February 2023 The papers behind the pod: Hobbiesiefken U et al . (2023). Rating enrichment items by female group-housed laboratory mice in multiple binary choice tests using an RFID-based tracking system. PloS one 18(1): e0278709. doi: 10.1371/journal.pone.0278709 Petkov C et al . (2022). Unified ethical principles and an animal research ‘Helsinki’ declaration as foundations for international collaboration. Current Research in Neurobiology 3:100060. doi: 10.1016/j.crneur.2022.100060 Weber E et a...
Feb 16, 2023•4 min•Ep. 2
January 2023 The papers behind the pod: Cassotta M et al . (2022). A worldwide survey on the use of animal-derived materials and reagents in scientific experimentation. Engineering in Life Sciences 22(9):561-604. doi: 10.1002/elsc.202100167 Nuber M et al . (2022). Development of a 3D simulator for training the mouse in utero electroporation. PLOS One 17(12): e0279004. doi: 10.1371/journal.pone.0279004 Dickmann J et al. (2022). Clicker Training Mice for Improved Compliance in the Catwalk Test. An...
Jan 19, 2023•5 min•Ep. 1
December 2022 The papers behind the pod: Menéndez ABC et al . (2022). Creating a kidney organoid-vasculature interaction model using a novel organ-on-chip system. Scientific Reports 12:20699. doi: 10.1038/s41598-022-24945-5 Karp N et al. (2022). A qualitative study of the barriers to using blinding in in vivo experiments and suggestions for improvement. PLOS Biology 20(11): e3001873. doi: 10.1371/journal.pbio.3001873 Talbot S et al . (2022). RELSA—A multidimensional procedure for the comparative...
Dec 15, 2022•4 min•Ep. 12
November 2022 The papers behind the pod: Aulehner K et al . (2022). Grimace scale, burrowing, and nest building for the assessment of post-surgical pain in mice and rats—A systematic review. Frontiers in Veterinary Science 9:930005. doi: 10.3389/fvets.2022.930005 Hair K et al . (2022). ‘Living’ evidence frameworks for in vivo animal research: towards translational evidence-based medicine. BMJ Evidence-Based Medicine 2022;27:A17. doi: 10.1136/ebm-2022-EBMLive.31 Wong J et al . (2022). Electrochem...
Nov 17, 2022•4 min•Ep. 11
October 2022 The papers behind the pod: Piper, SK et al. (2022). Statistical review of animal trials—A guideline. Biometrical Journal https://doi.org/10.1002/bimj.202200061 Gargaté MJ et al. (2022). Parallel Propagation of Toxoplasma gondii In Vivo, In Vitro and in Alternate Model: Towards Less Dependence on the Mice Model. Pathogens https://doi.org/10.3390/pathogens11091038 Mieske P et al. et al. (2022). Bored at home?—A systematic review on the effect of environmental enrichment on the welfare...
Oct 20, 2022•4 min•Ep. 10
September 2022 The papers behind the pod: Pinho RH et al. (2022). Validation of the rabbit pain behaviour scale (RPBS) to assess acute postoperative pain in rabbits ( Oryctolagus cuniculus ). PLoS ONE 17(5): e0268973. https://doi.org/10.1371/journal.pone.0268973 Stull C, Heagerty A and Coleman K (2022). Video Conference Technology as a Tool for Pair Introduction in Rhesus Macaques. Animals 12(14): e1783. https://doi.org/10.3390/ani12141783 Zentrich E et al. (2022). Postoperative Severity Assessm...
Sep 15, 2022•5 min•Ep. 9
August 2022 The papers behind the pod: Arjmand S et al. (2022). Tips and traps for behavioural animal experimentation. Acta Neuropsychiatrica , in press. https://doi.org/10.1017/neu.2022.4 Wrede P et al. (2022). Real-time 3D optoacoustic tracking of cell-sized magnetic microrobots circulating in the mouse brain vasculature. Science Advances 8(19). https://doi.org/10.1126/sciadv.abm9132 Russell A et al. (2022). Reduced housing density improves statistical power of murine gut microbiota studies. C...
