Sean Whelan returns to TWiV to speak with Vincent about using haploid cell lines to identify genes encoding cell receptors for viruses, including Ebolavirus, Lassa virus, and more. Host: Vincent Racaniello Guest: Sean Whelan Become a patron of TWiV! Links for this episode Ebola virus entry requires the cholesterol transporter Niemann-Pick C1 (Nature) Lassa virus entry requires a trigger-induced receptor switch (Science) Infectious Entry Pathway Mediated by the Human ERV K Envelope Protein (J Vir...
Mar 25, 2018•1 hr 3 min•Ep. 486
The TWiV posse considers viral insulin-like peptides encoded in fish genomes, and insect antiviral immunity by production of viral DNA from defective genomes of RNA viruses. Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Kathy Spindler , and Brianne Barker Become a patron of TWiV! Links for this episode ASM Microbe 2018 Insulin-like peptides in Iridovirus genomes (PNAS) Dicer-2 dependent generation of cvDNA from defective genomes (Cell Host Micr) Carla Saleh on insect antiviral imm...
Mar 18, 2018•1 hr 26 min•Ep. 485
The TWiVumvirate discuss the giant Tupanvirus, with the longest tail in the known virosphere, and dampened STING dependent interferon activation in bats. Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Kathy Spindler , and Brianne Barker Become a patron of TWiV! Links for this episode ASM Microbe 2018 Tailed giant Tupanvirus (Nat Commun) Soda Lakes (Wikipedia) Dampened STING in bats (Cell Host Microbe) Image credit Letters read on TWiV 484 Weekly Science Picks Kathy - How to read a ...
Mar 11, 2018•1 hr 27 min•Ep. 484
Vincent and the Virals review undermining of antiviral effectiveness by genital inflammation, and heterogeneity of influenza virus infection in single cells. Hosts: Vincent Racaniello , Alan Dove , and Kathy Spindler Become a patron of TWiV! Links for this episode USA Science and Engineering Festival Genital inflammation and antiviral effectiveness (Nat Med) Single cell infection heterogeneity (eLife) Single-cell poliovirus infection (Virology) Image credit Letters read on TWiV 483 Weekly Scienc...
Mar 04, 2018•1 hr 41 min•Ep. 483
The TWiV Masters discuss serologic evidence of Ebolavirus infection in a population with no outbreaks, and the set of endogenous viral elements in the mosquito genome. Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Kathy Spindler , and Brianne Barker Become a patron of TWiV! Links for this episode USA Science and Engineering Festival Ebolavirus infection without an outbreak (J Inf Dis) A. aegypti EVEome (Curr Biol) One Loop to Rule Them All (Trends Biochem Sci) Image credit Letters...
Feb 25, 2018•1 hr 38 min•Ep. 482
The TWiVodrome considers the intestinal tract as an alternative infection route for MERS coronavirus, and how reduced accumulation of defective viral RNAs might lead to severe influenza. Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Rich Condit , and Kathy Spindler Become a patron of TWiV! Links for this episode HaHa influenza virus (mBio) MERS coronavirus intestinal transmission (Sci Adv) Simulated gastric and intestinal fluids Stomacher Defective genomes and severe influenza (PL...
Feb 18, 2018•1 hr 42 min•Ep. 481
Scott Hensley joins the TWiVites to review the current influenza season and presence of the virus in exhaled breath of symptomatic cases. Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Rich Condit , and Kathy Spindler Guest: Scott Hensley Become a patron of TWiV! Links for this episode Weekly influenza surveillance (CDC) Influenza vaccination status (CDC) H3N2 glycosylation site and eggs (PNAS) Influenza virus in exhaled breath (PNAS) Geometric Defects and Icosahedral Viruses (Viru...
Feb 11, 2018•1 hr 54 min•Ep. 480
The TWiVome explores induction of antiviral responses by repeating patterns of capsids, and a fungus in the mosquito gut that aids dengue virus replication. Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Rich Condit , and Kathy Spindler Become a patron of TWiV! Links for this episode Innate immune detection of capsid patterns (mBio) Mosquito gut fungus aids dengue virus replication (eLife) Nominate MicrobeTV for a Keck award Image credit Letters read on TWiV 479 Weekly Science Pick...
