Hosts: Vincent Racaniello , Alan Dove , Rich Condit , and Kathy Spindler Special guest: Ian Lipkin Ian joins Vincent, Alan, Rich, and Kathy to describe how his laboratory is searching for the origin of MERS-coronavirus. Links for this episode: M ERS-CoV in bats (EID) Mystery virus (NY Times) Part of MERS-CoV found in bat (virology blog() T. perforatus Frag virus (Virol J) Even Nature got it wrong! ( Nature ) Bats: Important reservoirs of emerging viruses (Clin Micro Rev) Letters read on TWiV 247...
Aug 26, 2013•1 hr 50 min
Hosts: Vincent Racaniello , Alan Dove , Rich Condit , and Kathy Spindler Vincent, Alan, Rich, and Kathy discuss the huge Pandoravirus, virologists planning H7N9 gain of function experiments, and limited access to the HeLa cell genome sequence. Links for this episode: Pandoraviruses (Science) Unlike anything seen before (virology blog) H7N9 gain of function experiments planned ( Nature , Science ) Virologists plan H7N9 gain of function experiments (virology blog) Creating a deadly virus ? Not!! (...
Aug 18, 2013•1 hr 26 min
Host: Vincent Racaniello Guests: S. Jane Flint, Lynn Enquist, Glenn Rall, and Ann Skalka The authors of the popular textbook Principles of Virology discuss how the book was conceived and written. Links for this episode: Principles of Virology Lahiere's General Virology by SE Luria Send your virology questions and comments (email or mp3 file) to twiv@twiv.tv...
Aug 11, 2013•1 hr 3 min
Hosts: Vincent Racaniello and Alan Dove Guests: Dan Barouch and Jeff Teigler This episode of TWiV was recorded before an audience at the Beth Israel Deaconess Medical Center, where Vincent and Alan spoke with Dan and Jeff about AIDS vaccines. Links for this episode: Human study of adenovirus 26 HIV-1 envelope vaccine (J Inf Dis) Seroepidemiology of adenovirus serotypes (Vaccine) SIV infection associated with expansion of gut virome (Cell, TWiV #204 ) Global approach to HIV-1 vaccine development ...
Aug 05, 2013•1 hr 23 min
Hosts: Vincent Racaniello , Rich Condit , and Kathy Spindler Guests: Rebecca Dutch and Christiane Wobus On this episode of TWiV, which was recorded before a large enthusiastic audience at the annual meeting of the American Society for Virology, Vincent, Rich, and Kathy speak with Rebecca and Christiane about their work on metapneumoviruses and noroviruses. Links for this episode: TWiV 143 with Stephanie Karst American Society for Virology Mouse model for human norovirus (mBio) Paramyxovirus fusi...
Jul 28, 2013•1 hr 10 min
Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Rich Condit , and Kathy Spindler The complete TWiV team talks about how two different viruses shape the evolution of an essential housekeeping protein. Links for this episode: Dual virus-host arms race (PLoS Biology) Dual virus-receptor duel (see comments - virology blog) Ferritin and transferrin (Protein Data Bank) Money for nothing (YouTube) Letters read on TWiV 242 Weekly Science Picks Dickson - Mantid images Kathy - No email? (phd ...
Jul 20, 2013•1 hr 33 min
Hosts: Vincent Racaniello , Alan Dove , Rich Condit , and Kathy Spindler Vincent, Alan, Rich and Kathy review how human placental trophoblasts confer viral resistance via exosome-mediated delivery of microRNAs, and isolation of the first human influenza virus in 1933. Links for this episode: H uman placental trophoblasts confer viral resistance (PNAS) Placental circulation (Wikipedia) Ovum in uterine decidua (Wikipedia) A virus obtained from influenza patients (Lancet) 80 years ago today (the gu...
Jul 14, 2013•1 hr 41 min
Host: Vincent Racaniello Guests: Markus Thali and Jason Botten Vincent travels to the University of Vermont to talk with Markus and Jason about their work on HIV, influenza virus, arenaviruses and hantaviruses. Send your virology questions and comments (email or mp3 file) to twiv@twiv.tv...
