Let's Talk Micro - podcast cover

Let's Talk Micro

Luis Plazaasm.org
Clinical microbiology explained in simple terms, suitable for students and microbiologists alike. We discuss organisms, reactions, infectious diseases, and more. Hosted by Luis Plaza, a Medical Laboratory Scientist and microbiologist, who also shares his experiences in the field.
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Episodes

Episode 28: Chromogenic agar for MRSA and more

This the last episode of 2021. What is chromogenic agar? In this episode I go over chromogenic agar used to detect MRSA. I also talk about Mannitol salt agar and how it aids in the isolation of Staphylococcus aureus.

Dec 17, 202122 minSeason 1Ep. 28

Episode 26: Staphylococcus aureus

In this episode I talk about Staphylococcus aureus. This is one of the most common organisms seen in Clinical Microbiology and a very serious one. Tune in to find about more about this organism, including its pathogenicity and significance in health care settings. Questions? Feedback? Send those to letstalkmicro@outlook.com Want to support the podcast? Here's how: Venmo: https://venmo.com/u/letstalkmicro Buy me a Ko-fi: https://ko-fi.com/letstalkmicro...

Dec 03, 202124 minSeason 1Ep. 26

Episode 25: MALDI-TOF (part 2)

This episode is part two of a two-part episode. What are the limitations of the Vitek MS? In this episode I go over the limitations of the Vitek MS regarding identification of organisms. I also go over the pros and cons of having this system in the laboratory.

Nov 26, 202130 minSeason 1Ep. 25

Episode 24: MALDI-TOF (part 1)

What is MALDI-TOF? How is it used in Clinical Microbiology?. This is the first part of a two part episode where I discuss MALDI-TOF, and its applications in Clinical Microbiology.

Nov 19, 202123 minSeason 1Ep. 24

Episode 23: Pasteurella, why is it my favorite?

Instagram follower suggestion: After mentioning on a post that Pasteurella was my favorite, a follower suggested for me to make an episode about it. In this episode I talk about Pasteurella, and why do I like it.

Oct 29, 202129 minSeason 1Ep. 23

Episode 22: Anaerobic agar

In this episode I continue going over anaerobic bacteria. Is blood agar sufficient to recover anaerobes? Listen to this episode to learn about different media used for the recovery of anaerobic bacteria.

Oct 22, 202124 minSeason 1Ep. 22

Episode 21: Anaerobes: Sources and incubation

In this episode I continue going over anaerobes. Are all sources acceptable for anaerobic cultures? How do you achieve the anaerobic environment these organisms need to grow? Find out in this episode.

Oct 15, 202127 minSeason 1Ep. 21

Episode 20: An introduction to anaerobes

What is anaerobic bacteria? What is a facultative anaerobe? In this episode I introduce anaerobic bacteria and its relationship to oxygen and growth. Some species are mentioned.

Oct 01, 202126 minSeason 1Ep. 20

Episode 19: Thioglycolate broth

Following up Dania's interview, in this episode I go over thioglycolate broth. What is it used for? Is it helpful in cultures? Listen to find out.

Sep 24, 202127 minSeason 1Ep. 19

Episode 17: Gram negative cocci

In this episode I continue going over tests and biochemicals for gram negative cocci. In addition I briefly go over different species of gram negative cocci and introduce new agar: Martin Lewis and Modified Thayer Martin.

Sep 10, 202125 minSeason 1Ep. 17

Episode 16: Catarrhalis disk

In this episode I start going over tests and biochemicals for gram negative cocci. First up, the catarrhalis disk, used for the identification of Moraxella catarrhalis.

Sep 03, 202125 minSeason 1Ep. 16

Episode 15: Urease test

What is the urease test? What is the principle of it? Is it only used for gram negative rods? In this episode I go over the urea test, including its principle, reactions, and its differences and similarities to the TSI and LIA tests.

Aug 27, 202124 minSeason 1Ep. 15

Episode 14: Lysine Iron Agar

What is the lysine iron agar test? What is it used for? In this episode I go over this test including reactions and comparing and contrasting it with the TSI test.

Aug 20, 202116 minSeason 1Ep. 14

Episode 13: Triple Sugar Iron

What is TSI? Which organisms is it used for? In this episode I go over the Triple Sugar Iron test, including its components, how does it work and examples of organisms and their reactions.

Aug 13, 202126 minSeason 1Ep. 13

Episode 12: Hektoen, XLD and more

In this episode I go over media that Karla Zamora mentioned in her interview such as Hektoen, XLD and Palcam. I discuss components of the agar, colonial morphology and more.

Aug 06, 202125 minSeason 1Ep. 12

Episode 11: A conversation with Karla Zamora

Let's Talk Micro's first interview features a conversation with Karla Zamora, an environmental microbiologist from Mexico. In this episode she talks about her work including techniques and organisms she works with.

Jul 30, 20211 hr 2 minSeason 1Ep. 11

Episode 10: Oxidase

In this episode I continue going over biochemicals for gram negative rods: oxidase. I also briefly discuss some species of gram negative rods and their oxidase results.

