Medizin - Open Access LMU - Teil 03/22

Uptake of Ca2+ by isolated secretory vesicles from adrenal medulla

Intact secretory vesicles isolated from bovine adrenal medulla contain 94 nmol Na+ per mg of protein, and Ca2+ influx into the vesicles is inhibited by increasing concentrations of extravesicular Na+ (but not of K+, Li+ or choline+) or by addition of the Na+ ionophore monensin. Thus Ca2+ influx is determined by the Na+ gradient across the vesicular membrane. Half maximal inhibition of Ca2+ influx occurs with 34 mM Na+ extravesicularly. The fact that Ca2+ can also be released from the vesicles by...

Liquorproteine bei der Neuromyelitis optica

Es werden zwei Fälle von Neuromyelitis optica beschrieben, die jeweils eine hochgradige Erhöhung der Proteine im Liquor aufwiesen. Während ein Patient nach 6monatigem Krankheitsverlauf starb und pathologisch-anatomisch untersucht werden konnte, überlebte der andere Patient mit erheblichen Residuen. Dieser Fall zeichnete sich dadurch aus, daß deutlich erhöhte IgA- und IgM-Werte sowohl im Liquor als auch im Serum vorlagen. Es wird diskutiert, ob die gefundenen Liquorveränderungen möglicherweise ei...

Lipomatosis symmetrica benigna und neurologische Komplikationen bei chronischem Alkoholismus

Erniedrigung des Biotins als möglicher Faktor im Wirkmechanismus von Antiepileptika

Involvement of Mhc Loci in immune responses that are not Ir-gene-controlled

Twenty-nine randomly chosen, soluble antigens, many of them highly complex, were used to immunize mice of two strains, C3H and B10.RIII. Lymphnode cells from the immunized mice were restimulated in vitro with the priming antigens and the proliferative response of the cells was determined. Both strains were responders to 28 of 29 antigens. Eight antigens were then used to immunize 11 congenic strains carrying different H-2 haplotypes, and the T-cell proliferative responses of these strains were d...

Tauchunfälle - Ursachen und rechtsmedizinische Aspekte

Forensisch-pathologische Aspekte des Hirntraumas

Identifizierung bei Massenkatastrophen

Analyse tödlich verlaufender Ski-Unfälle

T cell-specific suppressor factor(s) with regulatory influence on interleukin 2 production and function

In this study we report that alloantigen-activated spleen cells produce both amplifying and suppressive factors under the same conditions. Both types of soluble mediators--as detected in different assay systems-- were present in the supernatants of in vivo sensitized and in vitro restimulated spleen cell populations and were separable by gel filtration. As shown by others, the amplifying factor (IL 2) was eluted in the size range of 30,000 m.w. The suppressive factor(s) (SF) was eluted in the si...

Frequency analysis of cytolytic T cell precursors (CTL-P) generated in vivo during lethal rabies infection of mice. I. Distinction of CTL-P with different interleukin 2 sensitivity

The aim of this study was to determine the number and state of activity of cytolytic T lymphocytes (CTL) and their precursors (CTL-P) present in vivo during the early stages of viral infection. The local response to lethal infection with rabies virus was used as a model system that is not accessible to analysis by secondary activation in vitro. The local response to alloantigen served as a control. Experimental protocols were established that allow frequency estimates of in vivo antigen-triggere...

Stimulation von TSH durch TRH - ein Vergleich von intravenöser, oraler und intranasaler Applikation

Wertigkeit der direkten Bestimmung des freien Thyroxins im Vergleich zum freien Thyroxin-Index und dem Thyroxin-TBG-Quotienten in der Schilddrüsenfunktionsdiagnostik

Combined HMG/HCG treatment in subfertile men with idiopathic normogonadotropic ogliozoospermia

Urinary cholesterol in cancer screening

Cholesterol determinations in morning urine samples were taken in 235 selected patients with a positive test for microscopic hematuria. Values ranged from 0.2 to 76.0 mg (median 5.5) in 23 patients with urologic malignancies and from 0.1 to 33.4 mg (median 1.1) in 38 patients with various benign diseases of the kidney or urogenital tract. In the 28 patients with urinary tract infections and 146 subjects without evidence of disorders of the kidney and the urogenital system, urinary cholesterol ex...

