Cell fate from Either ageing associated senescence to programmed cell death OR cell proliferation is finely tuned to the splice variant expression of Cell Cycle dependent kinase inhibitors and the relative production of pro-inflammatory cytokines under the synchrony of DNA Damage Repair (DDR), telomerase expression decline, and the protective role of the Senescence Associated Secretory Phenotype (SASP). Dr. Daniel J. Guerra. 06 Dec 2021 Authentic Biochemistry Productions References: Oncotarget. ...
Dec 07, 2021•30 min
Age Ageing , Volume 48, Issue 6, November 2019, Pages 776–782 Biomedicines. 2020 Aug 6;8(8):277
Nov 25, 2021•30 min
Dr. Guerra authentically arrives at the inveighing cancer paradigm contribution to the sickness unto death.
Nov 21, 2021•29 min
Nutrients. 2020 Mar; 12(3): 622. Biochemistry and Cell Biology of Ageing: Part II Clinical Science. 2019. pp 21-43.The Immune System and Its Dysregulation with Aging Ludmila Müller, Svetlana Di Benedetto, and Graham Pawelec. Springer Publishers
Nov 18, 2021•29 min
1.Biochemistry and Cell Biology of Ageing: Part II Clinical Science. 2019. pp 21-43. The Immune System and Its Dysregulation with Aging. Ludmila Müller, Svetlana Di Benedetto, and Graham Pawelec. Springer Publishers. 2. Aging (Albany NY). 2019 Apr 30; 11(8): 2343–2351. 3. Immun Ageing. 2020; 17: 2
Nov 14, 2021•30 min
References Front. Immunol., 29 June 2012 J Immunol 2016;197:3762-3770 Front. Immunol., 03 February 2020
Nov 09, 2021•30 min
The Src-family kinase Lck regulates T-cell development, activation, proliferation, and immune synapse dynamics. These are the initiation phases of TCR signaling. Upon antigen presentation via the APC- TCR immune synapse, Lck phosphorylates tyrosine residues within the immunoreceptor tyrosine-based activation motifs (ITAMs) of the TCR-associated CD3 membrane protein domains. Phosphorylated ITAMs function as a molecular platform for tandem SH2 domains located on the tyrosine kinase Zap-70, initial...
Oct 27, 2021•29 min
Dr Guerra carries out the synthesis of multiple pathobiochemical events into an architechtonic of the aging human co-morbidity resulting in a sickness unto death. Authentic Biochemistry Production 22 October 2021 References Cardiovasc Diabetol. 2020; 19: 33. Cytometry A. 2006 Mar;69(3):189-91 Nat Rev Dis Primers. 2019 Mar 7;5(1):16 J Lipid Res. 2007 Jan;48(1):19-29. doi: 10.1194 Mech Ageing Dev. 2001 Sep 15;122(13):1413-30...
Oct 22, 2021•30 min
That there are senescence-associated decreases in the JAK-STAT signaling transduction cascade has been observed in human lymphocyte lineages. This phenomena, as associated with a decreased JAK- tyrosine phosphorylation of STAT5, is linked to plasma membrane cholesterol content. Therefore, plasma membrane cholesterol content is involved in T cells modulation and proliferation. Acid sphingomyelinase- mediated ceramide lipid raft mobilization and aggregation of membrane receptors plays a crucial ro...
Oct 20, 2021•30 min
Chromatin instability leads to alterations in gene expression that re-directs cellular senescence by corruption of chemokine and cytokine signalling resulting in epithelial to mesenchymal transitions and the potential for metastatic cancer in the elderly . As lymphocyte lineage's age these underlying pathobiochemical events are potentiated by hypo and hyper immune responses thus generating a downward reductio to misdirected hyperinflammatory states and the potential for autoimmune disease sequal...
Oct 16, 2021•30 min
Front Cell Dev Biol . 2019; 7: 226 Advances in Immunology. 2011 v.109.Pp125 Cell Reports 10, 2043–2054, March 31, 2015 Experimental & Molecular Medicine volume 51, pages1–15 (2019)
Oct 14, 2021•30 min
Dr. Guerra details the aging immune phenotype at the transcriptional and metabolic level. References Immun Ageing. 2020; 17: 2. Experimental & Molecular Medicine volume 51, Article number: 80 (2019) Nat Immunol. 2011 Nov 6; 12(12): 10 Cell 146, 772–784, September 2, 2011 Nat Immunol. 2014 Aug; 15(8): 767–776. Cell Reports Volume 17, Issue 11, 13 December 2016, Pages 2827-2836
Oct 05, 2021•30 min
ILC3 can contribute to the progression and aggravation of IBD's while both IL-22 and IL-17, along with IFN-γ, are overexpressed by the dysregulation of NCR− ILC3 or NCR+ ILC3 function NCR (natural cytotoxicity receptors), are type 1 transmembrane proteins of the immunoglobulin superfamily. Upon stimulation, NCR's mediate NK killing and release of IFNγ. They bind viral ligands such as hemagglutinins, neuraminidases, bacterial ligands, and cellular ligands related with auto immune diseases, tumour...