Aug 18, 2022•4 min•Ep. 8
July 2022 The papers behind the pod: Ferreira DA et al . (2021). Alternative to Soft Lithography for the Fabrication of Organ-on-a-Chip Elastomeric-Based Devices and Microactuators. Advanced Science 8:2003273. https://doi.org/10.1002/advs.202003273 Eigel D et al . (2019). Cryogel scaffolds for regionally constrained delivery of lysophosphatidylcholine to central nervous system slice cultures: A model of focal demyelination for multiple sclerosis research. Acta Biomaterialia 97:216. https://doi.o...
Jul 21, 2022•4 min•Ep. 7
June 2022 The papers behind the pod: Williams WO and Baneux P (2022). Humane Intervention Points: Refining endpoint terminology to incorporate non-euthanasia intervention options to improve animal welfare and preserve experimental outcomes. Laboratory Animals , in press. https://doi.org/10.1177/00236772221090801 Osorio D et al. (2022). scTenifoldKnk: An efficient virtual knockout tool for gene function predictions via single-cell gene regulatory network perturbation. Patterns 3(3): e100434. http...
Jun 16, 2022•4 min•Ep. 6
May 2022 The papers behind the pod: Wang, Q et al. (2022). Assessment of a 3D neural spheroid model to detect pharmaceutical-induced neurotoxicity. ALTEX 39. https://doi.org/10.14573/altex.2112221 Reynolds P.S. (2022) Between two stools: preclinical research, reproducibility, and statistical design of experiments. BMC Res. Notes 15 , 73. https://doi.org/10.1186/s13104-022-05965-w Tappe-Theodor, A. et al. (2022) The “WWHow” Concept for Prospective Categorization of Post-operative Severity Assessm...
May 19, 2022•4 min•Ep. 5
April 2022 The papers behind the pod: Nigri M et al. (2022). Role of Environment and Experimenter in Reproducibility of Behavioral Studies With Laboratory Mice. Frontiers in Behavioural Neuroscience 16: e835444. https://doi.org/10.3389/fnbeh.2022.835444 Vámos A et al. (2022) Mitophagy Mediates the Beige to White Transition of Human Primary Subcutaneous Adipocytes Ex Vivo . Pharmaceuticals 15(3): 363. https://doi.org/10.3390/ph15030363 Ratuski AS and Weary DM (2022) Environmental Enrichment for R...
Apr 21, 2022•4 min•Ep. 4
The papers behind the pod: 1. Baran SW et al. (2022). Perspectives on the Evaluation and Adoption of Complex In Vitro Models in Drug Development: Workshop with the FDA and the Pharmaceutical Industry (IQ MPS Affiliate). ALTEX, in press. https://doi.org/10.14573/altex.2112203 2. Borba JVB et al. (2022) STopTox: An in Silico Alternative to Animal Testing for Acute Systemic and Topical Toxicity. Environmental Health Perspectives 130(2). https://doi.org/10.1289/EHP9341 3. van der Velden J et al. (20...
Mar 17, 2022•4 min•Ep. 3
February https://doi.org/10.1186/s12915-021-01184-0 https://doi.org/10.1177/00236772211065920 https://doi.org/10.1038/s41598-021-04502-2 It’s the 3rd Thursday of February, and you’re listening to 3 Minute 3Rs. This month, we’re bringing you 3 refinement papers. Let’s start with rodent housing. Research rodents are conventionally housed in shoebox sized cages that limit their ability to perform natural behaviors such as nesting and burrowing. These restrictions are known to impair welfare, but co...
Feb 17, 2022•4 min•Ep. 2
The papers behind the pod: 1. https://doi.org/10.7554/eLife.71601 & https://doi.org/10.7554/eLife.67995 2. https://doi.org/10.3389/fnins.2021.805679 3. https://doi.org/10.1038/s41598-021-98356-3 It’s the 3rd Thursday of January – happy new year! You’re listening to 3 Minute 3Rs, your monthly recap of efforts to replace, reduce and refine the use of animals in research. Of course, we focus on those three Rs, but many have suggested adding a fourth R to the list: reproducibility. Designing exp...
Jan 20, 2022•4 min•Ep. 1
Sedation is necessary for zebrafish during procedures such as imaging, biopsy, and surgery to ensure animal welfare and high-quality science. But the effects of sedation can last beyond the administration period and should also be carefully considered. A paper by Gressler et al. explores the use of eugenol and propofol during a 3-hour sedation and their subsequent effects after a 1 hour washout period. Both drugs had effects on behavior and physiology even after the washout period. For behavior,...
