Host: Vincent Racaniello Guests: Susan Baker and Thomas Gallagher Vincent meets up with Susan Baker and Tom Gallagher at Loyola University to talk about their work on coronaviruses. Links for this episode Papain-like viral proteases (Virus Res) MERS-CoV papain-like protease (Virology) Mouse model for MERS-CoV (PNAS) Receptor and MERS-CoV infection (J Virol) Letters read on TWiV 276 Video of this episode - view at YouTube Weekly Science Picks Vincent - First demonstration of Macintosh, 1984 Liste...
Mar 16, 2014•1 hr 10 min
Hosts: Vincent Racaniello and Rich Condit Guest: Eugene Koonin Vincent and Rich meet up with Eugene Koonin to talk about the central role of viruses in the evolution of all life. Links for this episode Virocentric view of evolution (Curr Op Virol) Proposed order Megavirales (Arch Virol) Pithovirus (PNAS) Selfish virus-like elements (Virol J) Pandoraviruses are derived Phycodnaviruses (Biol Direct) Video of this episode - view below at YouTube Send your virology questions and comments (email or m...
Mar 09, 2014•2 hr 10 min
Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Rich Condit , and Kathy Spindler The TWiV team discusses recent cases of polio-like paralysis in California, and the virome of 14th century paleofeces. Links for this episode MERS-CoV in dromedary camels (EID) Virologist Al Kapikian dies Polio-like illness in CA (ProMedMail) Acute flaccid paralysis AFP in India (EID) Enterovirus 68 (MMWR) EV 68 CNS infection (Arch Path Lab Med) Polio-free does not mean paralysis-free (The Hindu) Viruse...
Mar 02, 2014•2 hr
Host: Vincent Racaniello Guests: W. Ian Lipkin and Thomas Briese Vincent meets up with Ian and Thomas to discuss their finding that MERS-coronavirus has been circulating in dromedary camels in the Kingdom of Saudi Arabia since at least 1992. Links for this episode MERS-CoV in dromedary camels (mBio) TWiV 251: Don't kiss the camel TWiV 247: Today's weather in virology TWiV 239: Filterable camels Send your virology questions and comments (email or mp3 file) to twiv@twiv.tv...
Feb 25, 2014•38 min
Hosts: Vincent Racaniello , Dickson Despommier , Rich Condit , and Kathy Spindler The TWiVome dissect the finding that interferon lambda alleles predict the outcome of hepatitis C virus infection. Links for this episode Interferon lambda and HCV outcome (Cell Host Micr) Laser capture microdissection (Wikipedia) HCV dependent fluorescent reporter (Nat Biotech) Bats and viruses (PLoS Path) White nose syndrome (PLoS Path) Mouse phenome database Letters read on TWiV 273 Weekly Science Picks Rich - T...
Feb 23, 2014•1 hr 43 min
Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Rich Condit , and Kathy Spindler The TWiV team describes aphid control by using a viral capsid protein to deliver a spider toxin to plants, and a human endogenous retrovirus that enhances expression of a neuronal gene. Links for this episode Toxin delivery to plants by viral capsid protein (Nat Biotech) Pea enation mosaic virus (ViralZone) Image credit: Aphid membrane feeding chamber (Sci Rep) Aphid feeding chamber (YouTube) Parafilm (...
Feb 16, 2014•1 hr 48 min
Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Rich Condit , and Ashlee Bennett The TWiV crew discusses two reports on viruses that might have crossed kingdoms, from plants to honeybees and from plants to vertebrates. Links for this episode Tobacco ringspot virus in honeybees (mBio) Plant virus that switched to vertebrates (PNAS) CDC influenza activity and surveillance With Esmeralda at ASM Biodefense (jpg) Image credit: Virginia Tech Learning Resources Center Letters read on TWiV ...
Feb 09, 2014•1 hr 36 min
Hosts: Vincent Racaniello and Rich Condit Guests: Dennis Hruby and Yoshihiro Kawaoka Vincent and Rich discuss avian influenza virus and an antiviral drug against smallpox with Dennis and Yoshi at the ASM Biodefense and Emerging Diseases Research Meeting in Washington, DC. Links for this episode ASM Biodefense Meeting Influenza H5N1 transmission (Virus Res) Aerosol transmission of H5N1 virus in ferrets (Nature) Characterization of H7N9 virus from humans (Nature) ST-246 efficacy in primates (AAC) ...
