses in the soft palate. Links for this episode Photos of my visit to OHSU (Facebook) 6:30 Folta departs (Facebook) 9:25 T-VEC approved for melanoma (FDA) 14:45 Talimogene laherparepvec (Wikipedia) 15:10 T-VEC phase III trial (J Clin Oncol) 17:35 How drugs are named (PopSci) ...
Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Rich Condit , and Kathy Spindler With their usual verve, the virus virtuosos illuminate a new method to identify all the viral nucleic acids in a sample, and regulation of viral gene expression by codon usage. Links for this episode Virome capture sequencing (mBio) 15:40 Codon usage regulates viral gene expression (PNAS) 40:00 Harold S. Ginsberg (Wikipedia) 7:15 Letters read on TWiV 362 6:50, 1:03:20 This episode is sponsored by ASM Ed...
Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , and Rich Condit The TWiVsters discuss Frederick Novy's return from retirement to recover a lost rat virus, and evidence for persistence of Ebolavirus in semen. Links for this episode Frederick Novy and the rat virus (Ann Int Med) 18:20 The rat virus (J Inf Dis) 22:55 Recovery of the rat virus (J Inf Dis) 35:20 Ebola sitrep 42:25 Meningitis in Glaswegian Ebolavirus nurse ( ProMedMail , Medscape ) 45:50 Sexual transmission of Ebolavirus ...
Hosts: Vincent Racaniello and Kathy Spindler Guests: Michael Imperiale , Adam Lauring , and Akira Ohno Vincent visits the University of Michigan where he and Kathy speak with Michael, Adam, and Akira about polyomaviruses, virus evolution, and virus assembly, on the occasion of naming the department of Microbiology & Immunology a Milestones in Microbiology site. Links for this episode World Polio Day 3:20 Mole Day 5:25 The rat virus (J Inf Dis) 20:00 Polyomavirus miRNAs (Curr Op Virol) 39:30 ...
Host: Vincent Racaniello Guest: Blossom Damania Vincent speaks with Blossom about her laboratory's research on Kaposi's sarcoma-associated herpesvirus, including how it transforms cells, the switch between lytic and latent replication, and its interaction with the innate immune system of the host. Links for this episode Damania Laboratory Modulation of cGAS-STING by KSHV (PNAS) KSHV vIRF1 interacts with ISG15 pathway member (J Virol) KSHV NLR homolog inhibits inflammasome (Science) KSHV inhibiti...
Host: Vincent Racaniello Guest: Ileana Cristea Vincent meets up with Ileana at Princeton University to talk about how her laboratory integrates molecular virology, mass spectrometry-based proteomics, and bioinformatics to unravel the interplay between virus and host. Links for this episode Cristea Laboratory Ileana's work previously on TWiV 269 Blowing off steam (Cell Host Micr) Nuclear viral DNA sensors (J Biol Chem) mSystems Video of this episode - view at YouTube (coming soon!) Send your viro...
Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Rich Condit , and Kathy Spindler Guest: Jens Kuhn Jens joins the TWiVomics to discuss the strengths and weaknesses of viral taxonomy, including its history and evolution, how viruses are ordered, and why T. rex was classified without having a living isolate. Links for this episode Jens' previous TWiV (283) 5:15 International Committee on the Taxonomy of Viruses (ICTV) 27:50 ICTV 9th Report 32:55 Classifying viruses by capsid architectu...
Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Rich Condit , and Kathy Spindler Guest: Stephanie Neal Stephanie joins the super professors to discuss the gut virome of children with serious malnutrition, caterpillar genes acquired from parasitic wasps, and the effect of adding chemokines to a simian immunodeficiency virus DNA vaccine. Links for this episode Invitrogen Science Hero Awards 15:05 Gut DNA viromes of twins with severe malnutrition (PNAS) 34:30 Random forests 48:00 Wasps...
Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Rich Condit , and Kathy Spindler The TWiV team considers the effect of a Leishmaniavirus on the efficacy of drug treatment, and the human fecal virome and microbiome in twins during early infancy. Links for this episode Announcing mSphere 5:50 Leishmania virus influences drug outcome ( paper 1 , paper 2 ) 25:15 Human gut virome and microbiome in infants (Nat Med) 46:00 Multiple displacement amplification (Wiki) 46:00 Virome of adult tw...
Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Rich Condit , and Kathy Spindler The esteemed doctors of TWiV review a new giant virus recovered from the Siberian permafrost, why influenza virus gain of function experiments are valuable, and feline immunodeficiency virus. Links for this episode Non-viral proteins in vaccinia virus particles (Arch Virol) 8:15 Thing a week (Jonathan Coulton, downloads ) 23:20 Immortal Life pornographic? Not! (HuffPost) 25:25 Mollivirus sibericum (PNAS...
Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , and Kathy Spindler The TWiVniacs discuss twenty-eight years of poliovirus shedding by an immunodeficient patient, and packaging of the innate cytoplasmic signaling molecule cyclic GMP-AMP in virus particles. Links for this episode 28 years of poliovirus shedding (PLoS Path) 36:50 NOT a rare poliovirus (Wash Post) 44:35 Ukraine polio outbreak (ProMedMail) 41:00 Virus particle transfer of cGAMP (Science one , two ) 47:50 cGAS on TWiV 222...
Host: Vincent Racaniello Guest: Michele Banks Vincent meets up with Michele Banks in Washington, DC to discuss her career as a creator of science-themed art. Links for this episode Michele Banks on Twitter Artologica Michele's blog The Finch and the Pea Joseph Cornell Not Exactly Rocket Science (Ed Yong) Tree of Life (Jonathan Eisen) Home Microbiome Study Kitten Microbiome Project Science Online The Vexed Muddler Luke Jerram A Daily Dish (Klari Reis) Neuroscience art (Greg Dunn) Ai Weiwei Video ...
Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Rich Condit , and Kathy Spindler The Masters of the ScienTWIVic Universe discuss a novel poxvirus isolate from an immunosuppressed patient, H1N1 and the gain-of-function debate, and attenuation of dengue virus by recoding the genome. Links for this episode Paula Pitha-Rowe (Balt Sun) Novel poxvirus (CID) H1N1 and gain-of-function debate (mBio) Recoding dengue virus genome (PNAS) Attenuation by dinucleotide frequency change (PNAS) Codon...
Host: Vincent Racaniello Guest: Katherine A. High Vincent speaks with Katherine High about her career and her work on using viral gene therapy to treat inherited disorders. This episode is drawn from one of twenty-six video interviews with leading scientists who have made significant contributions to the field of virology, part of the new edition of the textbook Principles of Virology . Links for this episode Katherine A. High, MD AAV gene therapy for congenital blindness (Sci Trans Med) Gene th...
Hosts: Vincent Racaniello , Alan Dove , and Rich Condit Vincent, Alan and Rich explain how to make a functional ribosome with tethered subunits, and review the results of a phase III VSV-vectored Ebolavirus vaccine trial in Guinea. Links for this episode Social media specialist position at ASM WHO dismisses Catholic church on vaccine safety U of T Dean resigns over anti-vaccine course (Tor Star) Vaccine Adverse Event Reporting System (VAERS) Countering anti-vaccination attitudes (PNAS) Designer ...
Hosts: Vincent Racaniello and Rich Condit Vincent and Rich discuss fruit fly viruses, one year without polio in Nigeria, and a permissive Marek's disease viral vaccine that allows transmission of virulent viruses. Links for this episode Virology tenure-track position at NCI (pdf) ASM Agar Art Contest Nigeria on brink of polio eradication (Nature) Dengue in Africa (Tyler Sharp) Permissive vaccines and virulence (PLoS Biol) Marek's disease vaccines and virulence (Ed Yong) Image credit Letters read...
Hosts: Vincent Racaniello , Alan Dove , and Rich Condit Vincent, Alan, and Rich discuss the virus behind rose rosette disease, and fatal human encephalitis caused by a variegated squirrel bornavirus. Links for this episode Virology tenure-track position at NIH (pdf) 7:00 Voinnet investigation is a Swiss Federal secret (twitter) 12:25 Rose rosette disease in Ft. Worth (Star Telegram) 29:35 Rose rosette virus discovered (J Gen Virol) 34:25 Emeravirus (ViralZone) 33:55 Eriophyid mites (MI Botanical...
Hosts: Vincent Racaniello , Rich Condit , and Kathy Spindler Guest: Joan Steitz This episode was recorded at the 34th Annual Meeting of the American Society for Virology, where Vincent, Rich, and Kathy spoke with Joan Steitz, a tireless promoter of women in science and one of the greatest scientists of our generation. Links for this episode ASV annual meeting , Western University Steitz Laboratory at Yale University Steitz Laboratory on Twitter Panoramic photo of ASV TWiV by Matt Evans Photos of...
Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , and Kathy Spindler The TWiVonauts review how the weather affects West Nile virus disease in the US, benefit of B cell depletion for ME/CFS patients, and an autoimmune reaction induced by influenza virus vaccine that leads to narcolepsy. Links for this episode Virology faculty position, University of Toledo Weather and West Nile virus disease (AJTMH) B cell depletion benefits ME/CFS patients (PLoS One) Antibodies to influenza nucleoprot...
Host: Vincent Racaniello Guests: Ruth Jarrett , Glen Nemerow , and Esther Schnettler At the Glasgow Science Festival microTALKS, Vincent speaks with Ruth, Glen, and Esther about their research on viruses and Hodgkin lymphoma, adenovirus structure and entry into cells, and interactions between arthropod borne viruses and their hosts. Links for this episode Glasgow Science Festival microTALKS Hodgkin lymphoma (Histopath) HHV-6 and Hodgkin lymphoma (PLoS One) Adenovirus entry (PLoS Path) Adenovirus...
Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Rich Condit , and Kathy Spindler The TWiVerinoes discuss the potential for prion spread by plants, global circulation patterns of influenza virus, and the roles of Argonautes and a viral protein in RNA silencing in plants. Links for this episode Prions in plants (Cell Rep) 11:35 Global influenza virus circulation (Nature) 20:40 Rough patch for plant RNA silencing (Lab Times) 29:50 Role of Argonaute proteins in plant viral defense (PLoS...
Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Rich Condit , and Kathy Spindler The TWiVniks discuss the structure of a virus that reproduces in an extreme environment, long-term consequences of Ebolavirus infection, and VirScan, a method to identify the different virus infections you have had in your lifetime. Links for this episode Virus with A-form DNA (Science) Viruses in the extreme (virology blog) Sequelae of Ebolavirus infection (Lancet Inf Dis) Long term Ebolavirus effects ...
Host: Vincent Racaniello Guests: Adam Kucharski , Gillian Slack , and Emma Thomson Vincent returns to the University of Glasgow MRC-Center for Virus Research and speaks with Emma, Gillian, and Adam about their ebolavirus experiences: caring for an infected patient, working in an Ebola treatment center in Sierra Leone, and making epidemiological predictions about the outbreak in west Africa. Links for this episode Tribute to Richard Elliott Donate to mesothelioma research for Richard Elliott CVR ...
Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Rich Condit , and Kathy Spindler The TWiV teams reviews a MERS-coronavirus serosurvey and an outbreak in South Korea, and constraints on measles virus antigenic variation. Links for this episode Richard Elliott on TWiV 177 MERS-CoV outbreak , South Korea (ProMedMail) MERS-CoV serosurvey (Lancet Inf Dis) Biolabs in your backyard (USA Today) Constraints on measles virus antigenic variation (Cell Rep) Selection bias and bombers (John D. C...
Hosts: Vincent Racaniello , Alan Dove , and Kathy Spindler Tre TWiV amici present three snippets and a side of sashimi: how herpesvirus inhibits host cell gene expression by disrupting transcription termination. Links for this episode Searching for Steamer in the oceans Lassa fever death in NJ ( CDC and NYTimes ) Transgenic mouse model for MERS-CoV (J Virol) First West Nile virus disease case in Texas Herpesvirus disrupts host transcription termination (Nat Comm) Sashimi plots Broad benefits of ...
Hosts: Vincent Racaniello and Kathy Spindler Guest: Laura Newcomb Vincent and Kathy visit Laura Newcomb at California State University San Bernardino, where they talk about Laura's viral tatoos, and a protein that protects the mosquito brain from lethal flavivirus infection. Links for this episode Neuron-specific mosquito antiviral mechanism (PLoS Path) Letters read on TWiV 338 Photos of our visit Video of this episode - view at YouTube Weekly Science Picks Laura - NP siRNA inhibits different in...
Host: Vincent Racaniello Guests: Michael Metzger and Steve Goff Vincent meets up with Michael and Steve to discuss their finding of a transmissible tumor in soft shell clams associated with a retrovirus-like element in the clam genome. Links for this episode Hemocytic leukemia in soft shell clam (Sci Total Environ) Activation of Steamer in neoplastic hemocytes (PNAS) Transmission of clonal cancer cells in clams (Cell) Steve Goff on TWiV #76 Image credit Timestamps by Jennifer . Thank you! Send y...