Results of oral TRH test in the differentiation of compensated and decompensated autonomous thyroid nodules

Plötzlicher, unerwarteter Tod bei seltenen cervicooccipitalen Anomalien und inadäquatem Trauma

Ist die Ozontherapie wirklich ungefährlich?

Bestattungsgesetze und Praxis der Leichenschau

Zur histochemischen Altersbestimmung gedeckter Hirnrindenverletzungen

Zur Verwendbarkeit von Kunststoffen bei der Rekonstruktion der Ohrmuschel

Four porous synthetics were tested for their applicability as cartilage replacement in the reconstruction of the auricle: teflon velour, filamentous dacron, porous polyethylene and proplast II. After formation of a fold, the implants were sutured into subcutaneous cavities of the abdominal skin of rats or fixed with fibrin adhesive. Histological and scanning-electron-microscopic examinations have so far been performed after 1 and 3 months. Form stability with persistence of the desired skin fold...

Probleme der Standardisierung von Digitalispräparaten

Die Entwicklung der Methoden zur physiologischen Wertbestimmung von Digitalispräparaten

A transient outward current in a mammalian central neurone blocked by 4-aminopyridine

It is becoming increasingly clear that nerve cells in the mammalian central nervous system (CNS) have a very complex electroresponsiveness. They exhibit not only time- and voltage-dependent Na+ and K+ conductances, analogous to those in the squid giant axon1, but also a variety of other conductances that have a significant role in the control of cell excitability. Of the outward currents, there are, in addition to the delayed rectifier, the Ca2+-activated K+ current2,3 which underlies the long-l...

Lithium distribution across the membrane of motoneurons in the isolated frog spinal cord

Lithium sensitive microelectrodes were used to investigate the transmembrane distribution of lithium ions (Li+) in motoneurons of the isolated frog spinal cord. After addition of 5 mmol·l–1 LiCl to the bathing solution the extracellular diffusion of Li+ was measured. At a depth of 500 m, about 60 min elapsed before the extracellular Li+ concentration approached that of the bathing solution. Intracellular measurements revealed that Li+ started to enter the cells soon after reaching the motoneuron...

Changes of intracellular sodium and potassium ion concentrations in frog spinal motoneurons induced by repetitive synaptic stimulation

A post-tetanic membrane hyperpolarization following repetitive neuronal activity is a commonly observed phenomenon in the isolated frog spinal cord as well as in neurons of other nervous tissues. We have now used double-barrelled Na+- and K+-ion-sensitive microelectrodes to measure the intracellular Na+- and K+-concentrations and also the extracellular K+-concentration of lumbar spinal motoneurons during and after repetitive stimulation of a dorsal root. The results show that the posttetanic mem...

Convulsant actions of 4-aminopyridine on the guinea-pig olfactory cortex slice

The effects of bath-applied 4-aminopyridine on neurones and extracellular potassium and calcium concentrations were recorded in slices of guinea-pig olfactory cortex. Neurones were orthodromically activated by stimulating the lateral olfactory tract. 4-Aminopyridine (3–10 μM) had the following effects: (1) an increase in the frequency and amplitude of spontaneous postsynaptic potentials: (2) a prolongation and oscillatory behaviour or orthodromically evoked postsynaptic potentials; (3) induction...

Deoxyribonucleic ccid synthesis in isolated chloroplasts and chloroplast extracts of maize

Assembly of Cytochrome c

Transport of apocytochrome c across the outer mitochondrial membrane and conversion to holocytochrome c were studied in vitro. Apocytochrome c was synthesized in a cell-free homogenate from Neurospora crassa. Transfer in vitro was accomplished in a reconstituted system consisting of the postribosomal supernatant of the cell-free homogenate and of isolated and purified mitochondria from Neurospora. The reconstituted system has the following characteristics: * 1. Apocytochrome c is rapidly cleared...

Different Transport Pathways of Individual Precursor Proteins in Mitochondria

Transport of mitochondrial precursor proteins into mitochondria of Neurospora crassa was studied in a cellfree reconstituted system. Precursors were synthesized in a reticulocyte lysate programmed with Neurospora mRNA and transported into isolated mitochondria in the absence of protein synthesis. Uptake of the following precursors was investigated: apocytochrome c, ADP/ATP carrier and subunit 9 of the oligomycin-sensitive ATPase. Addition of high concentrations of unlabelled chemically prepared ...