Dec 16, 2021•5 min•Ep. 12
The papers behind the pod: 1. A non-human primate in vitro functional assay for the early evaluation of TB vaccine candidates. npj Vaccines , January 2021. https://doi.org/10.1038/s41541-020-00263-7 2. Inducible dominant negative ErbB2 rat spermatogonial line for generation of transgenic rat model and dissecting ERBB2 tyrosine kinase mediated pathways. Experimental Oncology , June 2019. https://doi.org/10.32471/exp-oncology.2312-8852.vol-41-no-2.13026 3. Characterization of drug responses of min...
Nov 18, 2021•5 min•Ep. 11
The papers behind the pod: 1. Professional Quality of Life in Research Involving Laboratory Animals. Animals 11(9), 2639 (2021) https://doi.org/10.3390/ani11092639 2. Improving culture of care through maximising learning from observations and events: Addressing what is at fault. Laboratory Animals (2021). https://doi.org/10.1177%2F00236772211037177 3. Epidemiology and reporting characteristics of preclinical systematic reviews. PLOS Biology (2021). https://doi.org/10.1371/journal.pbio.3001177 Tr...
Oct 21, 2021•5 min•Ep. 10
Sept 2021 You’re listening to the September episode of 3 Minute 3Rs. The papers behind the pod: Performance of preclinical models in predicting drug-induced liver injury in humans: a systematic review. Scientific Reports 11 , 6403 (2021) https://doi.org/10.1038/s41598-021-85708-2 Ratlas-LH: An MRI template of the Lister hooded rat brain with stereotaxic coordinates for neurosurgical implantations Brain and Neuroscience Advances (2021) https://doi.org/10.1177/23982128211036332 O mouse, where art ...
Sep 16, 2021•4 min•Ep. 9
You’re listening to the August episode of 3 Minute 3Rs! The papers behind the pod: Refinement of the spinal cord injury rat model and validation of its applicability as a model for memory loss and chronic pain. Heliyon 7, E07500 (2021) https://doi.org/10.1016/j.heliyon.2021.e07500 Strategies to encourage the adoption of social housing during cardiovascular telemetry recordings in non-rodents. Journal of Pharmalogical & Toxicological Methods 108 , 106969 (2021). https://doi.org/10.1016/j.vasc...
Aug 19, 2021•4 min•Ep. 8
It’s the 3rd Thursday of July and you’re listening to 3 Minute 3Rs, your monthly recap of efforts to replace, reduce, and refine the use of animals in research. The papers behind the pod: SARS-CoV-2 infects an upper airway model derived from induced pluripotent stem cells. Stem Cells https://stemcellsjournals.onlinelibrary.wiley.com/doi/full/10.1002/stem.3422 A break from the pups: The effects of loft access on the welfare of lactating laboratory rats. PLos One https://doi.org/10.1371/journal.po...
Jul 15, 2021•4 min•Ep. 7
You’re listening to the June 2021 episode of 3 Minute 3Rs The papers behind the pod: Winning paper: Pellegrini L et al. (2020). Human CNS barrier-forming organoids with cerebrospinal fluid production. Science 369(6500): eaaz5626. doi.org/10.1126/science.aaz5626 . Highly commended: Ashworth J et al. (2020). Peptide gels of fully-defined composition and mechanics for probing cell-cell and cell-matrix interactions in vitro. Matrix Biology 85-86: 15-33. doi.org/10.1016/j.matbio.2019.06.009 . [Transc...
Jun 17, 2021•3 min•Ep. 6
You’re listening to the April episode of 3 Minute 3Rs. The papers behind the pod: 1. PCR Testing of Media Placed in Soiled Bedding as a Method for Mouse Colony Health Surveillance. JAALAS https://doi.org/10.30802/AALAS-JAALAS-20-000096 2. New mosquito repellency bioassay for evaluation of repellents and pyrethroids using an attractive blood-feeding device. Parasites & Vectors https://doi.org/10.1186/s13071-021-04656-y 3. Lung cancer organoids analysed on microwell arrays predict drug respons...