Jul 03, 2013•1 hr 14 min
Hosts: Vincent Racaniello , Alan Dove , Rich Condit, and Matt Frieman Matt joins Vincent, Alan, and Rich to summarize what we know and what we do not know about the MERS coronavirus. Links for this episode: MERS-CoV update (WHO) MERS-CoV evolution (epidemic) Sick camel linked to MERS-CoV death (7Days) MERS-CoV hospital outbreak (NEJM) MERS-CoV French hospital cluster (Lancet) MERS-CoV family cluster (NEJM) Matt on MERS-CoV on the Voice of Russia Letters read on TWiV 239 Weekly Science Picks Matt...
Jun 29, 2013•1 hr 42 min
Hosts: Vincent Racaniello , Rich Condit , and Kathy Spindler Vincent, Rich and Kathy discuss mechanisms of protein synthesis and regulation in virus-infected cells. Links for this episode: Slides for this episode (pdf) T7 ribosomal binding sites (CSH Symp Quant Biol) Tinkering with translation (CSH Persp) Translation at nexus of infection and immunity (Cell Host Microbe) Viral subversion of protein synthesis machinery (Nat Rev Micr) Weekly Science Picks Kathy - Vaccine-preventable outbreaks Rich...
Jun 23, 2013•1 hr 41 min
Hosts: Vincent Racaniello and Rich Condit Guest: Michael Emerman Vincent and Rich recorded this episode at the Fred Hutchinson Cancer Research Center in Seattle, WA, where they met up with Michael to talk about his work on the molecular and evolutionary basis of HIV replication and pathogenesis. Links for this episode: Ancient origins of SIV (PLoS Path) Paleovirology (PLoS Biology) Harmit Malik on TWiV 205 Sara Sawyer on TWiV 193 Listener Pick of the Week Molly - Facts in the case of Dr. Andrew ...
Jun 16, 2013•1 hr 21 min
Hosts: Vincent Racaniello , Alan Dove , and Kathy Spindler Vincent, Alan and Kathy review novel approaches to preventing influenza virus infection. Links for this episode: Ultraprotective influenza vaccine in mice (PNAS) Vectored immunoprophylaxis of influenza in mice (Nat Biotech) Intranasal antibody gene transfer protects agains influenza (Sci Transl Med) Estimating influenza associated deaths (CDC) Multistate HAV outbreak (CDC) Subscribe to Microbeworld video (YouTube) Save podcasting (EFF) L...
Jun 09, 2013•1 hr 29 min
Hosts: Vincent Racaniello and Rich Condit Guests: Dave Evans , Stan Lemon , and Lorne Tyrrell Vincent and Rich recorded this episode before an audience at the 2nd Li Ka Shing Institute of Virology Symposium at the University of Alberta, where they spoke with Dave, Stan, and Lorne about their work on poxvirus vaccines and recombination, an enveloped picornavirus, antivirals against hepatitis B and C viruses, and supporting virology research in Alberta. Links for this episode: Li Ka Shing Institut...
Jun 03, 2013•1 hr 17 min
Hosts: Vincent Racaniello and Kathy Spindler Guests: Nels Elde and Tom Shenk Vincent and Kathy recorded this episode before an audience at the 2013 General Meeting of the American Society for Microbiology in Denver, Colorado, where they spoke with Nels and Tom about their work on the evolution of virus-host conflict and how viruses influence the cell metabolome. Links for this episode: A cross-species view on viruses (Curr Op Virol) Pox has got a squeeze box (TWiV 198) Saturated fatty acids need...
May 26, 2013•1 hr 21 min
Hosts: Vincent Racaniello , Rich Condit , Alan Dove , and Kathy Spindler Vincent, Rich, Alan and Kathy review aerosol transmission studies of influenza H1N1 x H5N1 reassortants, H7N9 infections in China, and the MERS coronavirus. Links for this episode: Aerosol transmission of H1N1 x H5N1 reassortants (Science) It's good science (virology blog) Viral engineering dangers (Nature) Colorblind web page filter Influenza H7N9 update (ProMedMail) Influenza H7N9 risk assessment (WHO, pdf) Influenza H7N9...
