Synthetic Screens, Genes, and De Novo Proteins: Multiplexed Gene Fragments Drive De Novo Protein Design

Synthetic technologies allow scientists to venture into uncharted waters, asking unique research questions and finding previously unattainable solutions to some of life’s biggest mysteries. From gene editing to protein engineering, synthesized DNA libraries enable researchers to grasp once unreachable high-throughput screening applications and dismantle barriers between experimental ideation and execution. In this podcast series, Synthetic Screens, Genes, and De Novo Proteins, The Scientist’s Creative Services Team talks to experts about their experiences implementing Twist Bioscience’s synthesized long double-stranded gene pools, called Multiplexed Gene Fragments, for high-throughput screening.

In this episode, Niki Spahich from The Scientist spoke with Jeffrey Chang, a graduate student in Nick Polizzi’s laboratory at Harvard Medical School and the Dana Farber Cancer Institute, about designing ligand-binding proteins from scratch and testing them in a high-throughput manner using synthetic DNA libraries.