Hosts: Vincent Racaniello , Alan Dove , and Kathy Spindler Vincent, Alan, Kathy, and Dickson discuss identification of a cell receptor for the coronavirus-EMC, and the role of interferon-epsilon in protecting the female reproductive tract. Links for this episode: Dipeptidyl peptidase 4 is CoV-EMC receptor (Nature) Receptor for new coronavirus (Nature) Broad reception for coronavirus (Nature) SARS 10th anniversary (Science) Interferon-epsilon protects female reproductive tract (Science) Families ...
Mar 17, 2013•1 hr 35 min
Hosts: Vincent Racaniello , Alan Dove , and Kathy Spindler Vincent, Alan, and Kathy discuss new influenza virus NA inhibitors, detection of EEEV antibody and RNA in snakes, and replication of the coronavirus EMC in human airway epithelial cells. Links for this episode: International Women's Day 2013 Condit Dam, 6 weeks later (Vimeo) Influenza HA assay Covalent influenza NA inhibitors (ScienceExpress) Defective influenza HA mutants (Virology) New flu drug ( BBC , e! Science ) EEEV antibody, RNA i...
Mar 10, 2013•1 hr 29 min
Hosts: Vincent Racaniello , Rich Condit , Dickson Despommier , Alan Dove , and Kathy Spindler The complete TWiV team discusses the amazing finding that cyclic GMP-AMP synthase is a cytosolic innate immune DNA sensor. Links for this episode: Zhijian J. Chen (Wikipedia) Sensing the dark side of DNA (Science) cGAMP is an endogenous second messenger (Science) cGAMP synthase is a DNA sensor (Science) Pore-forming toxin (Wikipedia) Letters read on TWiV 222 Weekly Science Picks Kathy - Graphene batteri...
Mar 03, 2013•1 hr 37 min
Hosts: Vincent Racaniello , Dickson Despommier , and Kathy Spindler Vincent, Dickson, and Kathy review two emerging bunyaviruses, SFTSV and SBV. Links for this episode: Human transmission of SFTSV (JID) SFTSV serosurvey (EID) SFTSV on TWiV 147 SFTSV in Japan (ProMedMail) SFTSV mouse model (PNAS) SBV infectious DNA and mouse model (PLoS Path) SBV in Sweden (ProMedMail) Letters read on TWiV 221 Weekly Science Picks Kathy - Winners, 2012 Science & Engineering Visualization Challenge Vincent - T...
Feb 24, 2013•1 hr 31 min
Hosts: Vincent Racaniello , Rich Condit, Alan Dove , and Kathy Spindler Vincent, Rich, Alan, and Kathy discuss regulation of influenza virus replication by splicing, and the bacteriophage T7 random walk. Links for this episode: Influenza splicing regulates infection (Cell Reports) Reporting on flu splicing from NPR , BBC , NBC Splicing of influenza RNA 8 (jpg) Bacteriophage T7 remodeling during infection (Science) Cryo-ET of intact cells (Trends Cell Biol) T7: The Movie (YouTube) Cry0-EM vs Cryo...
Feb 17, 2013•1 hr 35 min
Hosts: Vincent Racaniello , Rich Condit , and Anthony S. Fauci Vincent and Rich meet up with Anthony S. Fauci, MD, Director of the National Institute of Allergy and Infectious Diseases. Links for this episode: Fauci history (J Clin Inves) Fauci Pharmacy Anthony Fauci (Wikipedia) Anthony Fauci: Unfinished business (Lens) Send your virology questions and comments (email or mp3 file) to twiv@twiv.tv...
Feb 10, 2013•1 hr 4 min
Hosts: Vincent Racaniello , Alan Dove , and Welkin Johnson Vincent, Alan, and Welkin discuss how endogenous retroviruses in mice are held in check by the immune response. Links for this episode: Kuan-Teh Jeang , 54 Resurrection of ERVs (Nature) Toll-like receptors control ERVs (Immunity) Retroviral danger from within (Immunity) Ancient viruses wreak havoc (The Scientist) Letters read on TWiV 218 Weekly Science Picks Welkin - Viruses: Essential Agents of Life ( review at STC )Alan - Infinity Imag...