Feb 04, 2018•1 hr 51 min•Ep. 479
The TWiV team explains how infectious horsepox virus - likely the ancestor of smallpox vaccines - was recovered from chemically synthesized DNA fragments. Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Rich Condit , and Kathy Spindler Become a patron of TWiV! Links for this episode ASV 2018: asv.org , asv2018.umd.edu Potential Zika virus related birth defects , USA (MMWR) Bordering on an outbreak (Tex Obs) Horsepox virus from synthesized DNA (PLoS One) Horsepox based smallpox vacci...
Jan 28, 2018•1 hr 41 min•Ep. 478
The TWiVodrome explains how a gag-like protein from a retrotransposon forms virus-like particles that carry mRNA within vesicles across the synapse. Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Rich Condit , and Kathy Spindler Become a patron of TWiV! Links for this episode ASV 2018: asv.org , asv2018.umd.edu Arc virus-like particles transport RNA across the synapse ( mammalian , Drosophila ) Chemical synapse (Wikipedia) 2 minute video on the neuron Image credit Letters read on T...
Jan 20, 2018•1 hr 43 min•Ep. 477
Nels joins the TWiV team to talk about his work on genomic accordions in vaccinia virus, hepatitis B virus in a 439 year old mummy, and viral induction of energy synthesis by a long noncoding RNA. Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Rich Condit , and Kathy Spindler Guest: Nels Elde Click arrow to play Download TWiV 476 (63 MB .mp3, 105 min) Subscribe (free): iTunes , RSS , email Become a patron of TWiV! Links for this episode ASV 2018: asv.org , asv2018.umd.edu TWiV is a...
Jan 14, 2018•1 hr 45 min•Ep. 476
In the first episode for 2018, the TWiV team reviews the amazing virology stories of 2017. Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , and Kathy Spindler Become a patron of TWiV! Links for this episode ASV 2018: asv.org , asv2018.umd.edu Virology stories of 2017 - in no particular order A virologist in space - TWiV 444 Cool things viruses do: Astrovirus capsids are enterotoxins TWiV 423; giant Klosneuviruses encode lots of stuff for translation TWiV 437 ; Giant Noumeaviruses qui...
Jan 07, 2018•1 hr 50 min•Ep. 475
The TWiVanguardians take on Bodo saltans virus, a leviathan which infects an abundant flagellated eukaryote in Earth's waters. Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , and Kathy Spindler Become a patron of TWiV! Links for this episode ASV 2018: asv.org , asv2018.umd.edu Support Microbe.tv Anti-dengue antibody and disease (J Inf Dis) Bodo saltans virus (biorXiv) Kinetoplast DNA (Euk Cell) Crossword puzzle for book contest (pdf) Letters read on TWiV 474 Weekly Science Picks Kat...
Dec 31, 2017•1 hr 43 min•Ep. 474
The Fellowship of the Virus discuss enhancement of dengue disease in humans: the contribution of antibody concentration and increased binding to Fc receptors. Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Rich Condit , and Kathy Spindler Become a patron of TWiV! Links for this episode ASV 2018: asv.org , asv2018.umd.edu Support Microbe.tv NEIDL is approved NIH lifts GOF moratorium (Science) Blackburn steps down as head of Salk Institute (SD Union Trib) ADE of dengue disease in hum...
Dec 24, 2017•1 hr 50 min•Ep. 473
Vincent speaks with professional podcast producer Ray Ortega about his career and the power of the podcast. Host: Vincent Racaniello Guest: Ray Ortega Become a patron of TWiV! Links for this episode Ray on Twitter The Podcasters' Studio Produce Picker Podcast Ray's YouTube channel Intro music is by Ronald Jenkees . Send your virology questions and comments to twiv@microbe.tv...
Dec 20, 2017•1 hr
The TWiV team reveal the origin of the poxvirus membrane, and how a retrovirus drove the development of the placenta of a lizard. Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Rich Condit , and Kathy Spindler Become a patron of TWiV! Links for this episode ASV 2018: asv.org , asv2018.umd.edu Support Microbe.tv Origin of poxvirus membrane (PNAS) Poxvirus movies: Movie S1 , Movie S2 , Movie S3 , Movie S4 , Movie S5 Lizard placenta from a retrovirus (PNAS) Letters read on TWiV 472 We...