May 20, 2021•4 min•Ep. 5
You’re listening to the April episode of 3 Minute 3Rs. The papers behind the pod: 1. Identification of individual zebrafish ( Danio rerio) : a refined protocol for VIE tagging whist considering animal welfare and the principles of the 3RS. Animals 11, 616 (2021) https://doi.org/10.3390/ani11030616 2. Enrichment for laboratory zebrafish – a review of the evidence and challenges. Animals 11, 698 (2021) https://doi.org/10.3390/ani11030698 3. Research relevant background lesions and conditions: ferr...
Apr 15, 2021•3 min•Ep. 4
You’re listening to the March episode of 3 Minute 3Rs. The papers behind the pod: 1. Novel three-dimensional biochip pulmonary sarcoidosis model. PLoS One https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0245805 2. A new group housing approach for non-human primate metabolism studies. Journal of Pharmacological and Toxicological Methods https://doi.org/10.1016/j.vascn.2020.106947 3. The contribution of environmental enrichment to phenotypic variation in mice and rats. eNeuro htt...
Mar 18, 2021•4 min•Ep. 3
You’re listening to the February episode of 3 Minute 3Rs. The papers behind the pod: 1. In Vitro Tests for Assessing the Neutralizing Ability of Snake Antivenoms: Toward the 3Rs Principles. Frontiers in Immunology https://doi.org/10.3389/fimmu.2020.617429 2. The Impact of Acute Loud Noise on the Behavior of Laboratory Birds. Frontiers in Veterinary Science https://doi.org/10.3389/fvets.2020.607632 3. A multicomponent screen for feeding behavior and nutritional status in Drosophila to interrogate...
Feb 18, 2021•4 min•Ep. 2
You’re listening to the January episode of 3 Minute 3Rs. The papers behind the pod: 1. Internal consistency and compatibility of the 3Rs and 3Vs principles for project evaluation of animal research. Laboratory Animals https://doi.org/10.1177/0023677220968583 2. Noise and Vibration in the Vivarium: Recommendations for Developing a Measurement Plan. JAALAS https://doi.org/10.30802/AALAS-JAALAS-19-000131 3. A human tissue screen identifies a regulator of ER secretion as a brain-size determinant. Sc...
Jan 21, 2021•4 min•Ep. 1
You’re listening to the December episode of 3 Minute 3Rs. The papers behind the pod: 1. Protective cranial implant caps for macaques. Journal of Neuroscience Methods https://doi.org/10.1016/j.jneumeth.2020.108992 2. The ‘Cage Climber’ – A new enrichment for use in large-dimensioned mouse facilities. Applied Animal Behaviour Science https://doi.org/10.1016/j.applanim.2020.105078 3. Skin swabbing is a refined technique to collect DNA from model fish species. Scientific Reports https://www.nature.c...
Dec 17, 2020•4 min•Ep. 12
You’re listening to the November episode of 3 Minute 3Rs. The papers behind the pod: 1. Three-Dimensional Human Alveolar Stem Cell Culture Models Reveal Infection Response to SARS-CoV-2. Cell Stem Cell https://doi.org/10.1016/j.stem.2020.10.004 2. Improving reproducibility in animal research by splitting the study population into several ‘mini-experiments’. Sci Rep https://doi.org/10.1038/s41598-020-73503-4 3. Three Pillars of Automated Home-Cage Phenotyping of Mice: Novel Findings, Refinement, ...
Nov 19, 2020•5 min•Ep. 11
You’re listening to the October episode of 3 Minute 3Rs. The papers behind the pod: 1. The epidemiology of fighting in group-housed laboratory mice. Scientific Reports https://www.nature.com/articles/s41598-020-73620-0 2. Development of a miniaturized 96-Transwell air–liquid interface human small airway epithelial model. Scientific Reports https://www.nature.com/articles/s41598-020-69948-2 3. Publication rate in preclinical research: a plea for preregistration. BMJ Open Science https://openscien...
Oct 15, 2020•4 min•Ep. 10
You’re listening to the September episode of 3 Minute 3Rs. The papers behind the pod: 1. Multicellular 3D Neurovascular Unit Model for Assessing Hypoxia and Neuroinflammation Induced Blood-Brain Barrier Dysfunction. Scientific Reports https://www.nature.com/articles/s41598-020-66487-8 2. Automated and rapid self-report of nociception in transgenic mice. Scientific Reports https://www.nature.com/articles/s41598-020-70028-8 3. Zebrafish as an alternative animal model in human and animal vaccinatio...
Sep 17, 2020•4 min•Ep. 9
