Ep. 56: scGET-seq. Studying chromatin at higher resolution

In this podcast episode, I talk with Francesca Giannese about an exciting new method for studying chromatin at the single-cell level called scGET-seq. Unlike previous methods such as scATAC-seq, which only allow us to study open chromatin, scGET-seq provides insights into both open and closed chromatin. This breakthrough can lead to a deeper understanding of how chromatin structure impacts gene regulation in individual cells. Tune in to learn more about how scGET-seq works and its potential applications in research.

Contacts for support on scGET-seq implementation:

* Francesca Giannese (wet-lab protocol implementation - enzyme requests)

* Davide Cittaro (data analysis)

More information about scGET-seq

* scGET-seq Wikipedia

* Original Paper

Data Analysis

* Analyzing genomic and epigenetic profiles in single cells by hybrid transposase (scGET-seq)

* Analysis and modeling of scGET-seq data 

Application

* Cancer evolution (EMT in pancreatic cancer)