Dec 17, 2017•1 hr 54 min•Ep. 472
The TWiVerinos discuss restriction of dengue virus vaccine by Sanofi, and data which suggest that Dengvaxia causes enhanced disease in previously uninfected recipients. Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Rich Condit , and Kathy Spindler Become a patron of TWiV! Links for this episode ASV 2018: asv.org , asv2018.umd.edu Sanofi update on D engvaxia Trouble for dengue vaccine (Reuters) Sanofi restricts dengue vaccine (CIDRAP) Dengvaxia enhances disease (Vaccine) Interview ...
Dec 10, 2017•1 hr 48 min•Ep. 471
The TWiV ninjas reveal that bacteriophage particles rapidly move across monolayers of eukaryotic cells from different tissues. Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Rich Condit , and Kathy Spindler Become a patron of TWiV! Links for this episode Transcytosis of bacteriophage across cell layers (mBio) Image credit Letters read on TWiV 470 This episode is brought to you by Blue Apron. Blue Apron is the #1 fresh ingredient and recipe delivery service in the country. Get $30 o...
Dec 03, 2017•1 hr 57 min•Ep. 470
The TWiV hosts discuss a plant virus that infects a fungus, and whether you need to work insane hours to succeed in science. Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Rich Condit , and Kathy Spindler Become a patron of TWiV! Links for this episode Phytopathogenic fungus hosts a plant virus (PNAS) Franklin column (PNAS) Potato famine mystery solved (History) Protein Data Bank You do not need to work 80 hours per week (THE) How I Learned To Stop Worrying (Sci Am) Image credit: T...
Nov 26, 2017•1 hr 48 min•Ep. 469
Amy joins the TWiV team to talk about her career and her work on Zika virus neurotropism using embryonic mouse organotypic brain slice cultures. Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Rich Condit , and Kathy Spindler Guest: Amy Rosenfeld Become a patron of TWiV! Links for this episode Grad students face tax hike (Nature) House voted to bankrupt grad students (NYTimes) Neurotropism of early and late Zika virus isolates (PNAS) Zika virus blocks the neuron road (virology blog)...
Nov 19, 2017•1 hr 47 min•Ep. 468
Jon and Teddy Yewdell join the TWiV team to talk about their careers, their research, and the problems with biomedical research. Hosts: Vincent Racaniello , Alan Dove ,and Rich Condit Guest: Jon Yewdell and Teddy Yewdell Become a patron of TWiV! Links for this episode Jon Yewdell laboratory Chaudhuri laboratory Truth Wins by Jon Yewdell ( epub or mobi download) Noncoding RNAs and class switch recombination (Int Imm) Translating DRiPs (J Leuk Biol) Lamprey VLRP response to influenza virus (eLife)...
Nov 12, 2017•1 hr 40 min•Ep. 467
From Indiana University, Vincent and Kathy speak with Tuli Mukhopadhyay, John Patton, and Adam Zlotnick about their careers and their work on alphaviruses, hepatitis B virus, and rotaviruses. Host: Vincent Racaniello and Kathy Spindler Guests: Tuli Mukhopadhyay , John Patton , and Adam Zlotnick Become a patron of TWiV! Links for this episode Mukhopadhyay lab Patton lab Zlotnick lab Tuli on Twitter Palmitoylation of Sindbis virus TF protein (J Virol) Hepatitis B virus structure (Ann Rev Virol) Ro...
Nov 05, 2017•1 hr 6 min•Ep. 466
Theodora Hatziioannou joins the TWiV team to discuss a macaque model for AIDS, and how a cell protein that blocks HIV-1 infection interacts with double-stranded RNA. Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Rich Condit , and Kathy Spindler Guest: Theodora Hatziioannou Become a patron of TWiV! Links for this episode ASU-UofA Joint Virology Symposium Laboratory of Retrovirology , Rockefeller University HIV-1-induced AIDS in monkeys (Science) APOBEC3H bound to duplex RNA (Nat Co...