Feb 03, 2013•1 hr 29 min
Hosts: Vincent Racaniello , Alan Dove , Rich Condit , and Dickson Despommier Vincent, Alan, Rich, and Dickson review influenza vaccines. Links for this episode: FDA licensed flu vaccines Seasonal influenza vaccine distributed How flu vaccine strains are selected ( one , two ) WHO report on flu vaccine strain selection (pdf) Derek Smith on antigenic mapping Salk's 1945 influenza vaccine TIV preparation: Fluzone , Agriflu , Fluvirin , Fluarix , Flulaval , Afluria (pdfs) LAIV preparation (pdf) Effi...
Jan 26, 2013•1 hr 35 min
Hosts: Vincent Racaniello , Alan Dove , Rich Condit , and Kathy Spindler Vincent, Alan, Rich, and Kathy resume the virology 101 series with a discussion of RNA capping, splicing, and export. Links for this episode: Slides for this episode (pdf) Spliced Ad 2 late mRNAs (Cell) An amazing sequence arrangement (Cell) Adenovirus late mRNA undecanucleotide (Cell) Aaron Shatkin, 72 (virology blog) Schrödinger's cat (Wikipedia) Letters read on TWiV 216 Weekly Science Picks Rich - Linus Pauling's explana...
Jan 20, 2013•1 hr 33 min
Hosts: Vincent Racaniello , Alan Dove , and Kathy Spindler Vincent, Alan, and Kathy review the finding that rabies virus infection alters but does not kill neurons, and provide an update on the novel coronavirus in the Middle East. Links for this episode: Neuronal survival after rabies virus infection (PLoS Path) Clusters of coronavirus cases (Nature) ProMedMail on novel coronavirus Novel coronavirus outbreak news (Int J Inf Dis) Genomic characterization of novel coronavirus (mBio) Broad cell tr...
Jan 13, 2013•1 hr 32 min
Hosts: Vincent Racaniello , Rich Alan Dove , and Kathy Spindler Vincent, Alan, and Kathy discuss how coagulation factor X binding to adenovirus activates the innate immune system, and a novel polyomavirus associated with brain tumors in raccoons. Links for this episode: Coagulation factor X activates innate immunity to adenovirus (Science) A decorated virus cannot hide (Science) Reflecting on goals for Science Polyomavirus associated with raccoon brain tumors (EID) MPNST Moore tumor viruses - TW...
Jan 07, 2013•1 hr 18 min
Hosts: Vincent Racaniello , Rich Condit , Alan Dove , and Kathy Spindler In their final episode of the year, the TWiV team reviews twelve cool virology stories from 2012. Twelve virology stories from 2012: H5N1 (TWiV 190 , 182 , 177 , 173 , 168 ) Polymorphisms that control susceptibility (TWiV 175 , 180 ) New virus receptors (TWiV 166 , 210 ) XMRV: Last nail in the coffin ( TWiV special ) Polio eradication troubles in Pakistan (NY Times one , two , three ; virology blog ) Biomedical research cri...
Dec 30, 2012•2 hr 2 min
Hosts: Vincent Racaniello and Stuart Firestein Vincent and Stuart discuss why ignorance - all of what we don't know, and even what we don't know we don't know - is the driving force of science. Links for this episode: Ignorance: How it drives Science by Stuart Firestein Ignorance website Send your virology questions and comments (email or mp3 file) to twiv@twiv.tv...
Dec 29, 2012•1 hr 21 min
Hosts: Vincent Racaniello , Rich Condit , Alan Dove , and Kathy Spindler The TWiVerers answer listener email about genetically modified chickens, a hendra vaccine for horses, online education, curing color blindness, Roosevelt and polio, Th cells, and much more. Links for this episode: TWiV 116: Cocaine, colonies, and chickens Glofish Recombinant Hendra vaccine Gene therapy for color blindness (Nature) FDR, polio, and Guillain-Barré Transcript of TWiV 197: Philip Marcus (pdf) TWiV 10: Bats, elep...
Dec 24, 2012•1 hr 48 min
Hosts: Vincent Racaniello , Rich Condit , Alan Dove , and Kathy Spindler The TWiV four discuss an mRNA-based influenza vaccine, and a phage tubulin that forms a filamentous array in the host cell that is needed for positioning viral DNA. Links for this episode: mRNA vaccine against influenza (Nat Biotech) Phage tubulin assembles dynamic filaments (Cell) Viral skeleton (The Scientist) Not unorganized bags of enzymes (TWiM 28) Bacterial cytoskeleton (FEMS Micro Rev) Triangulation number (jpg) Lett...