May 18, 2013•1 hr 38 min
Hosts: Vincent Racaniello , Roberto Cattaneo , Reuben Harris , Lou Mansky , and Leslie Schiff Vincent meets up with Roberto, Reuben, Lou, and Leslie at the University of Minnesota to talk about their work on HIV-1, APOBEC proteins, measles virus, and teaching virology to undergraduates. Links for this episode: University of Minnesota virology timeline Revisiting HIV-1 lethal mutagenesis (Trends Micro) Exploiting HIV-1 drug repositioning (J Virol) 5-azaC induces HIV-1 lethal mutagenesis (J Virol)...
May 12, 2013•1 hr 24 min
Hosts: Vincent Racaniello , Amit Kapoor , Lan Quan , and Ian Lipkin Vincent meets up with Amit, Lan, and Ian to discuss their discovery of hepaciviruses and pegiviruses in bats and rodents. Links for this episode: Canine hepacivirus (TWiV 137) Rodent homologs of hepaciviruses and pegiviruses (mBio) Bats are a reservoir of hepaciviruses and pegiviruses (PNAS) Send your virology questions and comments (email or mp3 file) to twiv@twiv.tv...
May 05, 2013•1 hr 16 min
Hosts: Vincent Racaniello , Rich Condit , Alan Dove , and Kathy Spindler Vincent, Rich, Alan and Kathy review H7N9 infections in China, the debate over patenting genes, and receptor-binding by ferret-transmissible avian H5 influenza virus. Links for this episode: Francois Jacob dies (The Loom) WHO updates on H7N9 H7N9 is unusually dangerous (ScienceInsider) H7N9 case imported into Taiwan No H7N9 in poultry farms (Xinhua) Supreme Court considers gene patents ( TheScientist , Nature ) Patent my je...
Apr 28, 2013•1 hr 41 min
Hosts: Vincent Racaniello , Rich Condit , Dickson Despommier , and Alan Dove Vincent, Rich, Dickson, and Alan review the current status of human infections with avian influenza H7N9 virus. Links for this episode: Hilary Koprowski , 96 (NY Times) Koprowski, Kessin, Racaniello photo Listen to the Music by Roger Vaughan Influenza ocular infections ( one , two ) H7N9 global concerns (NEJM) Human infection with H7N9 (NEJM) WHO Global Alert and Response , H7N9 Avian influenza A(H7N9) virus (WHO) Novel...
Apr 21, 2013•1 hr 35 min
Hosts: Vincent Racaniello , Britt Glausinger , and Eva Harris Vincent visits the University of California at Berkeley and speaks with Britt Glaunsinger and Eva Harris about their work on Kaposi's sarcoma associated herpesvirus and dengue virus. Thanks to the Microbiology Graduate Students for hosting me at their annual symposium, and especially to Emma, Lisa, and Zoe for their wonderful hospitality during my stay. Links for this episode: RNA element in IL-6 confers SOX resistance (J Virol) Dual ...
Apr 14, 2013•1 hr 8 min
Hosts: Vincent Racaniello , Rich Condit , Dickson Despommier , Alan Dove , and Kathy Spindler The complete TWiV team reviews the controversial publication of the HeLa cell genome, a missing vial of Guanarito virus in a BSL-4 facility, and human infections with avian influenza H7N9 virus. Links for this episode: Containment plan for poliovirus (pdf, WHO) Post-eradication of polio Ocular tropism of respiratory viruses (MMBR) Rally for medical research HeLa genome sequence withdrawn (ScienceInsider...
Apr 07, 2013•1 hr 35 min
Hosts: Vincent Racaniello , Dickson Despommier , and Terry Dermody Vincent and Dickson speak with Terry Dermody about his career in medicine and virology. Send your virology questions and comments (email or mp3 file) to twiv@twiv.tv
Mar 30, 2013•1 hr 33 min
Hosts: Vincent Racaniello , Alan Dove , and Kathy Spindler Vincent, Rich, and Kathy read listener comments and questions on viral oncotherapy, science communication, a functional HIV cure in an infant, and much more. Links for this episode: Plant viruses alter insect behavior (Sci Rep) Circovirus in Shanghai Letters read on TWiV 225 Weekly Science Picks Kathy - Case stories about CoV-EMC ( one , two )Rich - The Self-Illusion by Bruce HoodVincent - Moving Targets Listener Pick of the Week Liam - ...