Feb 02, 2014•1 hr 24 min
Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Rich Condit , and Kathy Spindler The complete TWiV team reviews evidence for sensing of herpesviral DNA in the nucleus by the cell protein IFI16. Links for this episode IFI16 is a nuclear pathogen sensor (Cell Host Micr) Nuclear IFI16 sensing of and antagonism by herpesvirus (PNAS) Acetylation regulates IFI16 location (PNAS) Letters read on TWiV 269 Weekly Science Picks Kathy - Female conveners and speakers Alan - Sandgrains Rich - 201...
Jan 26, 2014•1 hr 42 min
Hosts: Vincent Racaniello , Alan Dove , Kathy Spindler , and Ashlee Bennett Vincent, Alan, Kathy, and Ashlee discuss fomites in physicians offices, plant virus factories involved in aphid transmission, and clues from the bat genome about flight and immunity. Links for this episode How viruses cause disease (Coursera) How clean is your iPad (Which?) Meeting announcement: From emerging to pandemic viruses CaMV virus factories involved in aphid transmission (J Virol) Caulimovirus (ViralZone) CaMV r...
Jan 19, 2014•1 hr 46 min
Hosts: Vincent Racaniello , Alan Dove , Rich Condit , and Kathy Spindler Vincent, Alan, Rich and Kathy review a protease essential for influenza pathogenesis in mice, and directionality of rhinovirus RNA exit from the capsid. Links for this episode Protease essential for influenza pathogenesis in mice (PLoS Path) Rhinovirus uncoating is directional (PLoS Path) Fluorescence correlation spectroscopy Capillary electrophoresis (Wiki) Letters read on TWiV 267 Weekly Science Picks Kathy - Amazing maps...
Jan 12, 2014•1 hr 35 min
Hosts: Vincent Racaniello , Alan Dove , and Kathy Spindler Vincent, Alan, and Kathy discuss finding viruses in outer space, varying results obtained from personal genetic testing, and depletion of CD4 cells during HIV infection by pyroptosis. Links for this episode We should hunt for viruses in space (New Scientist) I had my DNA picture taken (NY Times) CD4 cell death by pyroptosis (Nature) Abortive HIV infection in lymphoid tissue (Cell) IFI16 sensor required for HIV mediated cell death (Scienc...
Jan 05, 2014•1 hr 37 min
Hosts: Vincent Racaniello , Alan Dove , Rich Condit , and Kathy Spindler In their final episode of the year, the TWiV team reviews ten compelling virology stories from 2013. Links for this episode Antiviral RNAi (TWiV 255 , 256 , 263 ) MERS-CoV (TWiV 215 , 223 , 224 , 233 , 239 , 247 , 251 , 258 ) Placental trophoblasts confer viral resistance (TWiV 241 ) Origin of REV (TWiV 248 ) Phage T7 gets a cat scan (TWiV 220 ) It's a cGAS! (TWiV 222 ) Dual virus-host arms race (TWiV 242 ) Pandoraviruses (...
Dec 29, 2013•1 hr 43 min
Hosts: Vincent Racaniello , Alan Dove , Rich Condit , and Kathy Spindler The TWiVites read listener questions and comments about public engagement in science, vaccines, RNAi, reprogramming CD8 cells to treat cancer, rabies, and much more. Links for this episode Viral gifts Controlling plant viruses by vector control (Ann Rev Phyto) Flavivirus RNAi suppressor (J Virol) Entre probetas podcast (iTunes) Hedgehog central Reprogramming T cells for leukemia therapy (NEJM) Petri Dish podcast On Your Min...
Dec 22, 2013•1 hr 40 min
Hosts: Vincent Racaniello , Dickson Despommier, Alan Dove , Rich Condit , Kathy Spindler , and Ben tenOever Ben joins the TWiV team to reveal the winner of his contest in which influenza viruses carrying different interferon-stimulated genes vie against one another in mice. Links for this episode Virusninja Game of clones (Vax Rep) Game of clones bracket Letters read on TWiV 263 Weekly Science Picks Kathy - The Brain Scoop , esp. Where My Ladies At Alan - Say 'hi' to a spaceship (YouTube)Rich - ...