Jul 23, 202126 minSeason 1Ep. 10

Episode 9: Indole

In this episode of Let's Talk Micro , we kick off biochemicals for Gram-negative rods with the indole test. After wrapping up the Gram-positive cocci last week, we now explore what indole is, how the test works, and how it helps differentiate organisms like E. coli , Proteus , and Klebsiella . A classic biochemical that still matters in today's microbiology lab. Check out the website: https://www.letstalkmicro.com/ Questions? Feedback? Send those to letstalkmicro@outlook.com Want to support the ...

Jul 16, 202123 minSeason 1Ep. 9

Optochin and Microdase disk

In this episode of Let's Talk Micro , we continue exploring the tools that aid in the identification of gram-positive cocci. Tune in as we discuss the optochin disk, used to help identify Streptococcus pneumoniae , and the Microdase disk test, which supports the identification of Micrococcus species. These simple yet powerful tests remain essential in the clinical microbiology lab. Check out the website: https://www.letstalkmicro.com/ Questions? Feedback? Send those to letstalkmicro@outlook.com ...

Jul 09, 202121 minSeason 1Ep. 8

Episode 7: PYR and Strep Typing

What is the PYR test? What is strep typing? In this episode of Let's Talk Micro , I explain how these tests are used to identify Gram-positive cocci in the lab. Learn what they are, how they work, and why they're important in clinical microbiology. Questions? Feedback? Send those to letstalkmicro@outlook.com Want to support the podcast? Here's how: Venmo: https://venmo.com/u/letstalkmicro Buy me a Ko-fi: https://ko-fi.com/letstalkmicro...

Jul 02, 202125 minSeason 1Ep. 7

Episode 6: Biochemicals- Catalase and Coagulase

What are biochemical tests—and how do they help identify bacteria? In this episode of Let's Talk Micro , I dive into two of the most commonly used biochemicals in clinical microbiology: catalase and coagulase. Learn what they are, how they work, and why they're essential tools in the micro lab. Questions? Feedback? Send those to letstalkmicro@outlook.com Want to support the podcast? Here's how: Venmo: https://venmo.com/u/letstalkmicro Buy me a Ko-fi: https://ko-fi.com/letstalkmicro...

Jun 25, 202124 minSeason 1Ep. 6

Episode 5: 40x Objective- What do we use it for?

What do we use the 40x objective for? How does it compare to the 10x and 100x objectives? In this episode, I break down the differences between these microscope objectives and highlight when and why the 40x objective is used in the clinical microbiology lab. Questions? Feedback? Send those to letstalkmicro@outlook.com Want to support the podcast? Here's how: Venmo: https://venmo.com/u/letstalkmicro Buy me a Ko-fi: https://ko-fi.com/letstalkmicro...

Jun 18, 202127 minSeason 1Ep. 5

Episode 4: Blood Culture Gram Stains

What exactly are blood cultures—and why do they matter? In this episode of Let's Talk Micro , we dive into the critical role of blood culture Gram stains. From how they're reported to how the media is set up, we break down what makes them unique compared to other specimen types. Questions? Feedback? Send those to letstalkmicro@outlook.com Want to support the podcast? Here's how: Venmo: https://venmo.com/u/letstalkmicro Buy me a Ko-fi: https://ko-fi.com/letstalkmicro...

Jun 11, 202127 minSeason 1Ep. 4

Episode 3: Gram Stain morphology and reporting

What do we actually look for when reading a Gram stain? In this episode of Let's Talk Micro , I break down the key morphologies and cellular arrangements you might encounter—and what they mean. From cocci in clusters to rods, we'll cover the essentials and go over standard reporting guidelines to help make sense of what's under the microscope. Questions? Feedback? Send those to letstalkmicro@outlook.com Want to support the podcast? Here's how: Venmo: https://venmo.com/u/letstalkmicro Buy me a Ko...

Jun 04, 202127 minSeason 1Ep. 3

Episode 2- Gram stains

What is a Gram stain and how is it done? In this episode of Let's Talk Micro , I cover the basics of the Gram stain technique—from sample requirements to slide prep and staining. A quick refresher on a core microbiology skill! Questions? Feedback? Send those to letstalkmicro@outlook.com Want to support the podcast? Here's how: Venmo: https://venmo.com/u/letstalkmicro Buy me a Ko-fi: https://ko-fi.com/letstalkmicro...

May 28, 202129 minSeason 1Ep. 2

Episode 1: Media

What exactly is agar, and why is it essential in microbiology? In this episode of Let's Talk Micro , we explore the different types of agar used in the lab—from blood agar to MacConkey—and what they help us detect. Whether you're culturing bacteria or interpreting results, understanding agar is fundamental. Questions? Feedback? Send those to letstalkmicro@outlook.com Want to support the podcast? Here's how: Venmo: https://venmo.com/u/letstalkmicro Buy me a Ko-fi: https://ko-fi.com/letstalkmicro...

May 20, 202122 minSeason 1Ep. 1

An introduction

What is a Medical Laboratory Scientist—and what exactly is microbiology? In this kickoff episode of Let's Talk Micro , I introduce the podcast, share what you can expect from future episodes, and break down some basic microbiology terms. Whether you're a student, professional, or just curious—welcome to the micro-world! Questions? Feedback? Send those to letstalkmicro@outlook.com Want to support the podcast? Here's how: Venmo: https://venmo.com/u/letstalkmicro Buy me a Ko-fi: https://ko-fi.com/l...

May 18, 202110 min
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