Medizin - Open Access LMU - Teil 04/22

Precursor proteins are transported into mitochondria in the absence of proteolytic cleavage of the additional sequences

Many nuclear-coded mitochondrial proteins are synthesized as larger precursor polypeptides that are proteolytically processed during import into the mitochondrion. This processing appears to be catalyzed by a soluble, metal-dependent protease localized in the mitochondrial matrix. In this report we employ an in vitro system to investigate the role of processing in protein import. Intact Neurospora crassa mitochondria were incubated with radiolabeled precursors in the presence of the chelator o-p...

Receptor sites involved in posttranslational transport of apocytochrome c into mitochondria

Assembly of cytochrome c involves a series of steps: synthesis of apocytochrome c on free ribosomes, specific binding of apocytochrome c to the mitochondrial surface, transfer across the outer membrane, covalent addition of protoheme, refolding of the polypeptide chain, and association of holocytochrome c with its functional sites at the inner membrane. The binding step of apocytochrome c to Neurospora crassa mitochondria was studied by inhibiting the subsequent transfer steps with the heme anal...

Transport of the precursor to neurospora ATPase subunit 9 into yeast mitochondria

Isolated yeast mitochondria were able to take up Neurospora ATPase subunit 9 in vitro although the homologous yeast protein is synthesized within the mitochondria and inserted into the membrane from the matrix side (Tzagoloff, A., and Meagher, P. (1972) J. Biol. Chem. 247, 594- 603). The transfer of the protein was dependent on an energized mitochondrial inner membrane. It was accompanied by proteolytic processing of the precursor to the mature protein with the correct NH2 terminus as determined...

Transfer of proteins into mitochondria

The precursor form of Neurospora crassa mitochondrial ADP/ATP carrier synthesized in a cell-free protein-synthesizing system can be imported into isolated mitochondria. If the mitochondrial transmembrane potential is abolished, import does not occur but the precursor binds to the mitochondrial surface. Upon reestablishment of the membrane potential, the bound precursor is imported. This occurs without dissociation of the bound precursor from the mitochondrial surface. We conclude that the bindin...

Quantitative correlation of cardiac glycoside binding to its receptor and inhibition of the sodium pump in chicken heart cells in culture

Verminderte in vitro Freisetzung von Elastase aus Granulozyten bei Sepsis nach abdominalchirurgischen Operationen

Inter-alpha-Trypsin Inhibitor of Human Serum

Release of Granulocyte Proteinases during Hemodialysis

Epithelial character and morphological diversity of cell cultures from human amnotic fluids examined by immunofluorescence microscopy and gel electrophoresis of cytoskeletal proteins

UV micro-irradiation of the Chinese hamster cell nucleus and caffeine post-treatment immunocytochemical localization of DNA photolesions in cells with partial and generalized chromosome shattering

UV micro-irradiation of a small part of the Chinese hamster nucleus and caffeine post-incubation often results in shattered chromosomes at the first post-irradiation mitosis. In some of these mitotic cells, chromosome shattering is restricted to a few chromosomes spatially related in a small area of the metaphase spread; in others, shattering includes the whole chromosome complement. These 2 types of damage have been called partial and generalized chromosome shattering (PCS and GCS). Using antis...

Immunocytochemical localization of chromatin regions UV-microirradiated in S phase or anaphase : Evidence for a territorial organization of chromosomes during cell cycle of cultured Chinese hamster cells

Chinese hamster cells (M3-1 line) in S phase were laser-UV-microirradiated (λ, 257 nm) at a small site of the nucleus. Cells were fixed either immediately thereafter or in subsequent stages of the cell cycle, including prophase and metaphase. The microirradiated chromatin was visualized by indirect immunofluorescence microscopy using antibodies specific for UV-irradiated DNA. During the whole post-incubation period (4–15 h) immunofluorescent labelling was restricted to a small part of the nucleu...

Proteinasen und Proteinaseinhibitoren im Ejakulat bei Adnexaffektionen des Mannes

Hämodynamik und Pathobiochemie ausgewählter Plasmaproteine beim hyperdynamen septischen Schock

Plasma levels of neutrophil elastase-Alpha1-proteinase inhibitor complexes and factor XIII (including subunits A and S) in septicemia and leucemia

Proteinases and their inhibitors in inflammation

Plasma levels of human granulocytic elastase-alpha1-proteinase inhibitor complex (E-alpha1PI) in patients with septicemia and acute leukemia

Increased levels of two distinct elastase-like hydrolases in plasma during septicemia

Elastase-alpha1-Proteinaseinhibitor-Komplex (E-alpha1PI)

Keine Wirkungsunterschiede zwischen biosynthetischem Humaninsulin (BHI) und Schweineinsulin (PI) im GCIIS-gesteuerten Insulinhypoglykämietest (IHT)

Computer-assisted method for evaluation and documentation of data from glucose controlled insulin infusion Systems in critically ill patients

Fortschritte in der Therapie endokriner Erkrankungen.

Models of cellular radiation action

Comments on "An Assessment of the Role of Microdosimetry in Radiobiology" (by Dudley T. Goodhead)

Small-Sample Properties of Censored-Data Rank Tests

On the Number of Clumps Resulting from the Overlap of Randomly Placed Figures in a Plane

When two-dimensional figures, called laminae, are randomly placed on a plane domains result that can either be aggregates or individual laminae. The intersection of the union, U, of these domains with a specified field of view, F, in the plane is considered. The separate elements of the intersection are called clumps; they may be laminae, aggregates or partial laminae and aggregates. A formula is derived for the expected number of clumps minus enclosed voids. For bounded laminae homeomorphic to ...

The kinematic fundamental formula - A theorem with implications to dosimetry and microdosimetry

Zur Technik der klinischen Pankreastransplantation: Ist die Okklusionsmethode sinnvoll?

Allgemeine Physiologie der Schilddrüse unter besonderer Berücksichtigung alimentärer Jodmangelzustände

Auto-antibody profiles in patiens with Diabetes melitus, determined by means of an improved fluorescent antibody microtechnic

Zur Kritik der Strumaepidemiologie