Dec 15, 2013•1 hr 44 min
Host: Vincent Racaniello Guest: Ann Palmenberg Vincent returns to the University of Wisconsin - Madison to speak with Ann Palmenberg about her career in virology. Links for this episode Palmenberg lab website - great resources Genome sequences of all rhinoviruses (J All Clin Imm) Cardioviral poly(C) tracts (Arch Virol Supp) EMCV disruption of nuclear transport (PNAS) Picornavirus protease in replicase gene (J Virol) Bacteriophage Qbeta replicase (J Virol) Thanks to Jordan for the episode title! ...
Dec 09, 2013•1 hr 36 min
Hosts: Vincent Racaniello and James Van Etten Guests: Chantal Abergel and Jean-Michel Claverie Vincent meets up with Chantal and Jean-Michel at the first International Symposium on Giant Virus Biology in Tegernsee, Germany, to discuss their work on Mimivirus, Megavirus, and Pandoravirus. Links for this episode Unanswered giant virus questions (Adv Virus Res) Fourth domain of life (Commun Integr Biol) Pandoraviruses (Science) Phaeocystis globosa virus (PNAS) Cafeteria roenbergensis virus (PNAS) S...
Dec 01, 2013•1 hr 12 min
Host: Vincent Racaniello Guests: Tom Friedrich , Tony Goldberg , and David O'Connor Vincent visits the University of Wisconsin, Madison and speaks with Tom, Tony, and David about their work on virus discovery at the AIDS Vaccine Research Laboratory. Links for this episode A tick in Tony's nose (Am J Trop Med Hyg) Two new SIV from colobus monkeys in Uganda (Biomed Cent) Pegivirus diversity in Uganda (J Gen Virol) Simian hemorrhagic fever virus diversity in African primates (J Virol) Video of this...
Nov 24, 2013•1 hr 35 min
Hosts: Vincent Racaniello and Rich Condit Guest: Jaquelin Dudley Vincent and Rich join Jackie at the University of Texas, Austin to talk about her work on mouse mammary tumor virus. Links for this episode Rem signal peptide processing and function (J Virol) MMTV null mice and tumorigenesis (J Virol) Jackie's publications (PubMed) I want my MMTV (TWiV 242) Live at ASV in Madison (TWiV 193) Symbiotic safecrackers (TWiV 154) Send your virology questions and comments (email or mp3 file) to twiv@twiv...
Nov 16, 2013•1 hr 27 min
Hosts: Vincent Racaniello , Alan Dove , Rich Condit , Kathy Spindler , and Matt Frieman Matt joins the TWiV team to discuss the discovery of a SARS-like coronavirus in bats that can infect human cells, and what is going on with MERS-coronavirus. Links for this episode S ARS-like virus in bats binds ACE2 (Nature) Bat SARS-like virus that infects human cells (virology blog) Coronavirus infections update (WHO) Lack of MERS coronavirus antibodies in humans (EID) Mice not susceptible to MERS-CoV (J G...
Nov 11, 2013•1 hr 26 min
Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Rich Condit , and Kathy Spindler The TWiV team consider how the kinase mTOR modulates the antibody response to provide broad protection against influenza virus, and explore the problems with scientific research. Links for this episode T wo lives intertwined (Ann Rev Imm) John Holland, 83 Kinase mTOR modulates antibody response (Nat Imm) Drug widens immunity to flu (TheScientist) Concerto in B (TWiV 161) How science goes wrong (Economis...
Nov 03, 2013•1 hr 42 min
Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Rich Condit , and Kathy Spindler Vincent, Dickson, Alan, Rich, and Kathy review two papers that present evidence for RNA interference as an antiviral immunity mechanism in mammals. Links for this episode: World Polio Day Mole Day John Holland's publications RNAi is antiviral in mammals (Science) Antiviral RNA interference in mammals (Science) RNAi, antiviral after all (Science) Is RNA interference antiviral in mammals? (Cell Host Micro...