Dec 16, 2012•1 hr 37 min
Hosts: Vincent Racaniello , Rich Condit , Dickson Despommier , Alan Dove , and Kathy Spindler The complete TWiV team reviews identification of the cell receptor for hepatitis B and D viruses, and the cell enzyme that cleaves the genome-linked protein from picornaviral RNA. Links for this episode: HBV and HDV cell receptor (eLIFE) Getting to grips with hepatitis (eLIFE) Sodium taurocholate Poliovirus hijacks DNA repair enzyme (PNAS) Letters read on TWiV 210 Weekly Science Picks Rich - Vendée Glob...
Dec 09, 2012•1 hr 33 min
Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , and Kathy Spindler Vincent, Dickson, Alan, and Kathy answer listener email about deformed wing virus, West Nile virus, FluMist, influenza in Canada, viruses and the tree of life, and more. Links for this episode: Postcard from the ultimate student athlete (pdf) Fidelity of protein synthesis (Biochemistry, 5th ed.) Letters read on TWiV 209 Weekly Science Picks Dickson - Cassini web site and Mars reconnaissance orbiter Alan - OneZoom Tre...
Dec 02, 2012•1 hr 53 min
Hosts: Vincent Racaniello and Jon Yewdell Vincent and is joined by special guest Jon Yewdell to discuss solutions for ending the current crisis in American biomedical research. Links for this episode: How to succeed in science: Taking the plunge (Nat Rev Mol Cell Biol) How to succeed in science: Making discoveries (Nat Rev Mol Cell Biol) How to succeed in science (YouTube) Resources for graduate students and postdocs Science career cartoons by Alexander Dent Send your virology questions and comm...
Nov 25, 2012•1 hr 27 min
Hosts: Vincent Racaniello , Alan Dove , Matt Frieman , and Kathy Spindler Vincent, Alan, Matt, and Kathy review the use of silk to stabilize antibiotics and a viral vaccine, and an impaired-fidelity vaccine against SARS coronavirus. Links for this episode: Silk stabilization of antibiotics and vaccines (PNAS) Impaired-fidelity coronavirus vaccine (Nature Medicine) Select agent status and SARS therapies (Spoonful of Medicine) Letters read on TWiV 207 Weekly Science Picks Matt - Virus-associated p...
Nov 19, 2012•1 hr 46 min
Hosts: Vincent Racaniello , Alan Dove , Dickson Despommier , and Kathy Spindler Vincent, Alan, Dickson, and Kathy discuss how the innate immune response to viral infection influences the production of pluripotent stem cells, and the diverse mobilome of giant viruses. Links for this episode: Innate immunity and nuclear reprogramming (Cell) Viruses affect cell reprogramming (The Scientist) Cell-penetrating peptides (Trends Mol Med) Provirophages and transpovirons (PNAS) Organic Lake photo and map ...
Nov 11, 2012•1 hr 40 min
Hosts: Vincent Racaniello and Harmit Malik Vincent is joined by special guest Harmit Malik to discuss his work on the evolution of genetic conflict. Links for this episode: Harmit on YouTube Retroviruses and mammalian placentation (PNAS) Paleovirology (Curr Opin Virol) Evolution among AIM2-like receptors (J Exp Med) Poxvirus genomic accordions (Cell) Letters read on TWiV 205 Weekly Science Picks Vincent - Candidates' answers to science questions Listener Pick of the Week Steve - Mural of Henriet...
Oct 25, 2012•1 hr 43 min
Hosts: Vincent Racaniello , Alan Dove , Matt Frieman , and Kathy Spindler Vincent, Alan, Matt and Kathy review isolation of a new coronavirus from two patients in the Middle East, and expansion of the enteric virome during simian AIDS. Subscribe to TWiV (free) in iTunes , at the Zune Marketplace , by the RSS feed , by email , or listen on your mobile device with the Microbeworld app . Links for this episode: Novel coronavirus (virology blog one , two ) PCR assay for novel CoV (Eurosurveillance) ...
Oct 21, 2012•1 hr 36 min
Hosts: Vincent Racaniello , Rich Condit , and Mark Challberg Vincent and Rich meet up with Mark Challberg to talk about his scientific career studying viral DNA replication, and his transition to an NIH Program Officer. Subscribe to TWiV (free) in iTunes , at the Zune Marketplace , by the RSS feed , by email , or listen on your mobile device with the Microbeworld app . Links for this episode: Purification of vaccinia virus DNA polymerase (J Biol Chem) Adenovirus DNA replication in vitro (PNAS) H...