Mar 23, 2013•1 hr 41 min
Hosts: Vincent Racaniello , Alan Dove , and Kathy Spindler Vincent, Alan, Kathy, and Dickson discuss identification of a cell receptor for the coronavirus-EMC, and the role of interferon-epsilon in protecting the female reproductive tract. Links for this episode: Dipeptidyl peptidase 4 is CoV-EMC receptor (Nature) Receptor for new coronavirus (Nature) Broad reception for coronavirus (Nature) SARS 10th anniversary (Science) Interferon-epsilon protects female reproductive tract (Science) Families ...
Mar 17, 2013•1 hr 35 min
Hosts: Vincent Racaniello , Alan Dove , and Kathy Spindler Vincent, Alan, and Kathy discuss new influenza virus NA inhibitors, detection of EEEV antibody and RNA in snakes, and replication of the coronavirus EMC in human airway epithelial cells. Links for this episode: International Women's Day 2013 Condit Dam, 6 weeks later (Vimeo) Influenza HA assay Covalent influenza NA inhibitors (ScienceExpress) Defective influenza HA mutants (Virology) New flu drug ( BBC , e! Science ) EEEV antibody, RNA i...
Mar 10, 2013•1 hr 29 min
Hosts: Vincent Racaniello , Rich Condit , Dickson Despommier , Alan Dove , and Kathy Spindler The complete TWiV team discusses the amazing finding that cyclic GMP-AMP synthase is a cytosolic innate immune DNA sensor. Links for this episode: Zhijian J. Chen (Wikipedia) Sensing the dark side of DNA (Science) cGAMP is an endogenous second messenger (Science) cGAMP synthase is a DNA sensor (Science) Pore-forming toxin (Wikipedia) Letters read on TWiV 222 Weekly Science Picks Kathy - Graphene batteri...
Mar 03, 2013•1 hr 37 min
Hosts: Vincent Racaniello , Dickson Despommier , and Kathy Spindler Vincent, Dickson, and Kathy review two emerging bunyaviruses, SFTSV and SBV. Links for this episode: Human transmission of SFTSV (JID) SFTSV serosurvey (EID) SFTSV on TWiV 147 SFTSV in Japan (ProMedMail) SFTSV mouse model (PNAS) SBV infectious DNA and mouse model (PLoS Path) SBV in Sweden (ProMedMail) Letters read on TWiV 221 Weekly Science Picks Kathy - Winners, 2012 Science & Engineering Visualization Challenge Vincent - T...
Feb 24, 2013•1 hr 31 min
Hosts: Vincent Racaniello , Rich Condit, Alan Dove , and Kathy Spindler Vincent, Rich, Alan, and Kathy discuss regulation of influenza virus replication by splicing, and the bacteriophage T7 random walk. Links for this episode: Influenza splicing regulates infection (Cell Reports) Reporting on flu splicing from NPR , BBC , NBC Splicing of influenza RNA 8 (jpg) Bacteriophage T7 remodeling during infection (Science) Cryo-ET of intact cells (Trends Cell Biol) T7: The Movie (YouTube) Cry0-EM vs Cryo...
Feb 17, 2013•1 hr 35 min
Hosts: Vincent Racaniello , Rich Condit , and Anthony S. Fauci Vincent and Rich meet up with Anthony S. Fauci, MD, Director of the National Institute of Allergy and Infectious Diseases. Links for this episode: Fauci history (J Clin Inves) Fauci Pharmacy Anthony Fauci (Wikipedia) Anthony Fauci: Unfinished business (Lens) Send your virology questions and comments (email or mp3 file) to twiv@twiv.tv...
Feb 10, 2013•1 hr 4 min
Hosts: Vincent Racaniello , Alan Dove , and Welkin Johnson Vincent, Alan, and Welkin discuss how endogenous retroviruses in mice are held in check by the immune response. Links for this episode: Kuan-Teh Jeang , 54 Resurrection of ERVs (Nature) Toll-like receptors control ERVs (Immunity) Retroviral danger from within (Immunity) Ancient viruses wreak havoc (The Scientist) Letters read on TWiV 218 Weekly Science Picks Welkin - Viruses: Essential Agents of Life ( review at STC )Alan - Infinity Imag...
Feb 03, 2013•1 hr 29 min