Oct 27, 2013•1 hr 36 min
Hosts: Vincent Racaniello and Rich Condit Guests: Robert Krug and Christopher Sullivan Vincent and Rich visit the University of Texas at Austin and meet up with Bob and Chris to talk about their work on influenza virus and microRNAs. Links for this episode: Virus-encoded microRNAs (PLoS Path) microRNA targetomes of polyomavirus (J Virol) Innate and RNAi reciprocal inhibition (Cell) ISG15 pathway (Trends Micro) Role of viral NS1 protein (Virology) Cap-snatching (Cell) Weekly Science Picks Rich - ...
Oct 21, 2013•1 hr 14 min
Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , and Kathy Spindler Vincent, Dickson, Alan, and Kathy review clearance of simian immunodeficiency virus infection from macaques by immunization with SIV proteins encoded in a rhesus cytomegalovirus vector. Links for this episode: Immune clearance of SIV infection (Nature) Rhesus CMV vectors (Nature Medicine) Types of memory T cells (WikiPedia) Goldilocks approach to vaccines (Scilogs) Chimeric HA influenza vaccine (J Virol) Influenza va...
Oct 13, 2013•1 hr 35 min
Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Rich Condit , and Kathy Spindler In this second consecutive all-email episode, the complete TWiV team reads questions and comments from listeners about systemic antiviral responses, wild type poliovirus in Israel, Turkish scientists, viral symbiotes, and much more. Links for this episode: One Nation in support of biomedical research? Bilim Kazani (The Cauldron of Science) Science for Gezi State of Turkish Science Pathogenesis of feline...
Oct 06, 2013•2 hr 20 min
Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Rich Condit , and Kathy Spindler The complete TWiV team reads email from listeners about anti-vaccine activists, a career in microbiology, placentas, a virology textbook, the HeLa cell genome, norovirus, and much more. Links for this episode: Anthropic principle 1999 vaccine article by Alan Dove (Nature) Image credit: Jason Roberts Letters read on TWiV 251 Weekly Science Picks Dickson - A Sand County Almanac by Aldo Leopold Kathy - Noc...
Sep 29, 2013•1 hr 41 min
Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Rich Condit , and Kathy Spindler The complete TWiV crew reviews work on MERS-coronavirus, including serological studies in camels, production of an infectious DNA clone, and identification of an interferon antagonist. Links for this episode: Stephan Chron dies (NY Times) Philip Marcus dies (NY Times) Philip Marcus on TWiV #197 Ellen Fanning dies (news@Vanderbilt) Donald Low dies (CBC News) MERS timeline MERS-CoV seroepidemiology (Euros...
Sep 20, 2013•1 hr 38 min
Hosts: Vincent Racaniello and Robert Garcea Vincent and Robert recorded this episode at the 53rd ICAAC in Denver, where they talked about polyomaviruses. Links for this episode: A cornucopia of human polyomaviruses (Nat Rev Micro) Polyoma assembly factories in nucleus (PLoS Path) Overprinting gene in Merkel cell polyomavirus (PNAS) Human JCV as population marker (PLoS One) Letters read on TWiV 250 Video of this episode ( YouTube ) Weekly Science Picks Robert - The Panic Virus by Seth Mnookin Vin...
Sep 15, 2013•1 hr 32 min
Hosts: Vincent Racaniello , Dickson Despommier, Alan Dove , and Rich Condit Vincent, Dickson, Alan and Rich discuss an estimate of the number of different mammalian viruses on Earth. Links for this episode: Estimate of unknown viral diversity in mammals (mBio) How many viruses on Earth? (virology blog) Letters read on TWiV 249 Weekly Science Picks Alan - Extreme macrophotography Dickson - Baloney detection kit Rich - Mutant silkworms spin colored silk ( original article ) Vincent - FlightRadar24...
Sep 09, 2013•1 hr 31 min
Hosts: Vincent Racaniello , Dickson Despommier, and Rich Condit Vincent, Dickson, and Rich reveal how experiments with a malaria parasite lead to the introduction of a mammalian retrovirus into birds. Links for this episode: Scientists cast doubt on MERS origin (NY Times) Animal reservoirs of SARS coronavirus (Virus Res) Jo Handelsman in the White House Origins of REV (PLoS Biology) Inadvertent transfer (virology blog) Mongoose, pheasant, pox, retrovirus (PLoS Biol) Evolutionary history of REVs ...
Sep 01, 2013•1 hr 43 min