Oct 04, 2021•30 min
Omega-Three VLCPUFA's as asymmetrically distributed in membrane glycerolphospholipids mediate vesiculation and permeability of endothelia to circulating lymphocytes and leukocytes thus coupling folic acid, Vitamin B12 and epigenetic modification of chromatin to ultimately link bioenergetics with cytotoxicity and inflammation. This regulated system involves the neuroimmunoepigenome and the senescent environment of aging human vasculature, skeletal and cardiac muscle, and the CNS toward pathobioch...
Sep 29, 2021•30 min
Pro-inflammatory gene transcription is tanked when demethylation is inhibited but cytotoxicity of virally infected or cancer cells by NK’s (where demethylation is inhibited) remains intact. References J. Biol. Chem. 2018;293:2422-2437 J. of Biol. Chem. 2016. 291, 23756-23768
Sep 28, 2021•30 min
Dr. Guerra discusses some of the complexity of both the epigenetic mechanisms and cellular responses associated with immunomodulatory failure in the aging human. References Anal Cell Pathol (Amst). 2018; 2018: 787.1814. Daniel J. Guerra. 2018. DOI: 10.5772/intechopen.82176 https://www.intechopen.com/chapters/64596
Sep 28, 2021•30 min
Dr. Guerra continues a dialectical accounting of the biochemical and cellular events leading to the terminal outcome of aging. Topic today is the reintroduction of Natural Killer Cells and the epigenetic mechanisms that orchestrate unique gene expression. References Anal Cell Pathol (Amst). 2018; 2018: 787.1814. The Journal of Biological Chemistry2018. 293: 2422-2437
Sep 26, 2021•30 min
Oxidases, dehydrogenases and oxygenases in the electron transport chain et al.
Sep 24, 2021•29 min
This is the link to my new (second in the series) of synoptic Human Aging YouTube lectures. https://youtu.be/IckyMGw8Ffs Please subscribe to both my podcast and YouTube channels! And please contribute! It is the First day of Autumn. Enjoy!
Sep 23, 2021•5 min
Interferons are innate immune agents of inflammation and recovery. Mutations or epigenetic modifications associated with advanced aging may corrupt interferon pathway fidelity involving mitophagy leading to autoimmune disease and morbidity later in life. References Nat Immunol. 2021 Mar; 22(3): 312–321 J Neuroinflammation. 2019; 16: 236.
Sep 22, 2021•30 min
A general audio lecture involving a molecular interactome mediating the innate immune response and the control over T and B lymphocyte memory cells resulting in long term potentiation of autoimmune disease during aging.
Sep 20, 2021•30 min
I discuss the complex but potent interactions of TLR signaling with plasma membrane protein complexes including semaphorin cytoplasmic kinase interactions leading to PPAR gamma transcription that fires off CD36 and beta oxidation gene transcription to switch M1 to M2 macrophage polarity thus feedback regulating the pro-inflammatory glycolytic mToRC system dynamics. Aging is associated with a corruption in this fine-tuning that presents in the older sedentary human individual as a simple dysregul...
Sep 19, 2021•30 min
Macrophage polarization and differentiation works in conjunction with CD4+ T lymphocyte differentiation into Th1 and Th2 subclasses and both NK and B cell activities. The aging human loses feedback control over the pro-inflammatory and anti-inflammatory pathways from these classical cellular differentiations contributing to hyperinflammation and/or hypoinflammatory responses ultimately resulting in pathological cell degeneration or proliferation, morbidity and death. Dr. Daniel J. Guerra Authent...
Sep 18, 2021•30 min
https://youtu.be/4sW-BNVhzrs
Sep 17, 2021•2 min
Dr. Guerra introduces a primer on membrane biochemistry in preparation for a florid analysis of autophagy and the various forms of programmed cell death linked to the aging human. References Cell Discovery volume 7, Article number: 4 (2021)
Sep 13, 2021•30 min
Non coding RNA may regulate melanogenesis and in combination with plasma cell producing IgG the nutritional regulation of satiety. Aging results in alterations of this complex system thus resulting in localized melanogenesis and a corruption of the HPA axis. References: Aging (Albany NY). 2021 Jan 31; 13(2): 2700–2726. Stem Cells Int. 2020; 2020: 1047896. First Published online 2020 Jul 8 PNAS. 2002 Dec 24;99(26):17155-60
Sep 09, 2021•30 min
Components of metabolic sequence toward gluconeogenesis in the human liver.
Sep 07, 2021•30 min
Dr. Guerra details the glucagon and epinephrine mediated control through Stimulatory G-protein couple adenylate cyclase activating PKA over Hepatic Glycolysis and Gluconeogenesis.
Sep 05, 2021•30 min
Utilization of a protein cysteine SH derived thioester intermediate alters the free energy hurdle for the synthesis of 1,3 bis PGA thus allowing for glycolytic ATP and NADH synthesis.
Aug 27, 2021•30 min
The interaction of glycolysis and lactic acid transport from skeletal muscle to the liver plays a significant role in oxygen uptake, transport and release in association with pulmonary carbon dioxide efflux . Dr. Daniel J. Guerra. An Authentic Biochemistry Production 19.8.21.
Aug 20, 2021•30 min