Oct 29, 2017•1 hr 50 min•Ep. 465
At Tufts University Dental School in Boston, Vincent speaks with Katya Heldwein and Sean Whelan about their careers and their work on herpesvirus structure and replication of vesicular stomatitis virus. Host: Vincent Racaniello Guests: Katya Heldwein and Sean Whelan Become a patron of TWiV! Links for this episode Crystal structure of HSV gB (Science) Crystal structure of HSV fusion regulator gH-gL (Nat Struct Mol Biol) Nuclear Exodus: Herpesviruses Lead the Way (Ann Rev Virol) VSV Pseudotypes Be...
Oct 22, 2017•1 hr 28 min•Ep. 464
The TWiViridae review the 2017 Nobel Prizes for cryoEM and circadian rhythms, and discuss modulation of plant virus replication by RNA methylation. Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Rich Condit , and Kathy Spindler Become a patron of TWiV! Links for this episode Truth Wins by Jonathan Yewdell ( epub or mobi ) Gabriel Victora awarded MacArthur Prize Forty Years of mRNA Splicing (CSH) 2017 Chemistry Nobel: Jacques Dubochet , Joachim Frank , Richard Henderson 2017 Physiol...
Oct 15, 2017•1 hr 44 min•Ep. 463
David Tuller returns to discuss his efforts to expose the methodological and ethical problems with the PACE trial for ME/CFS. Hosts: Vincent Racaniello Guest: David Tuller Become a patron of TWiV! Links for this episode All of David's ME/CFS articles at virology blog David Tuller on Twitter Through the Shadowlands by Julie Rehmeyer Intro music is by Ronald Jenkees . Send your virology questions and comments to twiv@microbe.tv...
Oct 14, 2017•1 hr 8 min
Vincent speaks with 1993 Nobel Laureate Phillip A. Sharp about his career and his seminal discovery of RNA splicing in mammalian cells, which changed our understanding of gene structure. Hosts: Vincent Racaniello Guest: Phillip A. Sharp Become a patron of TWiV! Links for this episode Meet the Microbiologist Sharp Laboratory Spliced segments of adenovirus late mRNA (PNAS) Video of this interview (YouTube) This episode is brought to you by the Defense Threat Reduction Agency. Part of the U.S. Depa...
Oct 07, 2017•39 min•Ep. 462
The TWiVers discuss the declining readability of scientific texts, and review the use of self-inactivating rabies virus for tracing neural circuits. Hosts: Vincent Racaniello , Dickson Despommier , Rich Condit , and Kathy Spindler Guest: Brianne Barker Become a patron of TWiV! Links for this episode Meet the Microbiologist TWiV 1 : West Nile Virus Decreasing readability of scientific texts (eLife) Measure text readability Tracing neural circuits with self-inactivating rabies virus (Cell) Cre dri...
Sep 30, 2017•2 hr 7 min•Ep. 461
Vincent travels to the University of Pennsylvania and speaks with virologists Gary Cohen, Scott Hensley, Carolina Lopez, and Susan Weiss about their careers and their research. Host: Vincent Racaniello Guests: Gary Cohen , Scott Hensley , Carolina Lopez , and Susan Weiss Become a patron of TWiV! Links for this episode Cohen Laboratory Hensley Laboratory Lopez Laboratory Weiss Laboratory This episode is brought to you by the Defense Threat Reduction Agency. Part of the U.S. Department of Defense,...
Sep 24, 2017•1 hr 33 min•Ep. 460
The TWiV team reviews the first FDA approved gene therapy, accidental exposure to poliovirus type 2 in a manufacturing plant, and production of a candidate poliovirus vaccine in plants. Hosts: Vincent Racaniello , Dickson Despommier , Alan Dove , Rich Condit , and Kathy Spindler Become a patron of TWiV! Links for this episode ASM Conference on Viral Manipulation of Nuclear Processes ASM Public Outreach Fellowship Kymriah approved (PennMed) CAR T cells (NCBI) Cost of Kymriah (NYTimes) Accidental ...
Sep 17, 2017•1 hr 45 min•Ep. 459