Oct 14, 2012•1 hr 36 min
Hosts: Vincent Racaniello , T. Jack Morris , James Van Etten , and Charles Wood Vincent travels to the University of Nebraska to meet with members of the Nebraska Center for Virology and discuss their work on algal viruses, plant viruses, HIV and Kaposi's sarcoma herpesvirus. Links for this episode: The Nebraska Center for Viology Behind the scenes at TWiV 202 TWiV on Facebook Weekly Science Picks Charles - Rhabdovirus associated with hemorrhagic fever (PLoS Path)Vincent - Bacteriophage graffiti...
Oct 07, 2012•1 hr 12 min
Hosts: Vincent Racaniello , Alan Dove , Rich Condit , and Kathy Spindler Vincent, Alan, Rich, and Kathy answer reader email about rabies, xenotransplantation, poliovirus, Ph.D. programs, mosquitoes, and much more. Links for this episode: Mattias Schnell Novel rhabdovirus from Africa (Plos Path) Milwaukee protocol (Wikipedia) How lethal is rabies virus? (virology blog) Issues with polio eradication (J Gen Virol) Vaccine-derived polioviruses (J Inf Dis) Global pandemics with Hidde Ploegh (Vimeo) P...
Sep 30, 2012•2 hr 5 min
Hosts: Vincent Racaniello , Alan Dove , Rich Condit , Elke Mühlberger , Paul Duprex , and Ron Corley Vincent, Alan, and Rich celebrate the 200th episode of TWiV by visiting the National Emerging Infectious Diseases Laboratories at Boston University Medical Center, where they meet with Elke, Paul, and Ron to talk about building and working in a BSL4 facility. Links for this episode: The NEIDL NEIDL goes public (BU Today) Biosafety levels (Wikipedia) Select agents (Wikipedia) NEIDL stalled (Boston...
Sep 23, 2012•1 hr 40 min
Hosts: Vincent Racaniello and W. Ian Lipkin Vincent and Ian review a multicenter blinded analysis which finds no association between chronic fatigue syndrome/myalgic encephalomyelitis and XMRV or polytropic murine leukemia virus. Subscribe to TWiV (free) in iTunes , at the Zune Marketplace , by the RSS feed , by email , or listen on your mobile device with the Microbeworld app . Links for this episode: Multicenter blinded analysis of CFS and XMRV/pMLV (mBio) Press release on multicenter study (p...
Sep 18, 2012•44 min
Hosts: Vincent Racaniello , Alan Dove , Rich Condit , and Kathy Spindler Vincent, Alan, Rich, and Kathy discuss recent outbreaks of hantavirus pulmonary syndrome in Yosemite National Park and novel swine-origin influenza in the US midwest, and isolation of the Heartland virus from two patients in Missouri with severe febrile illness. Subscribe to TWiV (free) in iTunes , at the Zune Marketplace , by the RSS feed , by email , or listen on your mobile device with the Microbeworld app . Links for th...
Sep 16, 2012•1 hr 41 min
Hosts: Vincent Racaniello , Alan Dove , Rich Condit , and Kathy Spindler Vincent, Alan, Rich, and Kathy review fatal avian influenza virus in harbor seals, and poxvirus deployment of genomic accordions to counter antiviral defenses. Links for this episode: Avian influenza virus in harbor seals (mBio) Poxvirus genomic accordions (mBio) Red Queen hypothesis (Wikipedia) TWiV on Facebook Letters read on TWiV 198 Weekly Science Picks Alan - Getting Better: 200 Years of Medicine Rich - Khan Academy Ka...
Sep 02, 2012•1 hr 39 min
Hosts: Vincent Racaniello and Philip I. Marcus Vincent travels to the University of Connecticut to meet up with Professor Philip I. Marcus to discuss his development of the single cell cloning technique in the early 1950s. Links for this episode: Cloning with X-irradiated feeder cells (PNAS) Philip I. Marcus in the lab (jpg) Cloning with capillary tubes (jpg) Cloning platform (jpg) VRR holding 1950s glass plate of clones (jpg) HeLa clones (jpg) Cloning cylinder (jpg) TWiV on Facebook Coming soon...
Aug 26, 2012•1 hr 11 min
