Fakultät für Biologie - Digitale Hochschulschriften der LMU - Teil 05/06

Einfluss eines Carboanhydrase XII-spezifischen Antikörpers auf das Tumorwachstum in vitro und in vivo sowie Onkosomen-basierte Generierung neuer tumorreaktiver Antikörper

In dieser Arbeit wurden Mikrovesikel, die von Tumor-Zelllinien in hohen Konzentrationen sekretiert wurden, zur Immunisierung von Ratten verwendet, um auf diese Weise monoklonale Anti-körper gegen membranständige Proteine zu generieren, die auf Tumorzellen vorhanden sind. Der Grund für diesen Ansatz ist, dass Mikrovesikel verschiedenste Membranproteine enthalten, die in Tumorzellen in hohem Maße exprimiert werden. Diese Mikrovesikel sind etwa hundertfach kleiner als die Zellen, denen sie entstamm...

Die Rolle der Checkpoint-Kinase 1 in der Immunglobulin-Diversifizierung

A screen to identify factors in translation-dependent retrograde signaling

Molecular and physiological dissection of GUN1 chloroplast-to-nucleus retrograde signaling

Cross-talk of genetically and environmentally modulated epigenetic factors in the development of anxiety-related behavior

Here, I studied the role of two candidate genes i.e. neuropeptide S receptor 1 (Npsr1) and transmembrane protein 132D (Tmem132d), in two psychopathological animal models of anxiety-related behavior because of recent studies showing importance of these candidates in limbic areas and the frontal cortex of panic disorder patients, respectively. The two animal models are rat (r) and mouse (m), high anxiety-related behavior (rHAB/mHAB) and low anxiety-related behavior(rLAB/mLAB). To understand the an...

Intrinsic and extrinsic regulation of receptor tyrosine kinase signaling for axon growth and guidance

Arbuscular mycorrhizal fungi for reforestation of native tropical trees in the Andes of South Ecuador

WH2 domains and actin variants as multifunctional organizers of the actin cytoskeleton

Actin is one of the most abundant proteins in eukaryotic cells and regulation of the microfilament system is crucial for a wide range of cellular functions including cell shape, cell motility, cell division and membrane dynamics. The aim of this thesis was (1) to gain a better understanding of the function of distinct actin binding domains in the regulation of the actin cytoskeleton and (2) to elucidate the role of actin variants. WH2 domains (WH2, Wiskott-Aldrich syndrome protein homology 2) ar...

Monitoring homology search during DNA double-strand break repair in vivo

Charakterisierung des Imports von Ccs1 und Pet191 in den Intermembranraum durch das Mia40-Erv1-Disulfid-Relay-System

Transient tetraploidy as a route to chromosomal instability

Gc9 is involved in the efficient ring assembly of the chloroplast F0F1 ATP synthase in Arabidopsis

Functional analysis of the role of chloroplasts in senescence

Characterization of anti-apoptotic protein family members (Bax inhibitor-1 and Lifeguard) in Hydra vulgaris

Visualizing T cell activation around the blood-brain barrier Dissertation

T cells recognizing myelin auto-antigens penetrate into the CNS to induce inflammatory autoimmune disease following complex sequential interactions with individual components of the vascular blood-brain barrier (BBB), particularly endothelial cells, and perivascular phagocytes. To determine the functional consequences of these processes, two-photon intravital imaging was performed to compare the behavior of three myelin-specific GFP-expressing T cell lines with different potentials for transferr...

Serielle Analyse stabiler Isotope an Haarkeratin zur post mortem Rekonstruktion von Lebenslaufparametern in forensisch relevanten Fällen von Unterernährung

Die Diagnose einer Unterernährung bei Kindern und Erwachsenen ist in der Pädiatrie und Geriatrie ein wichtiges Thema. Aber auch in der Forensik spielt sie eine große Rolle, gerade im Falle von Vernachlässigung und Missbrauch schutzbefohlener Personen. Derzeit existieren kaum Methoden, um zuverlässig den Zeitrahmen einer Unterernährung rückwirkend zu bestimmen. Die Analyse der stabilen Stickstoff- und Kohlenstoffisotope (δ15N-, δ13C-Wert) im Haarkeratin ist in der Anthropologie ein etabliertes Ve...

Aktivierung des FLT3-Signalweges als molekulare Zielstruktur in der akuten myeloischen Leukämie

Erstellung und Analyse von TALE-Transkriptionsfaktoren mit benutzerdefinierter Erkennungsspezifität

Charakterisierung von Mechanismen der Antigenprozessierung für die Präsentation auf MHC-Klasse-I

Speciation and species delimitation in insular and continental systems

Hintergrund Zu den grundlegendsten Fragestellungen in der Biologie gehört die Frage nach der Natur und Entstehung biologischer Arten. Dieses Problem der Artdefinition (Engl. "Species Problem") war der Ursprung weitläufiger und kontroverser Diskussionen seit der Formulierung der Darwin'schen Evolutionstheorie. Bis heute wurden etwa 30 verschiedene und zum Teil gegensätzliche Konzepte zur Definition und wissenschaftlichen Abgrenzung der Art veröffentlicht. Eine Einigung ist nicht in Sicht. Gleichz...

Conditional in vivo expression of the fusion kinase ITK-SYK

The role of elongation factor EF-P in translation and in copy number control of the transcriptional regulator CadC in Escherichia coli

Enterobacteria have evolved several strategies to survive the acidic environment of the gastrointestinal tract. One of the acid stress resistance systems is the Cad system in Escherichia coli, which is induced by low pH and in the presence of external lysine. It consists of CadA, which catalyzes the decarboxylation of lysine to cadaverine, the lysine/cadaverine antiporter CadB and the pH sensing transcriptional regulator CadC. Moreover, the lysine permease LysP inhibits the induction of cadBA ex...

Functional analysis of two components of the chloroplastic TIC complex

On the trail of anxiety

With the advances in genome wide screening arrays and sequencing techniques scientists were enabled to examine genetic variations and their effects on behavioral phenotypes. While single nucleotide polymorphisms (SNPs) are the most widely studied form of genomic variations to date, another type of variants has become increasingly important in recent research, the copy number variants (CNVs). These large segments of DNA that can comprise up to several megabasepairs and differ in copy number with ...

Function and significance of the putative Na+/solute symporter PutP of Helicobacter pylori

Generation and characterization of heavy chain antibodies derived from Camelids

Antibodies and antibody fragments are essential tools in basic research, diagnostics and therapy. Conventional antibodies consist of two heavy and two light chains with both chains contributing to the antigen-binding site. In addition to these conventional antibodies, camelids (llamas, alpacas, dromedaries and camels) possess so-called heavy chain antibodies (hcAbs) that lack the light chains. The antigen binding site of these unusual antibodies is formed by one single domain only, the so called...

Roquin family proteins control T cell co-stimulation and differentiation

Molecular diversity and phylogeny of the Calcareous Dinophytes (Thoracosphaeraceae, Peridiniales

The LiaFSR three-component System of Bacillus subtilis

Soil bacteria are exposed to constant changes in temperature, moisture, and oxygen content. Additionally, they have to encounter different antimicrobial substances, which are produced by competing bacteria. Those agents often target the bacterial cell envelope, which is an essential structure composed of the cell wall and cell membrane. In order to counteract such life-threatening conditions, bacteria developed signal transducing systems to monitor their environment and to respond signal-specifi...

Monitoring restoration and aboveground biomass in tropical peat swamp forests on Borneo using multi-sensoral remote sensing data