Tiermedizin - Open Access LMU

T cell development in the chicken

Characterization of avian natural killer cells and their intracellular CD3 protein complex

Natural killer (NK) cell activity appears to be conserved throughout vertebrate development but NK cells have only been well characterized in mammals. Candidate NK cells have been identified in the chicken as cytoplasmic CD3+ and surface T cell receptor (TCR)/CD3- (TCRO) lymphocytes that often express CD8. The fact that the TCRO cells are abundant in the embryonic spleen before T cells enter this organ allowed us to cultivate the embryonic TCRO cells using growth factors derived from activated a...

Identification of T-cell receptor a-chain genes in the chicken

T-cell receptor (TCR) -chain (TCR) and ß-chain (TCRß) genes are well characterized in mammals, while only TCRß genes have been identified in other vertebrates. To identify avian TCR genes, we used monoclonal anti-CD3 antibodies to isolate chicken TCR for peptide sequence analysis. Degenerate oligonucleotide probes were then used to isolate a candidate TCR cDNA clone that hybridized with a 1.7-kb mRNA species present only in ß T cells and in tissues populated by these cells. Southern blot analysi...

Bovine rotavirus V1005 a P5, not a P12, type like all viruses in a German survey

Bovine rotavirus (BRV) V1005, like 34 further cell culture-adapted strains in a 6-year survey in Upper Bavaria, Germany, is not a P12 but a P5 P-type rotavirus. The conclusion is based on dot blot hybridization with P1-, P5-, and P11-specific cDNAs, encompassing the VP8* region of major sequence diversity, and on PCR using P1-, P5-, and P11-specific primer pairs derived from the VP5* region of VP4 (VP5* and VP8*, respectively, are the larger and smaller tryptic cleavage products of VP4). Sequenc...

Molekularbiologisches Nachweisverfahren zur Differenzierung des in Deutschland als Lebendimpfstoff verwendeten equinen Herpesvirus-Stammes RacH

Der klinische Fall

Mesolithic fishing at the confluence of the Nile and the Atbara (Central Sudan)

Archaic Milet

Eine kleine Knochen- und Molluskenansammlung aus dem Gelände einer villa rustica bei Oberndorf am Lech, Landkreis Donau-Ries

Zur Pathologie, Diagnostik und Therapie der Kokzidiose bei Wiederkäuern im Zoo

Warum Wiedereinbürgerung?

Neue Raubtieranlagen in Hellabrunn

Congenital Hypotrichosis in a female Rottweiler

Histology of the canine claw

Erfahrungen bei der Therapie der Enzootischen Bronchopneumonie von Mastrindern mit einem neuen Makrolidantibiotikum - Eine Feldstudie

Die Beurteilung der Körperkondition (Body condition scoring) im Herdenmanagement

Untersuchungen über den prä- und postoperativen Verlauf von pH-Wert und Netto-Säure-Basen-Ausscheidung im Harn von Kühen mit Labmagenverlagerung

Comparative postcranial osteomorphology and osteometry of springbok, Antidorcas marsupialis (Zimmerman, 1780) and grey rhebok, Pelea capreolus (Forster, 1790) (Mammalia: Bovidae)

Late Quaternary Mammalian remains from Central and Eastern Sudan and their palaeoenvironment Significance

Zum Vorkommen des Arcus scleralis beim Elefanten

Grundsätze zur Diagnose der aviären Mykobakteriosen

Die Verwendung von Tiletamin/Zolazepam/Romifidine zur Distanzimmobilisation von Wildschweinen

Das uveodermatologische Syndrom beim Hund

Eliminationskinetik einiger klinisch relevanter Enzyme im Blutplasma von Rindern nach intravenöser Bolus-Infusion homologer Präparate

Beeinflussung der Konzentration von Kreatinin und Natrium in Blutserum und Harn sowie der fraktionellen Elimination von Natrium durch Elektrolyttherapie bei Kälbern

Intravenöse Infusion flüchtiger Fettsäuren als Stoffwechselbelastungstest zur Überprüfung möglicher Butafosfanwirkungen auf den Energiestoffwechsel des Rindes

Isolation of an Avianlike Group A Rotavirus from a Calf with Diarrhea

An atypical group A rotavirus (993/83) was isolated from a 3-day-old German calf with diarrhea. It differed from 35 conventional German bovine rotavirus isolates analyzed previously with respect to subgroup (strain 993/83 was non-subgroup I and non-subgroup II), serotype (strain 993/83 showed a two-way cross-reaction with serotype 7 and a one-way cross-reaction with serotype 3), and electropherotype (strain 993/83 showed comigrating gene segments 10 and 11). Isolate 993/83 reacted with only one ...

Rotavirus 993/83, isolated from calf faeces, closely resembles an avian rotavirus

Polypeptides from purified virions of the calf rotavirus (RV) isolate 993/83 and those from the pigeon RV isolate PO-13 comigrated on SDS-polyacrylamide gels. Two polypeptides of 45K and 47K were detected at the position of VP6. Both proteins behaved like authentic VP6 protein with EDTA and heat treatment. RV 993/83 and PO-13 showed identical one-dimensional peptide maps for VP2, and the 45K and 47K proteins. More than 70% of sera from German cattle older than 1 year showed neutralizing serum an...

Rapid identification and differentiation of the vaccine strain Rac H from EHV 1 field isolates using a non-radioactive DNA probe

A method for rapid differentiation between the EHV 1 live vaccine strain Rac H and field isolates is described. Total DNA was isolated from virus-infected small scale cell cultures. DNA fragments digested with restriction endonuclease BamHI were separated, transfered and immobilized on filter membranes. A Digoxigenin-labeled probe derived from EHV 1 was used for hybridization. This probe hybridized specifically to sequences of the inverted terminal repeat region which in case of Rac H include a ...

Untersuchungen zur BHV 1-Virusausscheidung bei feldvirus-infizierten, korrekt geimpften Milchkühen nach einmaliger Applikation eines Kortikosteroidpräparats